Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,039,943	T→C	V30A (GTG→GCG)	fldB →	flavodoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,039,943	0	T	C	98.0%	337.3 / ‑3.5	99	V30A (GTG→GCG)	fldB	flavodoxin 2
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (49/48);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TCTGATACACTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGA  >  NC_000913/3039806‑3040072                                                                                                                                          |                                                                                                                                  tCTGATACACTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGa                                                                                                                                 <  2:1330026/140‑1 (MQ=255)        cacTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACa                                                                                                                          <  2:952990/140‑1 (MQ=255)         acTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACAt                                                                                                                         <  2:638060/140‑1 (MQ=255)         acTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACAt                                                                                                                         <  2:927929/140‑1 (MQ=255)          cTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATa                                                                                                                        >  2:343751/1‑140 (MQ=255)           tAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATaa                                                                                                                       >  1:595633/1‑140 (MQ=255)           tAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATaa                                                                                                                       >  1:994995/1‑140 (MQ=255)             gCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAAcc                                                                                                                     >  2:200091/1‑140 (MQ=255)             gCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAAcc                                                                                                                     >  2:835850/1‑140 (MQ=255)             gCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAAcc                                                                                                                     >  1:70112/1‑140 (MQ=255)             gCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAAcc                                                                                                                     >  1:1168724/1‑140 (MQ=255)             gCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAAcc                                                                                                                     >  1:815834/1‑140 (MQ=255)              ccGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCt                                                                                                                    >  1:219684/1‑140 (MQ=255)               cGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACa                                                                                                                          <  2:338298/133‑1 (MQ=255)               cGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACa                                                                                                                          >  1:338298/1‑133 (MQ=255)               cGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTc                                                                                                                   >  1:819799/1‑140 (MQ=255)               cGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTc                                                                                                                   <  2:1168724/140‑1 (MQ=255)                gCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCa                                                                                                                  >  1:1160426/1‑140 (MQ=255)                 cAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCaa                                                                                                                 >  2:344971/1‑140 (MQ=255)                  aaaaGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACAt                                                                                                                         <  2:1293321/131‑1 (MQ=255)                  aaaaGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACAt                                                                                                                         >  1:424447/1‑131 (MQ=255)                  aaaaGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACAt                                                                                                                         >  1:1293321/1‑131 (MQ=255)                  aaaaGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACAt                                                                                                                         <  2:424447/131‑1 (MQ=255)                           agcagAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTcc                                                                                                       <  2:770748/140‑1 (MQ=255)                                    gagaAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTa                                                                                              <  2:70112/140‑1 (MQ=255)                                     agaAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTaa                                                                                             <  2:702514/140‑1 (MQ=255)                                     agaAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTaa                                                                                             <  2:219684/140‑1 (MQ=255)                                     agaAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTaa                                                                                             <  2:815834/140‑1 (MQ=255)                                     agaAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTaa                                                                                             >  1:1043564/1‑140 (MQ=255)                                       aaGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATg                                                                                           <  1:384688/140‑1 (MQ=255)                                       aaGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATg                                                                                           <  1:678055/140‑1 (MQ=255)                                        aGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATgg                                                                                          <  2:698584/140‑1 (MQ=255)                                            tACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCa                                                                                      >  1:84603/1‑140 (MQ=255)                                            tACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCa                                                                                      >  2:770956/1‑140 (MQ=255)                                               gTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTa                                                                                   <  2:294044/140‑1 (MQ=255)                                                   tGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAt                                                                               <  2:198083/140‑1 (MQ=255)                                                   tGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAt                                                                               >  2:463397/1‑140 (MQ=255)                                                    gAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAtg                                                                              >  1:60554/1‑140 (MQ=255)                                                     aaTATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAtgt                                                                             >  1:640198/1‑140 (MQ=255)                                                       tatGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGc                                                                           <  2:994995/140‑1 (MQ=255)                                                         tGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAg                                                                                        >  1:84218/1‑125 (MQ=255)                                                         tGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAg                                                                                        <  2:84218/125‑1 (MQ=255)                                                         tGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTc                                                                         >  2:212385/1‑140 (MQ=255)                                                         tGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTc                                                                         >  2:1339430/1‑140 (MQ=255)                                                         tGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTc                                                                         >  2:1162993/1‑140 (MQ=255)                                                          gggTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAg                                                                                        >  1:753480/1‑124 (MQ=255)                                                          gggTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAg                                                                                        <  2:753480/124‑1 (MQ=255)                                                          gggTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCa                                                                        >  1:1279222/1‑140 (MQ=255)                                                           ggTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCAt                                                                       >  1:1277575/1‑140 (MQ=255)                                                             tCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAtgtg                                                                            >  1:1026097/1‑133 (MQ=255)                                                             tCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAtgtg                                                                            <  2:1026097/133‑1 (MQ=255)                                                             tCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTc                                                                     >  2:582846/1‑140 (MQ=255)                                                             tCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTc                                                                     >  2:102531/1‑140 (MQ=255)                                                             tCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTc                                                                     >  1:576896/1‑140 (MQ=255)                                                             tCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGAGGTGCTCATTc                                                                     >  1:1269433/1‑140 (MQ=255)                                                              cTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAGAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCAt                                                                       >  1:434467/1‑137 (MQ=255)                                                              cTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAGAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCAt                                                                       <  2:434467/137‑1 (MQ=255)                                                               ttttttACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCt                                                                          <  2:52807/133‑1 (MQ=255)                                                               ttttttACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCt                                                                          >  1:52807/1‑133 (MQ=255)                                                               ttttttACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTg                                                                   >  1:832880/1‑140 (MQ=255)                                                                tttttACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTgg                                                                  <  2:1279222/140‑1 (MQ=255)                                                                    tACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTAt                                                              >  2:512810/1‑140 (MQ=255)                                                                     aCGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATc                                                             <  1:212385/140‑1 (MQ=255)                                                                           ccAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGCGCTCATTCTGGGTATCCCGAcc                                                       >  1:960277/1‑140 (MQ=255)                                                                              gCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTgg                                                    <  1:344971/140‑1 (MQ=255)                                                                                   tGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGAttt                                               <  2:232682/140‑1 (MQ=255)                                                                                   tGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGAttt                                               <  1:770956/140‑1 (MQ=255)                                                                                    gTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGAtttt                                              >  1:852109/1‑140 (MQ=255)                                                                                       acacCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGt                                           <  1:835850/140‑1 (MQ=255)                                                                                       acacCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGt                                           <  2:1043564/140‑1 (MQ=255)                                                                                             aaaTGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATc                                     >  1:912357/1‑140 (MQ=255)                                                                                             aaaTGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATc                                     >  2:599428/1‑140 (MQ=255)                                                                                             aaaTGGCGGCAGAAAAAATCCGCGATATTATCGGCACAGAACTGGCGACCTTACATAACCTCAAGGACAACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATc                                     >  1:166399/1‑140 (MQ=255)                                                                                               aTGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCa                                   >  1:433069/1‑140 (MQ=255)                                                                                                tGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAg                                  <  1:463397/140‑1 (MQ=255)                                                                                                tGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAg                                  <  1:343751/140‑1 (MQ=255)                                                                                                   cggcAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTTGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGaa                               <  2:680031/140‑1 (MQ=255)                                                                                                    ggcAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAg                              >  1:1077826/1‑140 (MQ=255)                                                                                                     gcAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGCGACCCTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGa                             >  1:190498/1‑140 (MQ=255)                                                                                                          aaaaaTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTggg                        <  2:98629/140‑1 (MQ=255)                                                                                                            aaaTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATACCCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGaa                      <  2:1269433/140‑1 (MQ=255)                                                                                                            aaaTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGaa                      <  2:576896/140‑1 (MQ=255)                                                                                                             aaTCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAg                     <  2:84603/140‑1 (MQ=255)                                                                                                             aaTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAg                     <  1:200091/140‑1 (MQ=255)                                                                                                              aTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGc                    <  2:819799/140‑1 (MQ=255)                                                                                                              aTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGc                    <  1:1339430/140‑1 (MQ=255)                                                                                                              aTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGc                    <  1:102531/140‑1 (MQ=255)                                                                                                              aTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGc                    <  2:640198/140‑1 (MQ=255)                                                                                                              aTCCGCGATATTATCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGc                    <  1:599428/140‑1 (MQ=255)                                                                                                                         tatCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATc         <  2:832880/140‑1 (MQ=255)                                                                                                                         tatCGGCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATc         >  1:762091/1‑140 (MQ=255)                                                                                                                              gCCCAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTc    >  2:1092256/1‑140 (MQ=255)                                                                                                                               cccAGAACTGGCGACCCTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcg   <  2:190498/140‑1 (MQ=255)                                                                                                                                ccAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTggg            <  1:268725/130‑1 (MQ=255)                                                                                                                                ccAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTggg            >  2:268725/1‑130 (MQ=255)                                                                                                                                ccAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTggg            <  1:1129382/130‑1 (MQ=255)                                                                                                                                ccAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTggg            >  2:1129382/1‑130 (MQ=255)                                                                                                                                ccAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcga  >  2:718920/1‑140 (MQ=255)                                                                                                                                ccAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcga  <  2:433069/140‑1 (MQ=255)                                                                                                                                ccAGAACTGGCGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcga  >  2:1211463/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  TCTGATACACTAGCCGCAAAAGCCACAGCAGAATCGAGAAGCTTACGTTATGAATATGGGTCTTTTTTACGGTTCCAGCACCTGTTACACCGAAATGGCGGCAGAAAAAATCCGCGATATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGA  >  NC_000913/3039806‑3040072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑