Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,127,002	C→T	A340A (GCG→GCA)	glcD ←	glycolate dehydrogenase, putative FAD‑linked subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,127,002	0	C	T	100.0%	258.3 / NA	73	A340A (GCG→GCA)	glcD	glycolate dehydrogenase, putative FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/35);  total (38/35)

TGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACGCCGTCCA  >  NC_000913/3126875‑3127138                                                                                                                                |                                                                                                                                         tGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGa                                                                                                                              >  1:375848/1‑140 (MQ=255)  ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGAc                                                                                                                             <  1:678884/140‑1 (MQ=255)  ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGAc                                                                                                                             <  1:405952/140‑1 (MQ=255)  ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGAc                                                                                                                             >  1:777462/1‑140 (MQ=255)  ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGAc                                                                                                                             >  1:251210/1‑140 (MQ=255)   gCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGAcc                                                                                                                            >  2:87104/1‑140 (MQ=255)          gTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAg                                                                                                                     <  2:506461/140‑1 (MQ=255)           tAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGa                                                                                                                    >  1:970202/1‑140 (MQ=255)              aTCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGAc                                                                                                                             <  2:804530/128‑1 (MQ=255)              aTCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGAc                                                                                                                             >  1:804530/1‑128 (MQ=255)              aTCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAAc                                                                                                                 >  2:1122557/1‑140 (MQ=255)                 atatTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTa                                                                                                              >  2:1114861/1‑140 (MQ=255)                    ttGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGTGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTAcgc                                                                                                           >  2:1084062/1‑140 (MQ=255)                       ctgcGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGct                                                                                                        <  1:589877/140‑1 (MQ=255)                         gcGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGctct                                                                                                      >  1:771858/1‑140 (MQ=255)                          cGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTg                                                                                                     >  1:875281/1‑140 (MQ=255)                          cGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTg                                                                                                     >  1:96136/1‑140 (MQ=255)                          cGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTg                                                                                                     >  2:145082/1‑140 (MQ=255)                            aTAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGTGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCt                                                                                                   <  1:1084062/140‑1 (MQ=255)                            aTAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCt                                                                                                   <  2:875281/140‑1 (MQ=255)                               aaCGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCg                                                                                                >  2:980929/1‑140 (MQ=255)                                 cGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTc                                                                                              <  2:970202/140‑1 (MQ=255)                                   ggCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCt                                                                                            <  2:777462/140‑1 (MQ=255)                                    gCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCtg                                                                                           >  2:123823/1‑140 (MQ=255)                                             tCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgacg                                                                                  <  1:1114861/140‑1 (MQ=255)                                              ccAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTc                                                                                              <  2:340161/127‑1 (MQ=255)                                              ccAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTc                                                                                              >  1:340161/1‑127 (MQ=255)                                              ccAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgacgg                                                                                 >  1:310697/1‑140 (MQ=255)                                                  tACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCGAGACGGACGt                                                                             >  2:1177823/1‑140 (MQ=255)                                                    cGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTATTAATCCGGGGAGATACGTGCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCAGTGCCAGACGGACGTCa                                                                           >  2:1212778/1‑140 (MQ=255)                                                    cGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGcc                                                                                       <  1:569952/128‑1 (MQ=255)                                                    cGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGcc                                                                                       >  2:569952/1‑128 (MQ=255)                                                    cGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCa                                                                           >  2:89808/1‑140 (MQ=255)                                                    cGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCa                                                                           >  2:141333/1‑140 (MQ=255)                                                         ggcaggGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTTCGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTcgc                                                                      >  2:480119/1‑140 (MQ=255)                                                            aggGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGcc                                                                   <  1:93492/140‑1 (MQ=255)                                                             gggCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCC‑GGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCg                                                                 <  1:407120/140‑1 (MQ=255)                                                              ggCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCg                                                                 <  2:310697/140‑1 (MQ=255)                                                              ggCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCg                                                                 <  1:1177823/140‑1 (MQ=255)                                                              ggCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCg                                                                 >  2:494708/1‑140 (MQ=255)                                                              ggCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCg                                                                 >  2:1020650/1‑140 (MQ=255)                                                               gcgcGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCgcgc                                                                    >  2:915434/1‑136 (MQ=255)                                                               gcgcGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCgcgc                                                                    <  1:915434/136‑1 (MQ=255)                                                                         ggATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTTCGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAg                                                      <  1:480119/140‑1 (MQ=255)                                                                         ggATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAg                                                      <  2:375848/140‑1 (MQ=255)                                                                           aTGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGAt                                                    <  1:89808/140‑1 (MQ=255)                                                                           aTGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGAt                                                    <  1:980929/140‑1 (MQ=255)                                                                                    tccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAc                                           >  1:164986/1‑140 (MQ=255)                                                                                    tccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAc                                           >  1:972703/1‑140 (MQ=255)                                                                                     ccatGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAAcc                                          <  2:771858/140‑1 (MQ=255)                                                                                           agtagtAATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCg                                    <  1:123823/140‑1 (MQ=255)                                                                                             tagtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCa                                  <  1:145082/140‑1 (MQ=255)                                                                                             tagtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCa                                  <  2:881290/140‑1 (MQ=255)                                                                                             tagtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCa                                  <  2:1041403/140‑1 (MQ=255)                                                                                              agtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAg                                 <  1:1020650/140‑1 (MQ=255)                                                                                              agtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAg                                 <  2:972703/140‑1 (MQ=255)                                                                                              agtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAg                                 >  1:572615/1‑140 (MQ=255)                                                                                               gtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTaa                                            <  2:23376/128‑1 (MQ=255)                                                                                               gtaATCCGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTaa                                            >  1:23376/1‑128 (MQ=255)                                                                                                     cGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCg                                    >  2:1101576/1‑130 (MQ=255)                                                                                                     cGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCg                                    <  1:1101576/130‑1 (MQ=255)                                                                                                     cGGGGAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCt                          >  1:199819/1‑140 (MQ=255)                                                                                                       gggAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAg                                 <  1:741684/131‑1 (MQ=255)                                                                                                       gggAGATACGTCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAg                                 >  2:741684/1‑131 (MQ=255)                                                                                                                 tCCTACCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACt              >  1:381161/1‑140 (MQ=255)                                                                                                                     aCCGCCGGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCAc          >  2:954585/1‑140 (MQ=255)                                                                                                                      ccgccgGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACg         <  1:954585/140‑1 (MQ=255)                                                                                                                      ccgccgGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACg         >  2:1299724/1‑140 (MQ=255)                                                                                                                         ccgGGAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACGCCg      >  1:1118697/1‑140 (MQ=255)                                                                                                                            ggAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACg         <  2:549739/134‑1 (MQ=255)                                                                                                                            ggAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACg         >  1:549739/1‑134 (MQ=255)                                                                                                                             gAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACGCCGTCCa  <  1:141333/140‑1 (MQ=255)                                                                                                                             gAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACGCCGTCCa  <  1:1212778/140‑1 (MQ=255)                                                                                                                             gAATGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGACGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACGCCGTCCa  <  2:164986/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                         TGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGTCGCGCCCGCTTTCAACAAGATGTCGTTAACCCGCTCGCAGTCTTCCTGTACGTCAGACTCCACGCCGTCCA  >  NC_000913/3126875‑3127138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑