Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,210,638	T→C	intergenic (‑95/‑143)	tsaD ← / → rpsU	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit/30S ribosomal subunit protein S21

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,210,638	0	T	C	100.0%	221.4 / NA	62	intergenic (‑95/‑143)	tsaD/rpsU	N(6)‑L‑threonylcarbamoyladenine synthase, TsaD subunit/30S ribosomal subunit protein S21
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/31);  total (31/31)

TGCCGGTTTCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACTCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGA  >  NC_000913/3210501‑3210773                                                                                                                                          |                                                                                                                                        tGCCGGTTTCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATAcccc                                                                                                                                       >  2:286483/1‑140 (MQ=255)  gCCGGTTTCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATAcccct                                                                                                                                      <  2:791892/140‑1 (MQ=255)   ccGGATTCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATAcccctc                                                                                                                                     <  2:1251620/140‑1 (MQ=255)    cGGTTTCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCa                                                                                                                                    <  1:1339423/140‑1 (MQ=255)       tttCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCAccc                                                                                                                                 <  1:286483/140‑1 (MQ=255)       tttCATCGCAGGAAGTTACAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCAccc                                                                                                                                 <  2:433/140‑1 (MQ=255)        ttCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCt                                                                                                                                <  2:7879/140‑1 (MQ=255)            tCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTtata                                                                                                                            >  1:778666/1‑140 (MQ=255)             cGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATaa                                                                                                                           >  2:115619/1‑140 (MQ=255)                               ccAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAg                                                                                                         >  1:900530/1‑140 (MQ=255)                                cAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAgg                                                                                                        <  1:47987/140‑1 (MQ=255)                                cAGCACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAGCTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAgg                                                                                                        >  2:926360/1‑140 (MQ=255)                                   tACACGCATGACTTGTTTTACCTCGCTTTATTGCCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     <  1:90532/140‑1 (MQ=255)                                   tACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     >  1:1087089/1‑140 (MQ=255)                                   tACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     >  2:989256/1‑140 (MQ=255)                                   tACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     <  2:631475/140‑1 (MQ=255)                                     cacGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcgcg                                                                                                   <  2:449024/140‑1 (MQ=255)                                     cacGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcgcg                                                                                                   <  1:419875/140‑1 (MQ=255)                                      acGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCgg                                                                                                  >  2:38642/1‑140 (MQ=255)                                        gCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     >  2:952416/1‑135 (MQ=255)                                        gCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTg                                                                                                <  1:140656/140‑1 (MQ=255)                                        gCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTg                                                                                                >  1:849773/1‑140 (MQ=255)                                        gCATGACTTGTTTTACCTCGCATTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     <  1:952416/135‑1 (MQ=255)                                             aCTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     >  2:418351/1‑130 (MQ=255)                                             aCTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCcg                                                                                                     <  1:418351/130‑1 (MQ=255)                                             aCTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTa                                                                                           >  2:223200/1‑140 (MQ=255)                                             aCTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTa                                                                                           >  2:1019133/1‑140 (MQ=255)                                               ttGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACa                                                                                         <  1:1019133/140‑1 (MQ=255)                                                tGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACaa                                                                                        >  1:233712/1‑140 (MQ=255)                                                  ttttACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAag                                                                                      <  2:849773/140‑1 (MQ=255)                                                         tCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAagcagcagc                                                                               <  2:778666/140‑1 (MQ=255)                                                            cTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAAt                                                                            <  2:1087089/140‑1 (MQ=255)                                                                       cgcAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAAt                                                                 >  1:1185610/1‑140 (MQ=255)                                                                         cAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTc                                                               >  2:663864/1‑140 (MQ=255)                                                                            tgtAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGc                                                            >  2:25821/1‑140 (MQ=255)                                                                              tAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCAc                                                          >  1:226854/1‑140 (MQ=255)                                                                              tAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCAc                                                          <  1:25821/140‑1 (MQ=255)                                                                                  aCCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCAtt                                                      <  1:1289574/140‑1 (MQ=255)                                                                                  aCCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCAtt                                                      <  1:663864/140‑1 (MQ=255)                                                                                    cAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCAtttt                                                    >  2:865162/1‑140 (MQ=255)                                                                                      aTGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGa                                                  >  1:1363083/1‑140 (MQ=255)                                                                                      aTGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGa                                                  >  2:976265/1‑140 (MQ=255)                                                                                      aTGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGa                                                  >  2:326411/1‑140 (MQ=255)                                                                                      aTGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGa                                                  >  1:1346289/1‑140 (MQ=255)                                                                                      aTGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGa                                                  >  2:52466/1‑140 (MQ=255)                                                                                      aTGCGGGTTGATGTAAAACTTTGTTCG‑CCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGaa                                                 >  2:909661/1‑140 (MQ=255)                                                                                       tGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGaa                                                 <  1:223200/140‑1 (MQ=255)                                                                                               gATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGGTg                                         <  1:497700/140‑1 (MQ=255)                                                                                                 tGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGc                                       >  1:45634/1‑140 (MQ=255)                                                                                                  gTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCg                                      <  2:1346289/140‑1 (MQ=255)                                                                                                   tAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGt                                     >  1:1097730/1‑140 (MQ=255)                                                                                                            gTTCGCCCCTGGAGAAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAg                            >  1:1134532/1‑140 (MQ=255)                                                                                                                        agaAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGaa                <  1:865162/140‑1 (MQ=255)                                                                                                                        agaAAGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGaa                <  1:52466/140‑1 (MQ=255)                                                                                                                          aaaGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAAtt              <  2:1134532/140‑1 (MQ=255)                                                                                                                            aGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGaa                >  2:818961/1‑136 (MQ=255)                                                                                                                            aGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGaa                <  1:818961/136‑1 (MQ=255)                                                                                                                            aGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAAt               <  2:261872/137‑1 (MQ=255)                                                                                                                            aGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAAt               >  1:261872/1‑137 (MQ=255)                                                                                                                            aGCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTaa            >  2:1149334/1‑140 (MQ=255)                                                                                                                             gCCTCGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAAt           <  1:326411/140‑1 (MQ=255)                                                                                                                                 cGTGTATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCaaa       >  2:954335/1‑140 (MQ=255)                                                                                                                                  gtgtATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAg      <  1:954335/140‑1 (MQ=255)                                                                                                                                    gtATACCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGt    <  1:38642/140‑1 (MQ=255)                                                                                                                                      ataCCCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTga  <  1:115619/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGCCGGTTTCATCGCAGGAAGTTTCAATACCCAGTACACGCATGACTTGTTTTACCTCGCTTTATTACCGCGCAGTGTAGGACCAATGCGGGTTGATGTAAAACTTTGTTCGCCCCTGGAGAAAGCCTCGTGTATACTCCTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGCAATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGA  >  NC_000913/3210501‑3210773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑