Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,211,354	T→C	V83A (GTC→GCC)	dnaG →	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,211,354	0	T	C	98.6%	248.2 / ‑4.5	72	V83A (GTC→GCC)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (31/40);  total (31/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

GCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTAC  >  NC_000913/3211218‑3211487                                                                                                                                         |                                                                                                                                      gcgTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGa                                                                                                                                    >  1:361119/1‑140 (MQ=255)   gtgtTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGaaa                                                                                                                                  <  1:919607/140‑1 (MQ=255)    tgttgtCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAAc                                                                                                                                 >  1:1206878/1‑140 (MQ=255)      ttgtCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACgg                                                                                                                               <  1:1290880/140‑1 (MQ=255)           cATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGaa                                                                                                                          <  1:871140/140‑1 (MQ=255)               ccACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGc                                                                                                                      >  2:145960/1‑140 (MQ=255)                cacaACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCt                                                                                                                     >  2:1346916/1‑140 (MQ=255)                  caACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTgg                                                                                                                   <  2:361119/140‑1 (MQ=255)                   aaCGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGgc                                                                                                                  <  2:1001219/140‑1 (MQ=255)                   aaCGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGgc                                                                                                                  >  1:6614/1‑140 (MQ=255)                     cGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGgcag                                                                                                                <  2:492572/140‑1 (MQ=255)                     cGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGgcag                                                                                                                >  2:1134268/1‑140 (MQ=255)                      gagaAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGgcagc                                                                                                               <  2:6614/140‑1 (MQ=255)                        gaAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCaa                                                                                                             >  2:43293/1‑140 (MQ=255)                          aaaCCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATg                                                                                                           <  1:374706/140‑1 (MQ=255)                            aCCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGca                                                                                                         <  1:1134268/140‑1 (MQ=255)                            aCCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGca                                                                                                         <  2:1110800/140‑1 (MQ=255)                            aCCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGca                                                                                                         <  1:560542/140‑1 (MQ=255)                                         ccGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTg                                                                                            <  2:827384/140‑1 (MQ=255)                                         ccGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCAAAGAGCTGGCAGCAATGCACAATCTTGAAGTg                                                                                            <  2:349101/140‑1 (MQ=255)                                            ttAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCa                                                                                         >  1:243137/1‑140 (MQ=255)                                            ttAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCa                                                                                         >  1:620433/1‑140 (MQ=255)                                             tAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCAt                                                                                        <  2:1069121/140‑1 (MQ=255)                                              aaCGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGca                                                                                                         >  1:1084728/1‑122 (MQ=255)                                              aaCGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGca                                                                                                         <  2:1084728/122‑1 (MQ=255)                                               aCGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCAttt                                                                                      >  2:1177725/1‑140 (MQ=255)                                                    gagaAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAgc                                                                                 >  2:214335/1‑140 (MQ=255)                                                    gagaAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAgc                                                                                 >  2:1116498/1‑140 (MQ=255)                                                     agaAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAgca                                                                                >  2:383561/1‑140 (MQ=255)                                                          cAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAgcaggcag                                                                           <  2:248917/140‑1 (MQ=255)                                                             ttttACCACTGCTTTGGATGTGGCGCGCACGGCAACGC‑ATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGc                                                                       <  2:60246/140‑1 (MQ=255)                                                              tttACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCTCAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGc                                                                       <  2:1206878/140‑1 (MQ=255)                                                              tttACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGc                                                                       <  1:687717/140‑1 (MQ=255)                                                                tACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGccc                                                                     <  2:620433/140‑1 (MQ=255)                                                                     cTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGc                                                                       <  2:898943/133‑1 (MQ=255)                                                                     cTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGc                                                                       >  1:898943/1‑133 (MQ=255)                                                                              aTGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAgc                                                       <  1:118114/140‑1 (MQ=255)                                                                               tgtgGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAgcg                                                      <  2:1316824/140‑1 (MQ=255)                                                                                     gcgcACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCa                                                <  2:729341/140‑1 (MQ=255)                                                                                              aaCGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAAc                                       <  1:1177725/140‑1 (MQ=255)                                                                                                 gcgATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCt                                    <  2:743488/140‑1 (MQ=255)                                                                                                 gcgATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCt                                    >  2:27916/1‑140 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTAt                                <  1:383561/140‑1 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTAt                                <  1:1116498/140‑1 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTAt                                >  2:108447/1‑140 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTAt                                >  2:479602/1‑140 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTAt                                <  1:1057617/140‑1 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCAAGCGCCATCAGAGGCAAACGCTTTAt                                >  2:778309/1‑140 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGACCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCAt                                                  <  2:747882/122‑1 (MQ=255)                                                                                                     tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGACCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCAt                                                  >  1:747882/1‑122 (MQ=255)                                                                                                               gatgaACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATgg                      >  2:1278143/1‑140 (MQ=255)                                                                                                               gatgaACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATgg                      >  2:810420/1‑140 (MQ=255)                                                                                                                atgaACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                     <  1:1157888/140‑1 (MQ=255)                                                                                                                atgaACTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                     <  1:145960/140‑1 (MQ=255)                                                                                                                   aaCTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAg                             <  2:261205/129‑1 (MQ=255)                                                                                                                   aaCTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAg                             >  1:261205/1‑129 (MQ=255)                                                                                                                    aCTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGt                 <  1:108447/140‑1 (MQ=255)                                                                                                                    aCTACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGt                 <  1:83256/140‑1 (MQ=255)                                                                                                                      tACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTg                          <  1:922233/129‑1 (MQ=255)                                                                                                                      tACGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTg                          >  2:922233/1‑129 (MQ=255)                                                                                                                       aCGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGAc                    <  2:1272507/134‑1 (MQ=255)                                                                                                                       aCGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGAc                    >  1:1272507/1‑134 (MQ=255)                                                                                                                       aCGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTg              >  2:1321029/1‑140 (MQ=255)                                                                                                                        cGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                     >  2:408287/1‑132 (MQ=255)                                                                                                                        cGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                     <  1:91438/132‑1 (MQ=255)                                                                                                                        cGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                     <  1:408287/132‑1 (MQ=255)                                                                                                                        cGACAAGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                     >  2:91438/1‑132 (MQ=255)                                                                                                                             aGCTCGAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACg        >  1:967135/1‑140 (MQ=255)                                                                                                                                  gAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACgg                  <  2:1103246/125‑1 (MQ=255)                                                                                                                                  gAGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACgg                  >  1:1103246/1‑125 (MQ=255)                                                                                                                                   aGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTAc  >  2:128424/1‑140 (MQ=255)                                                                                                                                   aGTTCGCCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTAc  <  2:969966/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTAC  >  NC_000913/3211218‑3211487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑