Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,855,313	A→G	T326A (ACC→GCC)	fhlA →	DNA‑binding transcriptional activator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,855,313	0	A	G	100.0%	280.8 / NA	78	T326A (ACC→GCC)	fhlA	DNA‑binding transcriptional activator FhlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/44);  total (34/44)

ACGATTTAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAG  >  NC_000913/2855176‑2855447                                                                                                                                          |                                                                                                                                       aCGATTTAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGcc                                                                                                                                      >  2:713821/1‑140 (MQ=255)     tttAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTa                                                                                                                                  >  2:220582/1‑140 (MQ=255)      ttAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTAc                                                                                                                                 <  1:545186/140‑1 (MQ=255)       tAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTAcc                                                                                                                                <  1:277973/140‑1 (MQ=255)       tAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTAcc                                                                                                                                <  1:156192/140‑1 (MQ=255)         gCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCaa                                                                                                                              <  1:653615/140‑1 (MQ=255)         gCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCaa                                                                                                                              <  1:159616/140‑1 (MQ=255)              cTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGa                                                                                                                         <  1:431082/140‑1 (MQ=255)               tATGAACGCATGTTGTTCGATACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTa                                                                                                                                  <  2:156285/130‑1 (MQ=255)               tATGAACGCATGTTGTTCGATACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTa                                                                                                                                  >  1:156285/1‑130 (MQ=255)                 tGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAAtt                                                                                                                      <  2:156633/140‑1 (MQ=255)                   aaCGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTa                                                                                                                    <  2:497079/140‑1 (MQ=255)                    aCGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTAc                                                                                                                   >  2:937269/1‑140 (MQ=255)                       cATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTa                                                                                                                                  <  2:1338710/122‑1 (MQ=255)                       cATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTa                                                                                                                                  >  1:1338710/1‑122 (MQ=255)                       cATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTgc                                                                                                                >  1:974753/1‑140 (MQ=255)                       cATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTgc                                                                                                                >  2:273658/1‑140 (MQ=255)                         tgttgtTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTgcgc                                                                                                              <  2:1170389/140‑1 (MQ=255)                           ttgttCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCa                                                                                                            >  1:1158527/1‑140 (MQ=255)                              ttCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTgcg                                                                                                               <  2:426133/134‑1 (MQ=255)                              ttCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTgca                                                                                                               >  1:426133/1‑133 (MQ=255)                                cGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTgcg                                                                                                               >  2:245019/1‑132 (MQ=255)                                cGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTgcg                                                                                                               <  1:245019/132‑1 (MQ=255)                                 gACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGc                                                                                                      <  2:1134662/140‑1 (MQ=255)                                  acacCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGcc                                                                                                     <  2:355521/140‑1 (MQ=255)                                  acacCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGcc                                                                                                     <  2:504982/140‑1 (MQ=255)                                  acacCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGcc                                                                                                     >  2:466951/1‑140 (MQ=255)                                  acacCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGcc                                                                                                     <  2:473046/140‑1 (MQ=255)                                   cacCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCg                                                                                                    <  2:350945/140‑1 (MQ=255)                                        ggggCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACgt                                                                                               <  1:317590/140‑1 (MQ=255)                                                   aTTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCg                                                                                                    <  2:864127/124‑1 (MQ=255)                                                   aTTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCg                                                                                                    >  1:864127/1‑124 (MQ=255)                                                         aCCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGa                                                                              >  2:845963/1‑140 (MQ=255)                                                           cTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATa                                                                            <  1:1123732/140‑1 (MQ=255)                                                           cTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATa                                                                            <  1:117194/140‑1 (MQ=255)                                                           cTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATa                                                                            <  1:198197/140‑1 (MQ=255)                                                            ttGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATaa                                                                           <  1:273658/140‑1 (MQ=255)                                                            ttGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATaa                                                                           <  1:1270748/140‑1 (MQ=255)                                                             tgtgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAAc                                                                          >  2:885267/1‑140 (MQ=255)                                                              gtgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCaa                                                                                        >  2:112376/1‑125 (MQ=255)                                                              gtgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCaa                                                                                        <  1:112376/125‑1 (MQ=255)                                                              gtgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACg                                                                         >  2:1109872/1‑140 (MQ=255)                                                               tgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGc                                                                        <  1:885267/140‑1 (MQ=255)                                                               tgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGc                                                                        >  2:705777/1‑140 (MQ=255)                                                               tgCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGc                                                                        >  1:136901/1‑140 (MQ=255)                                                                  cTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCt                                                                     <  1:263874/140‑1 (MQ=255)                                                                   tGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCt                                                                     <  2:443298/139‑1 (MQ=255)                                                                   tGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCt                                                                     >  1:443298/1‑139 (MQ=255)                                                                   tGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTc                                                                    <  1:220582/140‑1 (MQ=255)                                                                    gTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCg                                                                   >  2:1274830/1‑140 (MQ=255)                                                                     ttACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGc                                                                  >  2:319938/1‑140 (MQ=255)                                                                        ccGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTa                                                               <  2:583311/140‑1 (MQ=255)                                                                         cGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTAt                                                              >  1:877968/1‑140 (MQ=255)                                                                          gCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATc                                                             <  1:1109872/140‑1 (MQ=255)                                                                          gCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATc                                                             <  2:136901/140‑1 (MQ=255)                                                                              aTGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGa                                                         >  1:411044/1‑140 (MQ=255)                                                                               tGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGaa                                                        >  1:444385/1‑140 (MQ=255)                                                                               tGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGaa                                                        >  1:375363/1‑140 (MQ=255)                                                                                gTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGaaa                                                       >  2:883373/1‑140 (MQ=255)                                                                                    ggCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCa                                                   <  1:937269/140‑1 (MQ=255)                                                                                               gCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAg                                        >  2:1277109/1‑140 (MQ=255)                                                                                               gCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAg                                        >  2:1038702/1‑140 (MQ=255)                                                                                                     cGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGc                                  >  1:589307/1‑140 (MQ=255)                                                                                                        gCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTgg                               <  1:713821/140‑1 (MQ=255)                                                                                                         cTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGaaa                                         <  2:642912/129‑1 (MQ=255)                                                                                                         cTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGaaa                                         >  1:642912/1‑129 (MQ=255)                                                                                                               cTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAGTCCATCGTCTGAAAGAACGGCTGGTtgatga                        <  1:705777/140‑1 (MQ=255)                                                                                                               cTGGCGCAATGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTtgatga                        >  1:886619/1‑140 (MQ=255)                                                                                                                        tGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGc               <  1:319938/140‑1 (MQ=255)                                                                                                                        tGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGc               <  1:170533/140‑1 (MQ=255)                                                                                                                        tGCGAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGc               >  2:1295422/1‑140 (MQ=255)                                                                                                                           gAAGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCt            <  2:252115/140‑1 (MQ=255)                                                                                                                            aaGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGc               >  1:681838/1‑136 (MQ=255)                                                                                                                            aaGAGAAAGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGc               <  2:681838/136‑1 (MQ=255)                                                                                                                                 aaaGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGa      >  1:101113/1‑140 (MQ=255)                                                                                                                                  aaGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGag     <  1:845963/140‑1 (MQ=255)                                                                                                                                  aaGTGTTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGag     <  2:411044/140‑1 (MQ=255)                                                                                                                                     tgtTTGCCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGagcag  <  2:101113/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       ACGATTTAGCCCCCTATGAACGCATGTTGTTCGACACCTGGGGCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGCGAAGAGAAAGTGTTTACCACTACCAATCTGAATTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGCCCTCGCCTATCAGGAAATCCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAG  >  NC_000913/2855176‑2855447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑