Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,547	(T)5→6	intergenic (‑75/‑361)	mipA ← / → yeaG	scaffolding protein that interacts with murein polymerase and murein hydrolase/protein kinase YeaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,542	1	.	T	100.0%	145.4 / NA	41	intergenic (‑70/‑366)	mipA/yeaG	scaffolding protein that interacts with murein polymerase and murein hydrolase/protein kinase YeaG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (19/22);  total (19/22)

AGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACC‑TTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGATGAG  >  NC_000913/1866407‑1866675                                                                                                                                         |                                                                                                                                      aGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACC‑tttt                                                                                                                                   >  2:538534/1‑140 (MQ=255)  gCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACC‑ttttt                                                                                                                                  >  2:141435/1‑140 (MQ=255)  gCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACC‑ttttt                                                                                                                                  >  2:222231/1‑140 (MQ=255)                  cAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCAtttttt                                                                                                                   >  1:749583/1‑140 (MQ=255)                       gTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGt                                                                                                              <  2:143375/140‑1 (MQ=255)                        ttGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTa                                                                                                             <  2:749583/140‑1 (MQ=255)                         tGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTaa                                                                                                            >  1:161934/1‑140 (MQ=255)                         tGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTaa                                                                                                            >  1:138617/1‑140 (MQ=255)                         tGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTaa                                                                                                            >  2:810425/1‑140 (MQ=255)                                aGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTa                                                                                                     >  2:208331/1‑140 (MQ=255)                                          gTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGa                                                                                           >  1:404463/1‑140 (MQ=255)                                          gTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGa                                                                                           >  1:486964/1‑140 (MQ=255)                                           tGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTGGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGAc                                                                                          <  1:437685/140‑1 (MQ=255)                                           tGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGAc                                                                                          <  2:404463/140‑1 (MQ=255)                                           tGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGAc                                                                                          <  1:141435/140‑1 (MQ=255)                                                      tgagtttgGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGacac                                                                               >  1:370589/1‑140 (MQ=255)                                                                        aTCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATg                                                             >  2:851514/1‑140 (MQ=255)                                                                        aTCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATAAATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTa                                                                       >  2:544739/1‑130 (MQ=255)                                                                        aTCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATAAATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTa                                                                       <  1:544739/130‑1 (MQ=255)                                                                             tCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAAttt                                                        >  1:594565/1‑140 (MQ=255)                                                                               cTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAg                                                      >  2:367718/1‑140 (MQ=255)                                                                                 tAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCg                                                    >  1:400936/1‑140 (MQ=255)                                                                                        aTGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTgg                                             <  2:370589/140‑1 (MQ=255)                                                                                        aTGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTgg                                             <  2:161934/140‑1 (MQ=255)                                                                                        aTGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTgg                                             <  1:725167/140‑1 (MQ=255)                                                                                             tAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAAtt                                                         >  2:583445/1‑123 (MQ=255)                                                                                             tAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAAtt                                                         <  1:583445/123‑1 (MQ=255)                                                                                             tAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGc                                        <  1:804288/140‑1 (MQ=255)                                                                                             tAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGc                                        <  1:367718/140‑1 (MQ=255)                                                                                                  ggCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGcc                                       >  1:847217/1‑136 (MQ=255)                                                                                                  ggCGACAAAGTTTACCCGTCAATACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGcc                                       <  2:847217/136‑1 (MQ=255)                                                                                                                      aaTACATCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCaaa               <  1:538534/140‑1 (MQ=255)                                                                                                                           aTCGAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTa          <  2:486964/140‑1 (MQ=255)                                                                                                                              gAAACCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGAGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATgg       >  2:258079/1‑140 (MQ=255)                                                                                                                               aaaCCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGa      <  2:453824/140‑1 (MQ=255)                                                                                                                               aaaCCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGa      >  1:453824/1‑140 (MQ=255)                                                                                                                               aaaCCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGa      <  1:851514/140‑1 (MQ=255)                                                                                                                                aaCCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGAt     >  1:81892/1‑140 (MQ=255)                                                                                                                                aaCCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGAt     <  2:594565/140‑1 (MQ=255)                                                                                                                                aaCCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGAt     <  1:810425/140‑1 (MQ=255)                                                                                                                                aaCCCAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGAt     <  1:208331/140‑1 (MQ=255)                                                                                                                                  cccAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATgg       >  1:187311/1‑136 (MQ=255)                                                                                                                                  cccAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATgg       <  2:187311/136‑1 (MQ=255)                                                                                                                                   ccAACCTTTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGATGAg  <  2:305088/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AGCGTGCGCTACGCCTGCAGACGTTGCGATAAGCACTCCAAGTGCCAGAAGTTTGAGTTTGGTCACAATTAATCATTCCTTAAACAAATGTTTAGCGGGCGACAAAGTTTACCCGTCAATACATCGAAACCCAACC‑TTTTTACGTTTTCATTTTTTAAAGTAACTGTTTAATTTTCCTGACGCGGATGACACCGCGCTTACAGCCAAATGAATTTAGCGTTACTGGCGAGCCTGGTCTTTACATTAATTATGCAAAATTTATGGATGAG  >  NC_000913/1866407‑1866675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑