Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	257,594	G→A	T356T (ACG→ACA)	frsA →	fermentation‑respiration switch protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	257,594	0	G	A	100.0%	284.2 / NA	82	T356T (ACG→ACA)	frsA	fermentation‑respiration switch protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/38);  total (44/38)

AACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTC  >  NC_000913/257458‑257730                                                                                                                                         |                                                                                                                                         aaCAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCa                                                                                                                                       <  2:1233430/140‑1 (MQ=255) aaCAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCa                                                                                                                                       <  1:151089/140‑1 (MQ=255)   cAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCGCAACACCAAt                                                                                                                                     >  1:933180/1‑140 (MQ=255)      gTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAAt                                                                                                                                     >  2:229353/1‑137 (MQ=255)      gTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAAt                                                                                                                                     <  1:229353/137‑1 (MQ=255)      gTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGtt                                                                                                                                  >  1:408622/1‑140 (MQ=255)        gCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTAt                                                                                                                                <  2:494895/140‑1 (MQ=255)         ccGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATc                                                                                                                               >  2:648117/1‑140 (MQ=255)          cGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCa                                                                                                                              >  1:152018/1‑140 (MQ=255)           ggAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAg                                                                                                                             <  2:1358230/140‑1 (MQ=255)             aaaTGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGc                                                                                                                           <  2:333479/140‑1 (MQ=255)                tGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTAc                                                                                                                        >  2:607913/1‑140 (MQ=255)                tGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTAc                                                                                                                        <  2:33871/140‑1 (MQ=255)                    tCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGc                                                                                                                           <  1:559764/133‑1 (MQ=255)                    tCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGc                                                                                                                           >  2:559764/1‑133 (MQ=255)                    tCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTAc                                                                                                                        <  1:882130/136‑1 (MQ=255)                    tCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTAc                                                                                                                        >  2:882130/1‑136 (MQ=255)                       tGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGgaaga                                                                                                                 <  1:412503/140‑1 (MQ=255)                       tGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGgaaga                                                                                                                 >  1:439672/1‑140 (MQ=255)                         aCGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTAt                                                                                                                                <  2:1268249/123‑1 (MQ=255)                         aCGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTAt                                                                                                                                >  1:1268249/1‑123 (MQ=255)                            ttCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGAt                                                                                                            >  2:496236/1‑140 (MQ=255)                             tCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATc                                                                                                           >  2:898606/1‑140 (MQ=255)                              cTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATcc                                                                                                          >  2:961794/1‑140 (MQ=255)                              cTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATcc                                                                                                          >  2:811978/1‑140 (MQ=255)                               tGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCg                                                                                                         >  2:835196/1‑140 (MQ=255)                                      tCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGcc                                                                                                  >  1:1328731/1‑140 (MQ=255)                                      tCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGcc                                                                                                  >  2:132168/1‑140 (MQ=255)                                      tCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGcc                                                                                                  <  1:648117/140‑1 (MQ=255)                                      tCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGcc                                                                                                  >  1:1300592/1‑140 (MQ=255)                                                aTGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGa                                                                                        >  2:965653/1‑140 (MQ=255)                                                  gCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACt                                                                                      <  1:898606/140‑1 (MQ=255)                                                  gCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACt                                                                                      <  2:439672/140‑1 (MQ=255)                                                  gCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACt                                                                                      <  2:64377/140‑1 (MQ=255)                                                   cATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTc                                                                                     >  1:1203561/1‑140 (MQ=255)                                                    atgatgCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCa                                                                                    >  2:820205/1‑140 (MQ=255)                                                     tgatgCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCAc                                                                                   <  1:655272/140‑1 (MQ=255)                                                      gatgCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACg                                                                                  <  1:961794/140‑1 (MQ=255)                                                            tCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAAt                                                                            <  1:220151/140‑1 (MQ=255)                                                             ccGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATc                                                                           >  1:621462/1‑140 (MQ=255)                                                             ccGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATc                                                                           >  1:896606/1‑140 (MQ=255)                                                                aTGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCAcc                                                                        <  1:760416/140‑1 (MQ=255)                                                                  gAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCtc                                                                      >  2:242924/1‑140 (MQ=255)                                                                  gAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCtc                                                                      >  1:400436/1‑140 (MQ=255)                                                                    aGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCtcat                                                                    <  1:965653/140‑1 (MQ=255)                                                                       gTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTcatcat                                                                 <  2:1300592/140‑1 (MQ=255)                                                                       gTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTcatcat                                                                 >  1:467199/1‑140 (MQ=255)                                                                          gcgcGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATctg                                                              >  2:341935/1‑140 (MQ=255)                                                                           cgcgTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATctgc                                                             >  1:463168/1‑140 (MQ=255)                                                                              gTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGa                                                          <  2:467199/140‑1 (MQ=255)                                                                                ggAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACg                                                        <  1:433316/140‑1 (MQ=255)                                                                                 gAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACgg                                                       <  2:1203561/140‑1 (MQ=255)                                                                                   gCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTa                                                     <  1:560553/140‑1 (MQ=255)                                                                                   gCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTa                                                     <  1:607913/140‑1 (MQ=255)                                                                                     tGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTaaa                                                   <  2:470972/140‑1 (MQ=255)                                                                                        aTCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAatta                                                <  2:152018/140‑1 (MQ=255)                                                                                            cTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTag                                            <  1:3201/140‑1 (MQ=255)                                                                                            cTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTag                                            >  1:547843/1‑140 (MQ=255)                                                                                              aTTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTa                                                     >  1:906071/1‑129 (MQ=255)                                                                                              aTTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTa                                                     <  2:906071/129‑1 (MQ=255)                                                                                                    tAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCa                                    >  1:498708/1‑140 (MQ=255)                                                                                                         gTGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTaa                               >  1:823319/1‑140 (MQ=255)                                                                                                          tGCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAAc                              >  2:959811/1‑140 (MQ=255)                                                                                                           gCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGAt                                        <  1:1150005/129‑1 (MQ=255)                                                                                                           gCAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGAt                                        >  2:1150005/1‑129 (MQ=255)                                                                                                            cAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAAttatta                                             <  2:924148/123‑1 (MQ=255)                                                                                                            cAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAAttatta                                             >  1:924148/1‑123 (MQ=255)                                                                                                            cAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATc                                       >  2:1364793/1‑129 (MQ=255)                                                                                                            cAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATc                                       <  1:1364793/129‑1 (MQ=255)                                                                                                            cAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAAccc                            >  1:1183447/1‑140 (MQ=255)                                                                                                            cAAGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAAccc                            >  2:1202604/1‑140 (MQ=255)                                                                                                             aaGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCg                           <  2:896606/140‑1 (MQ=255)                                                                                                              aGGATTGCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCgg                          >  2:1292753/1‑140 (MQ=255)                                                                                                                    gCTGGGACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATc                    >  1:186821/1‑140 (MQ=255)                                                                                                                     cTGGGACGTCGCTGCCCAACACCAATGTTATCAGACTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAACTTATTAGAGATCCCATTTAACCCGGTGTATCg                   >  2:1206880/1‑140 (MQ=255)                                                                                                                         gACGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAAt               <  2:400436/140‑1 (MQ=255)                                                                                                                          aCGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATc                    <  2:922690/134‑1 (MQ=255)                                                                                                                          aCGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATc                    >  1:922690/1‑134 (MQ=255)                                                                                                                           cGTCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAAttt             >  1:1151470/1‑140 (MQ=255)                                                                                                                             tCGCTGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTg           >  1:1097395/1‑140 (MQ=255)                                                                                                                                 tGCCCAACACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACaa       <  2:589205/140‑1 (MQ=255)                                                                                                                                      aaCACCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTc  <  1:341935/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTC  >  NC_000913/257458‑257730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑