Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,440,577	A→G	A343A (GCT→GCC)	fabB ←	beta‑ketoacyl‑[acyl carrier protein] synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,440,577	0	A	G	100.0%	208.1 / NA	58	A343A (GCT→GCC)	fabB	beta‑ketoacyl‑[acyl carrier protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/29);  total (29/29)

GACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACC  >  NC_000913/2440446‑2440711                                                                                                                                    |                                                                                                                                       gACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCTCGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTg                                                                                                                                <  2:1100303/140‑1 (MQ=255)      aaCGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGc                                                                                                                           >  2:61612/1‑140 (MQ=255)             gtCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCt                                                                                                                                     >  1:1047225/1‑123 (MQ=255)             gtCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCt                                                                                                                                     >  2:422898/1‑123 (MQ=255)             gtCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCt                                                                                                                                     <  2:1047225/123‑1 (MQ=255)             gtCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCt                                                                                                                                     <  1:422898/123‑1 (MQ=255)               cAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAgcgc                                                                                                                  <  1:258688/140‑1 (MQ=255)               cAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAgcgc                                                                                                                  <  1:766879/140‑1 (MQ=255)                aGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGcc                                                                                                                 >  2:66771/1‑140 (MQ=255)                 gTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGccc                                                                                                                >  1:607610/1‑140 (MQ=255)                            gTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCcagcag                                                                                                                     >  2:942392/1‑124 (MQ=255)                            gTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCcagcag                                                                                                                     <  1:942392/124‑1 (MQ=255)                                tttCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTc                                                                                                 <  2:607610/140‑1 (MQ=255)                                tttCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTc                                                                                                 <  2:870268/140‑1 (MQ=255)                                   cGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATg                                                                                              >  1:689273/1‑140 (MQ=255)                                   cGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATg                                                                                              >  1:720782/1‑140 (MQ=255)                                       cACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCtt                                                                                          >  1:1203276/1‑140 (MQ=255)                                             gTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTg                                                                                    <  1:61612/140‑1 (MQ=255)                                                    cccGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAAt                                                                             >  1:887055/1‑140 (MQ=255)                                                        cAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGcc                                                                         >  2:736559/1‑140 (MQ=255)                                                        cAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCC‑‑AGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCgg                                                                       >  2:1312655/1‑140 (MQ=255)                                                        cAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAACATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGc                                                                          <  1:423521/139‑1 (MQ=255)                                                        cAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAACATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGc                                                                          >  2:423521/1‑139 (MQ=255)                                                           ccTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCggg                                                                      <  2:385371/140‑1 (MQ=255)                                                               cTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGCCCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGctct                                                                  <  2:1203276/140‑1 (MQ=255)                                                                tCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCgg                                                                       <  2:584350/134‑1 (MQ=255)                                                                tCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCgg                                                                       >  1:584350/1‑134 (MQ=255)                                                                     cAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGc                                                            >  2:300313/1‑140 (MQ=255)                                                                      aGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGcc                                                           <  2:689273/140‑1 (MQ=255)                                                                        ctctTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGa                                                         >  2:661667/1‑140 (MQ=255)                                                                        ctctTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGa                                                         <  2:887055/140‑1 (MQ=255)                                                                         tctTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGTCGGGCTCTTATCGCCGaa                                                        <  1:66771/140‑1 (MQ=255)                                                                         tctTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGaa                                                        <  1:311465/140‑1 (MQ=255)                                                                         tctTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGaa                                                        <  2:1270854/140‑1 (MQ=255)                                                                         tctTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGaa                                                        >  1:1270854/1‑140 (MQ=255)                                                                          ctTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAac                                                       <  2:1244559/140‑1 (MQ=255)                                                                             cAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGaa                                                        <  2:569438/136‑1 (MQ=255)                                                                             cAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGaa                                                        >  1:569438/1‑136 (MQ=255)                                                                                 gTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCAc                                                >  1:708658/1‑140 (MQ=255)                                                                                     aTGCTCGGGGCGATAAAGCCGTGTTCCGGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGAt                                            >  1:58853/1‑140 (MQ=255)                                                                                     aTGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGAt                                            >  1:1025414/1‑140 (MQ=255)                                                                                     aTGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGAt                                            <  1:736559/140‑1 (MQ=255)                                                                                       gCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAg                                          >  2:551324/1‑140 (MQ=255)                                                                                       gCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCC‑‑AGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCt                                        <  1:1312655/140‑1 (MQ=255)                                                                                           ggggCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGc                                      >  1:1348496/1‑140 (MQ=255)                                                                                            gggCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGcc                                     <  2:720782/140‑1 (MQ=255)                                                                                             ggCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCa                                    >  2:1274418/1‑140 (MQ=255)                                                                                                     aGCCGTGTTCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTc                            <  1:1274418/140‑1 (MQ=255)                                                                                                            ttCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGc                     >  2:851520/1‑140 (MQ=255)                                                                                                            ttCCAGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGc                     >  1:729791/1‑140 (MQ=255)                                                                                                                aGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAAc                 <  1:899849/140‑1 (MQ=255)                                                                                                                aGCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACCTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAAc                 <  2:386768/140‑1 (MQ=255)                                                                                                                 gCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAAcc                >  2:768039/1‑140 (MQ=255)                                                                                                                 gCATCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAAcc                >  2:625174/1‑140 (MQ=255)                                                                                                                   aTCAGCAGAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCgg              <  1:768039/140‑1 (MQ=255)                                                                                                                     cagcagAGAGTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCACCAACCGGAg            <  2:1348496/140‑1 (MQ=255)                                                                                                                           agagTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAGCACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAg      <  1:300313/140‑1 (MQ=255)                                                                                                                             agTAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTa    >  2:740106/1‑140 (MQ=255)                                                                                                                               tAGATGGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTAcc  >  1:1042025/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                       GACATAACGGTGGTCAGTTCGCGATCGGTCGTTTCGGTCACGATGTTCAGACCCGCAGCCTGCTCGTCCAGCTCTTCAATGTTGATGCTCGGGGCGATAAAGCCGTGTTCCAGCATCAGCAGAGAGTAGATAGCTTCCTGTACGCCAGCAGCGCCCAGAGAGTGACCGGTCATGGCTTTGGTTGCAGAAATCGCCGGGCTCTTATCGCCGAACACTTCACGGATAGCTGCCAGCTCTTTCACGTCGCCAACCGGAGTCGAAGTACC  >  NC_000913/2440446‑2440711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑