Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,193,821	A→T	I320F (ATC→TTC)	yqiK →	protein YqiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,193,821	0	A	T	100.0%	155.8 / NA	46	I320F (ATC→TTC)	yqiK	protein YqiK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/26);  total (20/26)

TGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGATCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAGCGCCCAACAAAAC  >  NC_000913/3193685‑3193955                                                                                                                                         |                                                                                                                                       tGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCg                                                                                                                                     <  2:224350/140‑1 (MQ=255)   aaGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGaa                                                                                                                                   <  1:1098189/140‑1 (MQ=255)    aGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAAc                                                                                                                                  <  1:119880/140‑1 (MQ=255)    aGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAAc                                                                                                                                  <  1:411682/140‑1 (MQ=255)         aaCGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAAcagcag                                                                                                                             <  1:1129293/140‑1 (MQ=255)         aaCGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAAcagcag                                                                                                                             <  1:204878/140‑1 (MQ=255)                         agagcagaCACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCg                                                                                                             <  2:790065/140‑1 (MQ=255)                          gagcagaCACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGc                                                                                                            <  1:712743/140‑1 (MQ=255)                          gagcagaCACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGc                                                                                                            <  2:830552/140‑1 (MQ=255)                           agcagaCACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCt                                                                                                           >  2:228692/1‑140 (MQ=255)                           agcagaCACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCt                                                                                                           >  1:566928/1‑140 (MQ=255)                               gaCACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAAcc                                                                                                       >  1:488496/1‑140 (MQ=255)                                acacGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCa                                                                                                      >  2:270070/1‑140 (MQ=255)                                acacGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCa                                                                                                      >  2:148847/1‑140 (MQ=255)                                  acGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGa                                                                                                    >  1:260300/1‑140 (MQ=255)                                     aaTTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAg                                                                                                     >  1:1108381/1‑136 (MQ=255)                                     aaTTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAg                                                                                                     <  2:1108381/136‑1 (MQ=255)                                            gCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAatcgat                                                                                          >  1:721503/1‑140 (MQ=255)                                              tGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAatcgatcg                                                                                        <  2:1230390/140‑1 (MQ=255)                                                aaCGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCt                                                                                      >  2:1265349/1‑140 (MQ=255)                                                          tCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGCGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCa                                                                            >  1:1207797/1‑140 (MQ=255)                                                               aaaCAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCg                                                                       >  2:1288942/1‑140 (MQ=255)                                                                aaCAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCgg                                                                      <  2:721503/140‑1 (MQ=255)                                                                  cAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGaa                                                                    >  2:676434/1‑140 (MQ=255)                                                                     aaaTCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGaacaa                                                                 <  2:566928/140‑1 (MQ=255)                                                                            ccGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTccc                                                          <  2:1096300/140‑1 (MQ=255)                                                                             cgcgAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAacaaca                                                               <  2:735034/134‑1 (MQ=255)                                                                             cgcgAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAacaaca                                                               >  1:735034/1‑134 (MQ=255)                                                                                      gCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAgc                                                <  1:270070/140‑1 (MQ=255)                                                                                       ccGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAgcg                                               <  2:130713/140‑1 (MQ=255)                                                                                       ccGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAgcg                                               <  1:59056/140‑1 (MQ=255)                                                                                       ccGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAgcg                                               <  2:260300/140‑1 (MQ=255)                                                                                          tCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGGTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGt                                            >  2:1263742/1‑140 (MQ=255)                                                                                           ccGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTg                                           <  1:640106/140‑1 (MQ=255)                                                                                             gCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCt                                         >  2:110588/1‑140 (MQ=255)                                                                                                  aGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAAtct                                    <  1:676434/140‑1 (MQ=255)                                                                                                     aaGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGc                                 <  2:704487/140‑1 (MQ=255)                                                                                                        gTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACt                              >  2:1102180/1‑140 (MQ=255)                                                                                                           gAAGCTGAACGTGAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGc                           >  2:846970/1‑140 (MQ=255)                                                                                                                       gAAGTTCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAg               >  2:1058552/1‑140 (MQ=255)                                                                                                                       gAAGTTCGCATTAAAGAGTACGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAg               <  2:805460/140‑1 (MQ=255)                                                                                                                            tCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAGCGccc          >  2:1299406/1‑140 (MQ=255)                                                                                                                            tCGCATTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAGCGccc          >  1:1206079/1‑140 (MQ=255)                                                                                                                                aTTAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAGCGCCcaaca      <  1:1299406/140‑1 (MQ=255)                                                                                                                                 ttAAAGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAGCGCCcaacaa     <  1:846970/140‑1 (MQ=255)                                                                                                                                    aaGAGTTCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAGCGCCCAACAAAAc  <  1:1102180/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TGAAGCTGAACGTCGTCGTGAAGCAGAGCAGACACGAATTCTGGCTGAACGACAGATTCAGGAAACAGAAATCGACCGCGAACAGGCCGTCCGCTCAAGAAAGGTTGAAGCTGAACGTGAAGTTCGCATTAAAGAGATCGAACAGCAGCAGGTCACCGAAATCGCTAACCAGACGAAATCGATCGCTATTGCCGCCAAATCGGAACAACAGTCCCAGGCAGAAGCGCGTGCTAATCTCGCACTTGCAGAAGCGGTAAGCGCCCAACAAAAC  >  NC_000913/3193685‑3193955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑