| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 380,022 | (G)10→11 | intergenic (‑141/+47) | frmR ← / ← yaiO | DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,012 | 1 | . | G | 87.0% | 163.9 / 17.9 | 54 | intergenic (‑131/+57) | frmR/yaiO | DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO |
| Reads supporting (aligned to +/- strand): ref base . (5/2); new base G (30/17); total (35/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
TTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACC > NC_000913/379882‑380146 | ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑ggggggggg < 2:855477/140‑1 (MQ=255) taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAg > 2:285773/1‑140 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCg > 1:162151/1‑132 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCg < 2:162151/132‑1 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAgcgg > 2:548850/1‑140 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAgcgg > 2:668470/1‑140 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAgcg < 1:169882/139‑1 (MQ=255) gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAgcg > 2:169882/1‑139 (MQ=255) ggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGGGAATGTGGGCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAgcggg < 2:789737/140‑1 (MQ=255) ggggATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGAGAgcgggc > 1:181104/1‑140 (MQ=255) atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa < 2:187837/140‑1 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc > 2:7706/1‑140 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc > 1:293862/1‑140 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt > 2:849580/1‑140 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt > 1:17181/1‑140 (MQ=255) taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg < 1:285773/140‑1 (MQ=255) gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg < 1:668470/140‑1 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGGGAAAG‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc > 2:819423/1‑140 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa > 1:534495/1‑133 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa < 2:534495/133‑1 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg > 1:201216/1‑140 (MQ=255) aaaaCAATGGAAAAAAAAATTTATCCGGGGAATGGGGTCGGAAAACAAAGGGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc < 1:489343/138‑1 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa > 2:654342/1‑140 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa > 1:409550/1‑140 (MQ=255) aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCCAAACGGGAAGGGAAGGCCGCCCCGGAAAGCGGGGGGCAAAAGGAATAAAAATTTCCAGGCCAGACGGGCGGCaa > 2:489343/1‑140 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt > 2:336396/1‑140 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt > 2:158093/1‑140 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt > 1:272867/1‑140 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc > 2:810540/1‑140 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc > 1:136709/1‑140 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc > 1:623470/1‑140 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 1:109429/1‑140 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 1:297957/1‑140 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg > 1:246001/1‑140 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg > 2:581527/1‑140 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg > 2:197750/1‑140 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg > 1:370559/1‑140 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg > 1:399868/1‑140 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGtt > 2:173127/1‑140 (MQ=255) tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc < 2:623470/140‑1 (MQ=255) tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc < 1:197750/140‑1 (MQ=255) ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc < 1:121771/137‑1 (MQ=255) ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatc > 2:121771/1‑137 (MQ=255) gAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg < 2:136709/140‑1 (MQ=255) aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg < 1:173127/140‑1 (MQ=255) gtgGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt > 1:538836/1‑140 (MQ=255) tCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GACAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg < 2:658506/134‑1 (MQ=255) tCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GACAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg > 1:658506/1‑134 (MQ=255) tCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGt > 1:253503/1‑140 (MQ=255) ggAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa < 2:293862/140‑1 (MQ=255) gAAAACAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc > 2:506081/1‑140 (MQ=255) aCAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcaca < 2:409550/140‑1 (MQ=255) aCAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcaca < 1:548850/140‑1 (MQ=255) cAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg > 2:303663/1‑126 (MQ=255) cAAAGAGGAAAGGGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg < 1:303663/126‑1 (MQ=255) agGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc < 2:253503/140‑1 (MQ=255) | TTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGGCTAATC‑GGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACC > NC_000913/379882‑380146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |