Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,838,357	(G)5→4	coding (1146/1536 nt)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,838,353	0	G	.	100.0%	144.5 / NA	35	coding (1142/1536 nt)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (19/16);  total (19/16)

GGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCTGGGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTGGGCTGGTCGCGGGCCAAAATCTTCTTTTACGTGAAATG  >  NC_000913/1838218‑1838488                                                                                                                                        |                                                                                                                                        ggCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCTggggc                                                                                                                                     >  2:676091/1‑139 (MQ=255) ggCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCTggggc                                                                                                                                     >  1:850279/1‑139 (MQ=255)        aTGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATctgct                                                                                                                             <  1:691400/140‑1 (MQ=255)                 acaGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGAt                                                                                                                    >  2:95078/1‑140 (MQ=255)                     cGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCg                                                                                                                >  1:545715/1‑140 (MQ=255)                     cGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCg                                                                                                                >  1:727643/1‑140 (MQ=255)                       tCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTg                                                                                                              >  2:46988/1‑140 (MQ=255)                        cGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTgg                                                                                                             <  1:46988/140‑1 (MQ=255)                          ccAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGAt                                                                                                           <  1:265863/140‑1 (MQ=255)                                           tGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAAc                                                                                          <  2:545715/140‑1 (MQ=255)                                             cccATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAAccc                                                                                        >  2:560963/1‑140 (MQ=255)                                             cccATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAAccc                                                                                        >  1:732536/1‑140 (MQ=255)                                             cccATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAAcc                                                                                         >  1:275952/1‑139 (MQ=255)                                             cccATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAAcc                                                                                         <  2:275952/139‑1 (MQ=255)                                                   ttattaCCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTgg                                                                                  <  1:397596/140‑1 (MQ=255)                                                     attaCCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCa                                                                                <  2:732536/140‑1 (MQ=255)                                                      ttaCCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAg                                                                               <  2:850279/140‑1 (MQ=255)                                                       taCCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGGCGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAgc                                                                              >  1:374388/1‑140 (MQ=255)                                                       taCCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAgc                                                                              >  2:436787/1‑140 (MQ=255)                                                            gCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCAtt                                                                         >  1:815541/1‑140 (MQ=255)                                                              tctGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGa                                                                       >  2:578412/1‑140 (MQ=255)                                                                 gCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTc                                                                    <  1:578412/140‑1 (MQ=255)                                                                 gCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTc                                                                    <  2:632243/140‑1 (MQ=255)                                                                       ggtggCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGtt                                                              >  2:330442/1‑140 (MQ=255)                                                                        gtggCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTa                                                             >  1:814185/1‑140 (MQ=255)                                                                            cGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAAttt                                                         >  2:629632/1‑140 (MQ=255)                                                                                     acacGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTgcgc                                                <  2:272320/140‑1 (MQ=255)                                                                                     acacGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTgcgc                                                <  2:814185/140‑1 (MQ=255)                                                                                         gCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAac                                            >  2:651015/1‑140 (MQ=255)                                                                                              cgcTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAacac                                          <  2:175199/137‑1 (MQ=255)                                                                                              cgcTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAacac                                          >  1:175199/1‑137 (MQ=255)                                                                                              cgcTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTgg                                       >  2:509291/1‑140 (MQ=255)                                                                                              cgcTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTgg                                       >  1:154506/1‑140 (MQ=255)                                                                                                           aCTGGATGGAAGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTGGGCTGGTCGCGGGc                          <  2:154506/140‑1 (MQ=255)                                                                                                                    aaGCTGGACGGCGGTGGTCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTGGGCTGGTCGCGGGCCAAAAtctt                 >  1:330149/1‑140 (MQ=255)                                                                                                                                  tggtCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTGGGCTGGTCGCGGGCCAAAATCTTCTTTTACGTGAAAt   <  1:95078/140‑1 (MQ=255)                                                                                                                                   ggtCT‑GGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTGGGCTGGTCGCGGGCCAAAATCTTCTTTTACGTGAAATg  <  2:727643/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        GGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTACACGCTGGCGCTATGGCTGAAACTGGATGGAAGCTGGACGGCGGTGGTCTGGGGGCATCTGCTGTGGGTGATGCCGTGGATGCTGTTTATCCTGCAACCCGCCTGGCAGCGCATTGATTCACGGTTAATTTTGATTGCGCAAACACTGGGCTGGTCGCGGGCCAAAATCTTCTTTTACGTGAAATG  >  NC_000913/1838218‑1838488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑