breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 21 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913730,5370CT68.2% 4.7 / 14.9 22R319C (CGC→TGC) rhsCrhs element protein RhsC
*NC_00091329,4960TA47.5% 32.9 / 34.6 40intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_0009134,604,3440CT40.6% 62.5 / 37.8 64S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009131,825,0610GA37.5% 37.6 / 14.7 32intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009132,001,9180AC34.0% 76.7 / 22.3 50intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009131,270,0090TA32.2% 134.5 / 92.0 90intergenic (+86/+229)rdlB/ldrCsmall regulatory antisense RNA RdlB/small toxic polypeptide LdrC
*NC_0009134,101,5140GT31.7% 91.7 / 20.3 41intergenic (+84/‑176)sodA/kdgTsuperoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
*NC_0009133,982,6761.C28.8% 89.4 / 75.6 59noncoding (71/87 nt)leuTtRNA‑Leu
*NC_000913650,8090TG27.1% 98.2 / 12.4 48T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_000913332,9910GT26.7% 110.1 / 17.2 45L207F (TTG→TTTpdeLDNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
*NC_0009133,084,5260CT25.3% 160.5 / 55.2 91Q462Q (CAG→CAAspeAarginine decarboxylase, biosynthetic
*NC_0009132,326,4500TG25.0% 81.2 / 15.7 40G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_000913372,6400TG24.6% 136.6 / 14.3 61L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
*NC_0009131,645,0340AC23.5% 104.9 / 13.6 51intergenic (‑132/+85)rem/hokDQin prophage; protein Rem/Qin prophage; toxic protein HokD
*NC_0009134,098,1550AC23.5% 150.0 / 12.2 69L140F (TTA→TTCrhaSDNA‑binding transcriptional activator RhaS
*NC_0009131,491,0980AC23.2% 154.9 / 11.8 69Y66S (TAC→TCC) cybBcytochrome b561
*NC_0009134,287,7190TA23.1% 156.0 / 20.2 65intergenic (‑348/‑45)acs/nrfAacetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase
*NC_0009133,740,9090TG22.9% 141.2 / 10.9 61V402G (GTG→GGG) avtAvaline‑‑pyruvate aminotransferase
*NC_0009133,926,3660AC22.3% 219.1 / 19.5 94G30G (GGT→GGGmioCflavoprotein MioC
*NC_0009131,577,1230AC21.1% 224.9 / 15.2 90V166G (GTA→GGA) yddBputative TonB‑dependent receptor

Marginal new junction evidence (lowest skew 10 of 27 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)14 (0.270) 9/188 3.6 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 374922 =NA (NA)23 (0.410) 10/200 3.6 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375059NA (NA)27 (0.360)
+T
14/272 3.8 NA noncoding (28/34 nt) other REP31c
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 375102 =NA (NA)20 (0.330) 9/214 4.1 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 454269056 (0.730)11 (0.150) 10/256 4.6 15.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 69 (0.960)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 375001 =NA (NA)9 (0.150) 7/214 4.8 NA noncoding (17/36 nt) other REP31b
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 4542682 =65 (0.850)8 (0.110) 8/256 5.2 11.0% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 69 (0.960)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 375246NA (NA)7 (0.100)
+GAACGCCTTATCCGGT
7/242 5.3 NA noncoding (13/34 nt) other REP31g
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 55583370 (0.910)6 (0.090) 6/242 5.7 6.8% coding (1223/1386 nt) cysS cysteine‑‑tRNA ligase
?NC_000913 = 556784 102 (1.510)coding (90/213 nt) ybcJ putative RNA‑binding protein YbcJ
* ? NC_000913 = 375160NA (NA)9 (0.120)
+T
7/272 5.8 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d