Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,163,776	T→C	S301P (TCT→CCT)	baeS →	sensory histidine kinase BaeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,163,776	0	T	C	100.0%	207.6 / NA	59	S301P (TCT→CCT)	baeS	sensory histidine kinase BaeS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/29);  total (30/29)

GGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGT  >  NC_000913/2163646‑2163907                                                                                                                                   |                                                                                                                                    ggTGCTGCGCGGTGAACTGGAAGCCATTCAGGGTGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAg                                                                                                                            <  1:854538/140‑1 (MQ=255) ggTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAg                                                                                                                            <  2:1264884/140‑1 (MQ=255)    gctgcGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGgcg                                                                                                                         >  2:1034254/1‑140 (MQ=255)      tgcGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGct                                                                                                                       >  2:27524/1‑140 (MQ=255)        cgcgGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGctct                                                                                                                     >  2:459767/1‑140 (MQ=255)          cgGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCg                                                                                                                   >  1:457150/1‑140 (MQ=255)            gTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGcc                                                                                                                 <  2:457150/140‑1 (MQ=255)                      aGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAg                                                                                                       <  2:745506/140‑1 (MQ=255)                      aGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAg                                                                                                       <  1:27524/140‑1 (MQ=255)                       gCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGc                                                                                                      >  2:1086968/1‑140 (MQ=255)                         cATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTTGCCTATCAAAAAGCAc                                                                                                    >  2:449565/1‑140 (MQ=255)                         cATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCaaaa                                                                                                         <  2:739628/135‑1 (MQ=255)                         cATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCaaaa                                                                                                         >  1:739628/1‑135 (MQ=255)                         cATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCAc                                                                                                    >  1:204684/1‑140 (MQ=255)                         cATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCAc                                                                                                    >  1:1005008/1‑140 (MQ=255)                            tCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCgg                                                                                                 <  1:1260123/140‑1 (MQ=255)                                   ggCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAg                                                                                                       <  1:807148/127‑1 (MQ=255)                                   ggCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAg                                                                                                       >  2:807148/1‑127 (MQ=255)                                     cgtgcgtAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGa                                                                                        >  2:1088420/1‑140 (MQ=255)                                        gcgtAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTTGCCTATCAAAAAGCACCGGTAGATTTGATcc                                                                                     <  1:449565/140‑1 (MQ=255)                                        gcgtAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATcc                                                                                     <  1:1034254/140‑1 (MQ=255)                                          gtAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCa                                                                                   >  2:272661/1‑140 (MQ=255)                                          gtAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCa                                                                                   <  2:185818/140‑1 (MQ=255)                                          gtAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCa                                                                                   >  2:120038/1‑140 (MQ=255)                                                        gagaCGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCAct                                                                                 >  1:1146687/1‑128 (MQ=255)                                                        gagaCGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCAct                                                                                 <  2:1146687/128‑1 (MQ=255)                                                          gaCGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTggcgg                                                                   <  1:201360/140‑1 (MQ=255)                                                                  cGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGGGgggcc                                                           >  2:35214/1‑139 (MQ=255)                                                                  cGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCa                                                           >  1:168677/1‑140 (MQ=255)                                                                       ttACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGgcggcgggcttcc                                                      >  2:710266/1‑133 (MQ=255)                                                                              cGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGa                                               <  2:349230/140‑1 (MQ=255)                                                                              agacagTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGa                                               <  1:81810/135‑1 (MQ=255)                                                                                gAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCACATTTCGCGAACGAtt                                             >  1:1184400/1‑140 (MQ=255)                                                                                       gTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGGGCATTTTGTGCAAGATTTGCCAGt                                      >  2:601816/1‑140 (MQ=255)                                                                                       gTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCAGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTTGCGAACCATTCGCCAgg                                      >  1:445720/1‑139 (MQ=255)                                                                                           acTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTg                                  >  1:250679/1‑140 (MQ=255)                                                                                           acTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTg                                  >  1:18507/1‑140 (MQ=255)                                                                                              gACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGcc                               <  1:272661/140‑1 (MQ=255)                                                                                              gACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGcc                               <  1:1088420/140‑1 (MQ=255)                                                                                               aCCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTTGCGGACGATTCGCCCGTCGTGGccc                              >  2:43615/1‑139 (MQ=255)                                                                                               aCCAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCt                              >  1:601078/1‑140 (MQ=255)                                                                                                ccAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGGGGCGGGCGGCGCATTTCGCGAAACATTCGCCCGTCGGGGcccg                             >  2:1262194/1‑138 (MQ=255)                                                                                                 cAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGa                            <  2:250679/140‑1 (MQ=255)                                                                                                 cAAACTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGa                            <  2:18507/140‑1 (MQ=255)                                                                                                  aaaCTGGTTGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGGGGGGCCTTTTGCGAACGATTTCCCCGTCGTgggccgga                           >  2:736147/1‑134 (MQ=255)                                                                                                         ttGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTTGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCaa                    >  1:53938/1‑140 (MQ=255)                                                                                                         ttGACGATCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCaa                    >  1:307562/1‑140 (MQ=255)                                                                                                               aTCTCCATCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTcc              <  1:736147/140‑1 (MQ=255)                                                                                                                     aTCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCa        <  2:1092308/140‑1 (MQ=255)                                                                                                                     aTCAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCa        <  1:43615/140‑1 (MQ=255)                                                                                                                       cAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGa      <  2:204684/140‑1 (MQ=255)                                                                                                                       cAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGa      <  1:35214/140‑1 (MQ=255)                                                                                                                       cAGTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGa      <  2:448000/140‑1 (MQ=255)                                                                                                                       aggTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGa      <  1:237490/138‑1 (MQ=255)                                                                                                                         gTTGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACa    <  1:1262194/140‑1 (MQ=255)                                                                                                                          ttGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCCTTTCGCGAACGACTCGCCCGGCGGGGGCCGAAACTGCAATTTTTCCTGCCAGACaa   >  2:1242516/1‑139 (MQ=255)                                                                                                                          ttGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTTGCGAAAGATTCGCCAGTCGTGGCCCGAAACTGCCATTTTTCCTGCCCGACaa   >  2:276944/1‑139 (MQ=255)                                                                                                                          ttGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAg   <  2:601078/140‑1 (MQ=255)                                                                                                                           tGTCGATGCCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGt  <  2:168677/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    GGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCGAACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGT  >  NC_000913/2163646‑2163907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑