Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,769,953	A→G	H84R (CAC→CGC)	yfjR →	CP4‑57 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,769,953	0	A	G	100.0%	189.6 / NA	53	H84R (CAC→CGC)	yfjR	CP4‑57 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/24);  total (29/24)

GTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTG  >  NC_000913/2769819‑2770080                                                                                                                                       |                                                                                                                                gtCAGTGCGCCCGTTGCGGGGTGATTTTCGTGAACGACTGATGTATCTGGCCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGAGGTGCTTATCTTTGCCCGCCGCa                                                                                                                            <  2:608403/140‑1 (MQ=37) gtCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCcgccgcc                                                                                                                            >  2:118309/1‑139 (MQ=255) gtCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCcgccgc                                                                                                                             >  1:485369/1‑139 (MQ=255) gtCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCcgccgc                                                                                                                             <  2:485369/139‑1 (MQ=255)  tCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAg                                                                                                                           >  2:244307/1‑140 (MQ=255)           aCGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGc                                                                                                                  <  1:115829/140‑1 (MQ=255)           aCGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGc                                                                                                                  >  1:247680/1‑140 (MQ=255)              cTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCgg                                                                                                               >  1:614023/1‑140 (MQ=255)                                       tGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGt                                                                                      <  2:247680/140‑1 (MQ=255)                                         aTGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCt                                                                                    <  2:414467/140‑1 (MQ=255)                                                tGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGAccgcc                                                                                        >  2:656781/1‑129 (MQ=255)                                                tGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGAccgcc                                                                                        <  1:656781/129‑1 (MQ=255)                                                tGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAAt                                                                             >  2:1125833/1‑140 (MQ=255)                                                 ggACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATg                                                                            >  1:468550/1‑140 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGAccgcc                                                                                        >  2:1266707/1‑126 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGAccgcc                                                                                        <  1:1266707/126‑1 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGAccgcc                                                                                        <  1:1118372/126‑1 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGAccgcc                                                                                        >  2:1118372/1‑126 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCa                                                                          >  1:3332/1‑140 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCa                                                                          >  2:1158178/1‑140 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCa                                                                          >  1:989913/1‑140 (MQ=255)                                                   aCCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTT‑CCGGCCTTGACCGCCGTCTGGTGAATGCAc                                                                         >  1:649276/1‑140 (MQ=255)                                                      tGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCActg                                                                       >  1:96583/1‑140 (MQ=255)                                                       ggAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCActgc                                                                      >  2:156710/1‑140 (MQ=255)                                                        gAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCActgct                                                                     >  1:1342468/1‑140 (MQ=255)                                                            aTCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGAtg                                                                 >  1:181949/1‑140 (MQ=255)                                                            aTCAGTCCGAATCCTCCCGCTTACGCACTGCGGGCAGTGAGATGCAGAGGGTGCCCGCCGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGAtg                                                                 <  2:683978/140‑1 (MQ=255)                                                             tCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGAtgt                                                                >  1:1094665/1‑140 (MQ=255)                                                             tCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGAtgt                                                                >  2:472429/1‑140 (MQ=255)                                                             tCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGAtgt                                                                >  2:527955/1‑140 (MQ=255)                                                                gcccGGATACGGCCGCTTACGCACGGCTGGGAGGGAGATGCAGAGGGTGCCCGCCGTGCTTATCTTGGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCg                                                             <  2:96583/138‑1 (MQ=255)                                                                gTCCGGATCCTGCCGCTTACGCCCTGCGGGCAGTGAGATGCAGATGGTGCCCGACGTGTTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCg                                                             <  2:667338/140‑1 (MQ=255)                                                                  ccGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGAt                                                           <  1:156710/140‑1 (MQ=255)                                                                  ccGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGAt                                                           <  2:3332/140‑1 (MQ=255)                                                                     gATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAg                                                        <  1:472429/140‑1 (MQ=255)                                                                      aTACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGt                                                       <  2:1266481/140‑1 (MQ=255)                                                                      aTACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGt                                                       >  1:683978/1‑140 (MQ=255)                                                                         cTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTT‑CCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTcc                                                   <  2:649276/140‑1 (MQ=255)                                                                           gCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCg                                                             <  2:791506/129‑1 (MQ=255)                                                                           gCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCg                                                             >  1:791506/1‑129 (MQ=255)                                                                                  aCGCACTGCGGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGa                                           <  2:275931/140‑1 (MQ=255)                                                                                       ctgctgGCAGGGAGATGCAGATGGTGCCCGACGGGTTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCa                                      <  2:181949/140‑1 (MQ=255)                                                                                               aGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCaa                              >  2:430137/1‑140 (MQ=255)                                                                                                gTGAGATGCAGATGGTGTCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAAt                             >  1:1069935/1‑140 (MQ=255)                                                                                                    gaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCgg                         >  1:137084/1‑140 (MQ=255)                                                                                                    gaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCgg                         >  2:760535/1‑140 (MQ=255)                                                                                                      tGCAGATGGTCCCCGCCGTGTTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGtt                       <  2:1280165/140‑1 (MQ=255)                                                                                                              gTGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGcc               >  2:494187/1‑140 (MQ=255)                                                                                                               tGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCt              >  2:982454/1‑140 (MQ=255)                                                                                                               tGCCCGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCt              >  1:1280165/1‑140 (MQ=255)                                                                                                                   cGACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAg          <  1:494187/140‑1 (MQ=255)                                                                                                                    gACGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAgg         <  1:1083683/140‑1 (MQ=255)                                                                                                                     aCGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCg                 <  1:934401/131‑1 (MQ=255)                                                                                                                     aCGTGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCg                 >  2:934401/1‑131 (MQ=255)                                                                                                                        tGCTTATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCa     <  2:1094665/140‑1 (MQ=255)                                                                                                                           ttATCTTTGCCCGCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCATGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTg  <  1:180806/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                GTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGACTGATGTATCTGGACCTGGAGTATCAGTCCGGATACTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTG  >  NC_000913/2769819‑2770080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑