Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	185,089	(A)7→8	intergenic (‑20/+34)	yaeI ← / ← dapD	phosphodiesterase YaeI/tetrahydrodipicolinate succinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	185,082	1	.	A	100.0%	154.6 / NA	44	intergenic (‑13/+41)	yaeI/dapD	phosphodiesterase YaeI/tetrahydrodipicolinate succinylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (23/21);  total (23/21)

AAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGC‑‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTA  >  NC_000913/184946‑185215                                                                                                                                          |                                                                                                                                       aaaaGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGC‑‑aaa                                                                                                                                    <  2:473069/140‑1 (MQ=255)           gcgGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGAATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCggg                                                                                                                           <  2:801123/140‑1 (MQ=255)                              aaCCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCAAAAAAAAATCGGGCAGCGTCGTGCTGCCCgt                                                                                                         >  2:682750/1‑140 (MQ=255)                                  aGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCa                                                                                                    <  2:401867/140‑1 (MQ=255)                                  aGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCa                                                                                                    <  2:357523/140‑1 (MQ=255)                                    ccTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATa                                                                                                  >  1:163325/1‑140 (MQ=255)                                    ccTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATa                                                                                                  >  2:224190/1‑140 (MQ=255)                                              aGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCg                                                                                                          <  2:805901/122‑1 (MQ=255)                                              aGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCg                                                                                                          >  1:805901/1‑122 (MQ=255)                                              aGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGt                                                                                          <  1:341723/138‑1 (MQ=255)                                              aGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGt                                                                                          >  2:341723/1‑138 (MQ=255)                                              aGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCg                                                                                        >  2:789056/1‑140 (MQ=255)                                                     cATTTAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTAc                                                                                 >  2:95461/1‑140 (MQ=255)                                                      atatAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACg                                                                                <  2:296436/140‑1 (MQ=255)                                                        ataACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGa                                                                                       <  1:219557/131‑1 (MQ=255)                                                        ataACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGa                                                                                       >  2:219557/1‑131 (MQ=255)                                                        ataACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACgca                                                                              >  2:686254/1‑140 (MQ=255)                                                        ataACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACgca                                                                              <  2:125593/140‑1 (MQ=255)                                                          aaCCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACgcagc                                                                            >  1:560876/1‑140 (MQ=255)                                                          aaCCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACgcagc                                                                            >  2:579450/1‑140 (MQ=255)                                                            ccGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGcagcag                                                                          <  2:163325/140‑1 (MQ=255)                                                             cGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCTTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGt                                                                         >  2:392525/1‑140 (MQ=255)                                                             cGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGa                                                                                       >  2:454401/1‑126 (MQ=255)                                                             cGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGa                                                                                       <  1:454401/126‑1 (MQ=255)                                                                                  tcgtcgCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCcg                                                    >  2:506789/1‑140 (MQ=255)                                                                                  tcgtcgCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCcg                                                    >  2:642328/1‑140 (MQ=255)                                                                                          gCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGtttt                                            <  1:224190/140‑1 (MQ=255)                                                                                            aGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTcg                                          >  1:797577/1‑140 (MQ=255)                                                                                                gCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCGGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTc                                      >  2:759102/1‑140 (MQ=255)                                                                                                     cAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACtt                                 >  1:390623/1‑140 (MQ=255)                                                                                                         aaTCGGCGGCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGAAGATCGTTAATGCCGACTTTGCCGCGAGTCTTCGCGTCAACTTTCtt                             <  1:759102/140‑1 (MQ=255)                                                                                                            cggcggCGTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGAGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAAc                          <  1:642328/140‑1 (MQ=255)                                                                                                               cggcgTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGAt                       >  1:175637/1‑140 (MQ=255)                                                                                                                 gcgTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATaa                     <  1:506789/140‑1 (MQ=255)                                                                                                                 gcgTGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATaa                     <  1:253949/140‑1 (MQ=255)                                                                                                                    tGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCg                  <  1:579450/140‑1 (MQ=255)                                                                                                                    tGAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCg                  <  1:28667/140‑1 (MQ=255)                                                                                                                     gAAATCATCGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGc                 <  1:652182/140‑1 (MQ=255)                                                                                                                           atcGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTa           <  1:95461/140‑1 (MQ=255)                                                                                                                            tcGCTCATCCCTGCAAAAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTa           <  1:682750/140‑1 (MQ=255)                                                                                                                            tcGCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTag          >  2:345348/1‑140 (MQ=255)                                                                                                                              gCTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTagag        >  2:216347/1‑140 (MQ=255)                                                                                                                               cTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAgg       <  1:686254/140‑1 (MQ=255)                                                                                                                               cTCATCCCTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAgg       >  1:19003/1‑140 (MQ=255)                                                                                                                                    cccTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTa  >  2:201081/1‑140 (MQ=255)                                                                                                                                    cccTGCA‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTa  >  2:772985/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AAAAGGCGAGGCGGTGACGGATTAATTCAAACCAGCCTGGCTCACAGTAATGCATATAACCGAAACCTGAGCTCGTGGCTATCGTCGCGGCAGTAGCCTGCAAAAATCGGCGGCGTGAAATCATCGCTCATCCCTGC‑‑AAAAAAATCGGGCAGCGTCGTGCTGCCCGTGTGCATACTTTTAGTCGATGGTACGCAGCAGTTCGTTAATGCCGACTTTGCCGCGAGTTTTCGCGTCAACTTTCTTAACGATAACCGCACAGTAGAGGCTGTA  >  NC_000913/184946‑185215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑