Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	181,141	T→C	G86G (GGT→GGC)	degP →	periplasmic serine endoprotease DegP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	181,141	0	T	C	100.0%	241.6 / NA	68	G86G (GGT→GGC)	degP	periplasmic serine endoprotease DegP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/35);  total (33/35)

ATGCTCGAAAAGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGTTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACCAACAACCAC  >  NC_000913/181013‑181276                                                                                                                                 |                                                                                                                                        aTGCTCGAAAAGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCa                                                                                                                              >  2:1055478/1‑140 (MQ=255) aTGCTCGAAAAGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCa                                                                                                                              >  1:785758/1‑140 (MQ=255) aTGCTCGAAAAGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCa                                                                                                                              >  1:709928/1‑140 (MQ=255) aTGCTCGAAAAGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCa                                                                                                                              >  1:196245/1‑140 (MQ=255)   gCTCGAAAAGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGACTCTCCGTTCCaga                                                                                                                            <  1:756399/140‑1 (MQ=255)         aaaGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGCAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCtctc                                                                                                                      >  1:693665/1‑140 (MQ=255)           aGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCg                                                                                                                    <  1:1332246/140‑1 (MQ=255)           aGGTGATGCCATCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTACg                                                                                                                    <  2:415350/140‑1 (MQ=255)                aTGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTg                                                                                                               >  1:221052/1‑140 (MQ=255)                    cTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAg                                                                                                           <  2:709928/140‑1 (MQ=255)                       cAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGt                                                                                                        >  1:431804/1‑140 (MQ=255)                          tGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGc                                                                                                     <  2:693665/140‑1 (MQ=255)                          tGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGc                                                                                                     <  1:470636/140‑1 (MQ=255)                             tCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAg                                                                                                  <  2:99908/140‑1 (MQ=255)                             tCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAg                                                                                                  <  2:46054/140‑1 (MQ=255)                               aGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAggg                                                                                                >  1:687310/1‑140 (MQ=255)                               aGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAggg                                                                                                >  1:265001/1‑140 (MQ=255)                                gCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGc                                                                                               <  1:974110/140‑1 (MQ=255)                                  aTTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCgg                                                                                             >  1:912079/1‑140 (MQ=255)                                           gAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGc                                                                                                     >  1:721492/1‑123 (MQ=255)                                           gAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGc                                                                                                     <  2:721492/123‑1 (MQ=255)                                           gAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCa                                                                                                   >  2:1354622/1‑125 (MQ=255)                                           gAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCa                                                                                                   <  1:1354622/125‑1 (MQ=255)                                           gAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAAtggtgg                                                                                    >  1:969985/1‑140 (MQ=255)                                                tAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGcc                                                                               <  1:876485/140‑1 (MQ=255)                                                 aGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCa                                                                              >  1:479444/1‑140 (MQ=255)                                                 aGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCa                                                                              >  1:50255/1‑140 (MQ=255)                                                 aGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCa                                                                              >  1:1320759/1‑140 (MQ=255)                                                   cacaACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGc                                                                            >  1:1241356/1‑140 (MQ=255)                                                   cacaACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGc                                                                            <  1:257419/140‑1 (MQ=255)                                                    acaACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGACTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCa                                                                           >  1:1216950/1‑140 (MQ=255)                                                       aCCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACa                                                                        <  2:431804/140‑1 (MQ=255)                                                        ccGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGcc                                                                               >  1:515841/1‑132 (MQ=255)                                                        ccGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGcc                                                                               <  2:515841/132‑1 (MQ=255)                                                        ccGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAg                                                                       >  1:1118100/1‑140 (MQ=255)                                                                  gccgcGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATgg                                                             >  1:681436/1‑140 (MQ=255)                                                                    cgcgTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAg                                                                             >  2:462871/1‑122 (MQ=255)                                                                    cgcgTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAg                                                                             <  1:462871/122‑1 (MQ=255)                                                                    cgcgTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCa                                                                <  1:759352/135‑1 (MQ=255)                                                                    cgcgTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCa                                                                >  2:759352/1‑135 (MQ=255)                                                                    cgcgTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGgcg                                                           >  2:436714/1‑140 (MQ=255)                                                                     gcgTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGgcgc                                                          >  1:962199/1‑140 (MQ=255)                                                                      cgTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCt                                                         <  2:974287/140‑1 (MQ=255)                                                                          tGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGt                                                     <  2:912079/140‑1 (MQ=255)                                                                              gcgTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGtt                                                    >  2:726448/1‑137 (MQ=255)                                                                              gcgTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGtt                                                    <  1:726448/137‑1 (MQ=255)                                                                               cgTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCgg                                                <  2:969985/140‑1 (MQ=255)                                                                               cgTAATTTCCAGCAGTTCTTCGGTGATAATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCgg                                                >  2:1002629/1‑140 (MQ=255)                                                                                    tttCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGtca                                           <  2:687310/140‑1 (MQ=255)                                                                                    tttCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGtca                                           <  2:265001/140‑1 (MQ=255)                                                                                             gTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATg                                  <  2:785758/140‑1 (MQ=255)                                                                                              ttcttcGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGc                                               <  1:890902/126‑1 (MQ=255)                                                                                              ttcttcGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGc                                               >  2:890902/1‑126 (MQ=255)                                                                                              ttcttcGGTGATGATTCTCCGTTATGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGc                                 >  2:682710/1‑140 (MQ=255)                                                                                                  tcGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGAt                             <  2:1288087/140‑1 (MQ=255)                                                                                                  tcGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGAt                             <  1:430745/140‑1 (MQ=255)                                                                                                  tcGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGAt                             <  1:1055478/140‑1 (MQ=255)                                                                                                  tcGGTGATAATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGAt                             <  1:1002629/140‑1 (MQ=255)                                                                                                   cGGTGATGATTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCTGCGTCATCATTGATGCCGATa                            <  2:965258/140‑1 (MQ=255)                                                                                                         tgatTCTCCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCt                      <  1:443562/140‑1 (MQ=255)                                                                                                              ctcCGTTCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATgtc                 <  2:681436/140‑1 (MQ=255)                                                                                                                   ttCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCAc            >  1:506028/1‑140 (MQ=255)                                                                                                                    tCTGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCAcc           >  1:124656/1‑140 (MQ=255)                                                                                                                      tGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACcaa         <  2:962199/140‑1 (MQ=255)                                                                                                                      tGCCAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACcaa         >  1:1346681/1‑140 (MQ=255)                                                                                                                        ccAGGAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACcaaca       >  2:69829/1‑140 (MQ=255)                                                                                                                           ggAAGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACCAACAAcc    <  2:1118100/140‑1 (MQ=255)                                                                                                                             aaGGCTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACCAACAACCAc  <  1:436714/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        ATGCTCGAAAAGGTGATGCCTTCAGTGGTCAGCATTAACGTAGAAGGTAGCACAACCGTTAATACGCCGCGTATGCCGCGTAATTTCCAGCAGTTCTTCGGTGATGATTCTCCGTTCTGCCAGGAAGGTTCTCCGTTCCAGAGCTCTCCGTTCTGCCAGGGTGGCCAGGGCGGTAATGGTGGCGGCCAGCAACAGAAATTCATGGCGCTGGGTTCCGGCGTCATCATTGATGCCGATAAAGGCTATGTCGTCACCAACAACCAC  >  NC_000913/181013‑181276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑