Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,093,363	T→C	R322R (CGT→CGC)	hisC →	histidinol‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,093,363	0	T	C	100.0%	205.6 / NA	57	R322R (CGT→CGC)	hisC	histidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/26);  total (31/26)

AATACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCTTTTTATCGATCGCG  >  NC_000913/2093227‑2093498                                                                                                                                         |                                                                                                                                        aaTACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGAt                                                                                                                                      <  2:952270/140‑1 (MQ=255) aaTACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGAt                                                                                                                                      <  2:887718/140‑1 (MQ=255)  aTACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATc                                                                                                                                     >  2:1027931/1‑140 (MQ=255)        gATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATa                                                                                                                               >  2:77814/1‑140 (MQ=255)        gATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATa                                                                                                                               <  2:700328/140‑1 (MQ=255)           tGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAaac                                                                                                                            <  1:728333/140‑1 (MQ=255)            gCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAaaca                                                                                                                           >  1:714491/1‑140 (MQ=255)             ccGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAaacaa                                                                                                                          <  2:1077506/140‑1 (MQ=255)             ccGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAaacaa                                                                                                                          <  1:458445/140‑1 (MQ=255)                 aCTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCct                                                                                                                      <  2:868282/140‑1 (MQ=255)                     aaaGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCttt                                                                                                                  >  2:1009605/1‑140 (MQ=255)                         agaTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGc                                                                                                              <  2:714491/140‑1 (MQ=255)                           aTCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACNACCCTCTTTAAGccg                                                                                                            <  1:1027931/140‑3 (MQ=255)                             ccccTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGct                                                                                                          >  2:172131/1‑140 (MQ=255)                               ccTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGctgc                                                                                                        >  1:354902/1‑140 (MQ=255)                               ccTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGctgc                                                                                                        >  1:1216249/1‑140 (MQ=255)                                 tGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGctgcct                                                                                                      >  1:839393/1‑140 (MQ=255)                                        agCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAAtt                                                                                               >  2:1022160/1‑140 (MQ=255)                                         gCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTa                                                                                              <  1:172131/140‑1 (MQ=255)                                             gTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGt                                                                                          >  1:1306768/1‑140 (MQ=255)                                                    aCTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAAcc                                                                                   <  2:1256654/140‑1 (MQ=255)                                                     ctctGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAAccc                                                                                  <  1:77814/140‑1 (MQ=255)                                                       ctGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGt                                                                                >  2:1308499/1‑140 (MQ=255)                                                       ctGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGt                                                                                >  2:300721/1‑140 (MQ=255)                                                       ctGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGt                                                                                >  1:1199270/1‑140 (MQ=255)                                                       ctGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGt                                                                                >  1:676624/1‑140 (MQ=255)                                                                aCTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGc                                                                       <  1:1308499/140‑1 (MQ=255)                                                                      ttCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAgcgc                                                                 >  1:512121/1‑140 (MQ=255)                                                                           gcgcgcTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCAt                                                            >  1:379656/1‑140 (MQ=255)                                                                            cgcgcTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCAtt                                                           <  1:572373/140‑1 (MQ=255)                                                                            cgcgcTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCAtt                                                           <  2:676624/140‑1 (MQ=255)                                                                            cgcgcTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCAtt                                                           <  2:1199270/140‑1 (MQ=255)                                                                              cgcTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGa                                                         >  2:695826/1‑140 (MQ=255)                                                                               gcTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGAc                                                        <  1:1223219/140‑1 (MQ=255)                                                                                cTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTg                                                          <  1:694980/137‑1 (MQ=255)                                                                                cTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTg                                                          >  2:694980/1‑137 (MQ=255)                                                                                          tCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTTTCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGc                                             >  2:945323/1‑140 (MQ=255)                                                                                                   gtgtTTAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGt                                    >  1:586600/1‑140 (MQ=255)                                                                                                      tttAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTtg                                 <  2:586600/140‑1 (MQ=255)                                                                                                       ttAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTtga                                <  2:379656/140‑1 (MQ=255)                                                                                                        tAAATCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTtgat                               >  1:238322/1‑140 (MQ=255)                                                                                                         aaaTCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTtgatt                              >  1:393496/1‑139 (MQ=255)                                                                                                          aaTCTTTGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTtgatga                             >  1:923326/1‑140 (MQ=255)                                                                                                            tCTTTGTGGGATCAGGGCATTTTCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGt                           <  1:945323/140‑1 (MQ=255)                                                                                                              tttGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTtga                                >  2:577848/1‑133 (MQ=255)                                                                                                              tttGTGGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTtga                                <  1:577848/133‑1 (MQ=255)                                                                                                                tgtgGGATCAGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGa                       >  2:1179824/1‑140 (MQ=255)                                                                                                                         aGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGt                           <  1:49232/127‑1 (MQ=255)                                                                                                                         aGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGt                           >  2:49232/1‑127 (MQ=255)                                                                                                                         aGGGCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCttt              >  2:45635/1‑140 (MQ=255)                                                                                                                          gggCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGt                           >  2:531479/1‑126 (MQ=255)                                                                                                                          gggCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGt                           <  1:531479/126‑1 (MQ=255)                                                                                                                           ggCATTATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCttttt            <  2:238322/140‑1 (MQ=255)                                                                                                                               ttATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCTTTTTatcg        <  1:422822/140‑1 (MQ=255)                                                                                                                                tATCTTACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCTTTTTatcga       >  1:785760/1‑140 (MQ=255)                                                                                                                                     tACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCTTTTTATCGATcgcg  >  2:697185/1‑140 (MQ=255)                                                                                                                                     tACGCGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCTTTTTATCGATcgcg  >  1:296700/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AATACCTGATTGCCGCACTGAAAGAGATCCCCTGCGTAGAGCAGGTTTTCGACTCTGAAACCAACTACATTCTGGCGCGCTTTAAAGCCTCCAGTGCGGTGTTTAAATCTTTGTGGGATCAGGGCATTATCTTACGTGATCAGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCCTTACGTGCGGAGCAAGTTTGATGAGTCAGAAGTATCTTTTTATCGATCGCG  >  NC_000913/2093227‑2093498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑