Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,641	T→C	intergenic (‑147/+2)	satP ← / ← yaaW	acetate/succinate:H(+) symporter/putative enzyme‑specific chaperone YaaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,641	0	T	C	100.0%	131.5 / NA	37	intergenic (‑147/+2)	satP/yaaW	acetate/succinate:H(+) symporter/putative enzyme‑specific chaperone YaaW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/17);  total (20/17)

TCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCC  >  NC_000913/10514‑10768                                                                                                                                |                                                                                                                                tcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAg                                                                                                                     <  2:424638/140‑1 (MQ=255)    aatgaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAg                                                                                                                     >  1:104107/1‑137 (MQ=255)    aatgaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAg                                                                                                                     <  2:104107/137‑1 (MQ=255)        aatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGATGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCt                                                                                                              <  2:733931/140‑1 (MQ=255)            gTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAAcc                                                                                                          >  1:205479/1‑140 (MQ=255)                    atTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGgcg                                                                                                  >  2:510088/1‑140 (MQ=255)                     tttCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGgcgc                                                                                                 >  2:820772/1‑140 (MQ=255)                         ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCgcaggc                                                                                             <  2:205479/140‑1 (MQ=255)                               tAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGa                                                                                       >  2:801533/1‑140 (MQ=255)                                       gtgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                               >  1:797304/1‑140 (MQ=255)                                       gtgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                               >  1:466276/1‑140 (MQ=255)                                       gtgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                               >  2:668487/1‑140 (MQ=255)                                        tgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTa                                                                              >  2:415803/1‑140 (MQ=255)                                            ttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   >  1:61049/1‑131 (MQ=255)                                            ttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                   <  2:61049/131‑1 (MQ=255)                                                gggCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGa                                                                      <  1:510088/140‑1 (MQ=255)                                                    gcgGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCg                                                                  >  2:389803/1‑140 (MQ=255)                                                              aatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATa                                                        <  1:389803/140‑1 (MQ=255)                                                                tCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAgg                                                      <  1:195468/140‑1 (MQ=255)                                                                       gTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGcc                                               >  1:816277/1‑140 (MQ=255)                                                                        tGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCg                                              >  2:774377/1‑140 (MQ=255)                                                                                     aCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTaa                                 <  1:811651/140‑1 (MQ=255)                                                                                       gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAcc                               <  2:194123/140‑1 (MQ=255)                                                                                          cTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCa                            >  2:255141/1‑140 (MQ=255)                                                                                            tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAtt                          >  1:268355/1‑140 (MQ=255)                                                                                            tttGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCAtt                          >  2:342166/1‑140 (MQ=255)                                                                                                   ggAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGt                      >  2:475455/1‑137 (MQ=255)                                                                                                   ggAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGt                      <  1:475455/137‑1 (MQ=255)                                                                                                   ggAAAATGAAAATTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGcc                   >  1:527115/1‑140 (MQ=255)                                                                                                     aaaatgaaaatTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGc                 <  2:527115/140‑1 (MQ=255)                                                                                                     aaaatgaaaatTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGc                 >  1:859702/1‑140 (MQ=255)                                                                                                     aaaatgaaaatTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGc                 <  1:820772/140‑1 (MQ=255)                                                                                                          gaaaatTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTa            <  1:801533/140‑1 (MQ=255)                                                                                                             aatTTTCCCCGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAg         <  2:466276/140‑1 (MQ=255)                                                                                                                    cccGGTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTcc  <  1:255141/140‑1 (MQ=255)                                                                                                                        gTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAggg       >  2:467063/1‑131 (MQ=255)                                                                                                                        gTTTCCGGCATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAggg       <  1:467063/131‑1 (MQ=255)                                                                                                                                |                                                                                                                                TCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCC  >  NC_000913/10514‑10768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑