BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,092,075	G→A	W66* (TGG→TGA)	gshB →	glutathione synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,092,075	0	G	A	99.1%	355.0 / NA	107	W66* (TGG→TGA)	gshB	glutathione synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (66/40); minor base T (0/1); total (66/41)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

AGTTTTGCTATGTTGCTGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCT  >  NC_000913/3091938‑3092209

aGTTTTGCTATGTTGCTGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGAtt                                                                                                                                      >  2:1018456/1‑140 (MQ=255)

 gTTTTGCTATGTTGCTGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGAttt                                                                                                                                     >  2:1302358/1‑140 (MQ=255)

       cTATGTTGCTGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTttcgttc                                                                                                                               >  1:1304615/1‑140 (MQ=255)

         aTGTTGCTGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTtcgttcgt                                                                                                                             <  2:550138/140‑1 (MQ=255)

               cTGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGa                                                                                                                       <  1:476637/140‑1 (MQ=255)

                tGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGaa                                                                                                                      <  1:1302358/140‑1 (MQ=255)

                  gAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACa                                                                                                                    <  1:288819/140‑1 (MQ=255)

                      cacaGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGAt                                                                                                                >  2:1103578/1‑140 (MQ=255)

                      cacaGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGAt                                                                                                                >  2:1280100/1‑140 (MQ=255)

                         aGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTg                                                                                                             >  1:1296074/1‑140 (MQ=255)

                            gtcgtGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAAc                                                                                                                     <  1:726040/129‑1 (MQ=255)

                            gtcgtGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAAc                                                                                                                     >  2:726040/1‑129 (MQ=255)

                            gtcgtGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCg                                                                                                          <  1:1185520/140‑1 (MQ=255)

                            gtcgtGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCg                                                                                                          >  1:1095581/1‑140 (MQ=255)

                                tGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTgg                                                                                                      >  1:660211/1‑140 (MQ=255)

                                 ggTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGc                                                                                                     <  1:1103578/140‑1 (MQ=255)

                                 ggTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGc                                                                                                     <  2:180501/140‑1 (MQ=255)

                                 ggTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGc                                                                                                     <  1:1280100/140‑1 (MQ=255)

                                  gTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGcc                                                                                                    >  1:164983/1‑140 (MQ=255)

                                   ttACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCg                                                                                                   <  1:402382/140‑1 (MQ=255)

                                   ttACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCg                                                                                                   <  2:1304615/140‑1 (MQ=255)

                                   ttACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCg                                                                                                   >  2:402382/1‑140 (MQ=255)

                                        aaCTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGAtctc                                                                                              >  2:169378/1‑140 (MQ=255)

                                            tCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGAtg                                                                                          <  1:169378/140‑1 (MQ=255)

                                            tCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGAtg                                                                                          <  1:1018456/140‑1 (MQ=255)

                                            tCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGACCTGCCGCTGGCCGATCTCGAtg                                                                                          <  2:1284581/140‑1 (MQ=255)

                                             cACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGATCTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGAtgt                                                                                         >  1:28404/1‑140 (MQ=255)

                                             cACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGAtc                                                                                                >  2:640683/1‑133 (MQ=255)

                                             cACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGAtc                                                                                                <  1:640683/133‑1 (MQ=255)

                                             cACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGAtgt                                                                                         >  1:467440/1‑140 (MQ=255)

                                             cACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGAtgt                                                                                         >  2:1113307/1‑140 (MQ=255)

                                                   atGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCt                                                                                   >  1:612626/1‑140 (MQ=255)

                                                     ggAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGa                                                                                 <  2:660211/140‑1 (MQ=255)

                                                      gagaTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGAt                                                                                <  1:1113307/140‑1 (MQ=255)

                                                       agaTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCatgatg                                                                               >  1:798351/1‑140 (MQ=255)

                                                       agaTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATg                                                                               >  2:714116/1‑140 (MQ=255)

                                                         aTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGAtct                                                                                               >  1:911795/1‑122 (MQ=255)

                                                         aTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGAtct                                                                                               <  2:911795/122‑1 (MQ=255)

                                                          tGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGt                                                                            >  1:1287319/1‑140 (MQ=255)

                                                            ggCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTaa                                                                          >  1:763803/1‑140 (MQ=255)

                                                            ggCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTaa                                                                          >  1:1346285/1‑140 (MQ=255)

                                                            ggCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTaa                                                                          >  1:1111831/1‑140 (MQ=255)

                                                            ggCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTaa                                                                          >  1:1065101/1‑140 (MQ=255)

                                                                   tGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATc                                                                                     <  1:888923/122‑1 (MQ=255)

                                                                   tGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATc                                                                                     >  2:888923/1‑122 (MQ=255)

                                                                   tGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACccg                                                                   <  2:164983/140‑1 (MQ=255)

                                                                           aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACccg                                                                   >  2:905543/1‑132 (MQ=255)

                                                                           aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACccg                                                                   <  1:905543/132‑1 (MQ=255)

                                                                           aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGa                                                           >  1:419568/1‑140 (MQ=255)

                                                                           aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGa                                                           >  1:429164/1‑140 (MQ=255)

                                                                           aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGa                                                           >  2:597654/1‑140 (MQ=255)

                                                                           aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGa                                                           >  2:1034408/1‑140 (MQ=255)

                                                                               aTGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATAcc                                                       >  2:501578/1‑140 (MQ=255)

                                                                               aTGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATAcc                                                       >  1:57817/1‑140 (MQ=255)

                                                                                 ggTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGa                                                     >  1:738071/1‑140 (MQ=255)

                                                                                  gTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAg                                                    >  2:650096/1‑140 (MQ=255)

                                                                                     aaGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGttt                                                 >  1:550268/1‑140 (MQ=255)

                                                                                     aaGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGttt                                                 >  1:442470/1‑140 (MQ=255)

                                                                                     aaGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGttt                                                 >  2:812466/1‑140 (MQ=255)

                                                                                         ccGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCt                                             >  1:1224513/1‑140 (MQ=255)

                                                                                          cgcgCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTa                                            >  2:435865/1‑140 (MQ=255)

                                                                                            cgcCCATCCCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcg                                          <  1:326002/140‑1 (MQ=255)

                                                                                            cgcCCATACCCGCACGCTGAACGTGAAGCAGATCTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcg                                          <  2:28404/140‑1 (MQ=255)

                                                                                            cgcCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcg                                          >  1:145644/1‑140 (MQ=255)

                                                                                            cgcCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcg                                          <  1:395457/140‑1 (MQ=255)

                                                                                            cgcCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcg                                          >  2:367771/1‑140 (MQ=255)

                                                                                             ccccATACCCGCACGCTAAGCGTGAAGCAGACCTACGAAGAGTGTTTTTCTTGTGTCGGTAAACAGGTTCTGCCGCGGGCCGATCTCGCTGTAACCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgc                                         <  2:429164/139‑1 (MQ=255)

                                                                                             cccaatccccgcccgCTGAACGTGAAGCAAAACAACGAAGATTGATTTTCGTTCTTCGTTGAACAGAATCCGCCGCTGCCCGATCTCGATGGGATCCTATTCGGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgc                                         <  2:500326/133‑1 (MQ=255)

                                                                                                cATCCCCGCACGCGGAACGTGAAGCAGAACGACGAAGAGTGTTTTTCGTTCGTGGGTAAACAGGATCTGCCGCTGGCTGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGAc                                      <  2:1296074/140‑1 (MQ=255)

                                                                                                  tccccgcccgCTGAAGGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                    <  2:145644/138‑1 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgc                                         >  2:71536/1‑135 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgc                                         <  1:71536/135‑1 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcg                                          >  2:1306977/1‑134 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcg                                          <  1:1306977/134‑1 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                    >  2:901947/1‑140 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                    <  2:738071/140‑1 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                    <  2:1111831/140‑1 (MQ=255)

                                                                                                  tACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                    <  1:367771/140‑1 (MQ=255)

                                                                                                  cccccGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGTTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                    <  2:1346285/138‑1 (MQ=255)

                                                                                                   ccccGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCta                                   <  1:597654/139‑1 (MQ=255)

                                                                                                   ccccGCACGCTGAACGTGAAGCAAAACTACGAAGAGTGTTTTTCGTTCGTCGGTGAACAGGATCTGCCGCGGGCCGATCTCGATGTGATCCTGATGCGAAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCta                                   <  2:1287319/139‑1 (MQ=255)

                                                                                                       gCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgc                                         <  1:309895/130‑1 (MQ=255)

                                                                                                       gCACGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgc                                         >  2:309895/1‑130 (MQ=255)

                                                                                                         aCGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCt                             >  2:262889/1‑140 (MQ=255)

                                                                                                         aCGCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCt                             >  2:110578/1‑140 (MQ=255)

                                                                                                           gCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTgg                           >  1:157928/1‑140 (MQ=255)

                                                                                                           gCTGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTgg                           >  2:261971/1‑140 (MQ=255)

                                                                                                             tGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGaa                         >  2:406497/1‑140 (MQ=255)

                                                                                                             tGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGaa                         >  1:1146701/1‑140 (MQ=255)

                                                                                                             tGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGa                          >  1:103511/1‑139 (MQ=255)

                                                                                                             tGAACGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGa                          <  2:103511/139‑1 (MQ=255)

                                                                                                               aaCGTGAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACg                       >  1:1124056/1‑140 (MQ=255)

                                                                                                                    gAAGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCg                  <  1:901947/140‑1 (MQ=255)

                                                                                                                     aaGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGa                 >  1:1135048/1‑140 (MQ=255)

                                                                                                                      aGCAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGaa                <  2:1135048/140‑1 (MQ=255)

                                                                                                                        cAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAaga              <  2:57817/140‑1 (MQ=255)

                                                                                                                        cAGAACTACGAAGAGTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGAAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAaga              <  2:1065101/140‑1 (MQ=255)

                                                                                                                              tACGAAAAGTGTTTTTGGTGAGTGGGTAAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGCCCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAgg        <  2:550268/140‑1 (MQ=255)

                                                                                                                                   agagTGATTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGc   <  1:992222/140‑1 (MQ=255)

                                                                                                                                    gagTGATTTTCGTTCGTGGGTGAACAGGATCGGCCGCGGGCCGATCTCGAGGTGGTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCt  <  2:1224513/140‑1 (MQ=255)

AGTTTTGCTATGTTGCTGGAAGCACAGCGTCGTGGTTACGAACTTCACTATATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCT  >  NC_000913/3091938‑3092209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`