Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,949,993	(T)5→6	coding (752/1332 nt)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,949,988	1	.	T	98.0%	170.9 / ‑3.5	49	coding (747/1332 nt)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (24/24);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

ATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949857‑2950122                                                                                                                                     |                                                                                                                                        atTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑tttt‑ttgc                                                                                                                                >  2:486679/1‑137 (MQ=255)  ttGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑tttt‑ttgcg                                                                                                                               <  2:812105/140‑5 (MQ=255)   tGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑tttt‑ttgcgt                                                                                                                              >  1:130697/1‑135 (MQ=255)     gTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTgg                                                                                                                             <  1:575538/140‑1 (MQ=255)        tttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGgcgc                                                                                                                          >  1:5953/1‑140 (MQ=255)                     gggCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTACAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGc                                                                                                             <  2:565555/140‑1 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGcc                                                                                                                <  2:358321/131‑1 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGcc                                                                                                                >  1:358321/1‑131 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:43185/1‑140 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:285596/1‑140 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  1:509524/1‑140 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  2:713185/1‑140 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  1:166876/1‑140 (MQ=255)                           cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  1:90687/1‑140 (MQ=255)                                aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt                                                                                                  >  1:836621/1‑140 (MQ=255)                                aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt                                                                                                  >  2:503846/1‑140 (MQ=255)                                aTGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgt                                                                                                  <  1:526829/140‑1 (MQ=255)                                 tGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg                                                                                                 <  1:285596/140‑1 (MQ=255)                                 tGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtg                                                                                                 <  2:269614/140‑1 (MQ=255)                                      acgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCg                                                                                            <  2:497277/140‑1 (MQ=255)                                        ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          >  1:346021/1‑140 (MQ=255)                                             tatTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCAt                                                                                     <  1:713185/140‑1 (MQ=255)                                                  tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                >  2:830663/1‑140 (MQ=255)                                                    tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  1:106094/138‑1 (MQ=255)                                                    tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                >  2:106094/1‑138 (MQ=255)                                                    tcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCa                                                                              <  2:220863/140‑1 (MQ=255)                                                                         gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         <  2:509524/140‑1 (MQ=255)                                                                         gcgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcgcgc                                                         <  1:43185/140‑1 (MQ=255)                                                                          cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        <  1:503846/140‑1 (MQ=255)                                                                          cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        >  2:446480/1‑140 (MQ=255)                                                                          cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        <  1:741659/140‑1 (MQ=255)                                                                          cgcaagcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCAGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        >  2:438469/1‑140 (MQ=255)                                                                                             ccAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCa                                     <  1:830663/140‑1 (MQ=255)                                                                                              cAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAc                                    >  2:107386/1‑140 (MQ=255)                                                                                               aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAagag                                              <  1:275585/129‑1 (MQ=255)                                                                                               aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAagag                                              >  2:275585/1‑129 (MQ=255)                                                                                               aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTtct                                       >  2:257930/1‑136 (MQ=255)                                                                                               aGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTtct                                       <  1:257930/136‑1 (MQ=255)                                                                                                ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  <  2:90687/140‑1 (MQ=255)                                                                                                ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  >  1:761482/1‑140 (MQ=255)                                                                                                 gAAGATAAAGGCGATTACAACTCCGGCACGGTGCGC‑TTTTCTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCATGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgcg                                 <  2:761482/140‑1 (MQ=255)                                                                                                  aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  2:5953/140‑1 (MQ=255)                                                                                                  aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  2:156854/140‑1 (MQ=255)                                                                                                  aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  2:836621/140‑1 (MQ=255)                                                                                                     agaAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                             >  2:178181/1‑140 (MQ=255)                                                                                                     agaAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACgg                             <  2:166876/140‑1 (MQ=255)                                                                                                       aaaGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTa                           >  1:51743/1‑140 (MQ=255)                                                                                                         aGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGGAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATc                         <  2:130697/140‑1 (MQ=255)                                                                                                               ttACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACg                   <  2:346021/140‑1 (MQ=255)                                                                                                                                tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  2:46456/1‑140 (MQ=255)                                                                                                                                tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  2:454635/1‑140 (MQ=255)                                                                                                                                tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  2:537683/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        ATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949857‑2950122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑