Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,084	G→C	A367P (GCC→CCC)	proY →	putative transporter ProY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,084	0	G	C	100.0%	153.0 / NA	43	A367P (GCC→CCC)	proY	putative transporter ProY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/25);  total (18/25)

TTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGTGGGGTAGCAACGACCATCGGCGGGCTGA  >  NC_000913/421948‑422213                                                                                                                                         |                                                                                                                                  tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCTGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCa                                                                                                                                <  2:782129/140‑1 (MQ=255) tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCa                                                                                                                                <  2:838926/140‑1 (MQ=255) tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCa                                                                                                                                <  1:695547/140‑1 (MQ=255) tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCa                                                                                                                                <  2:63857/140‑1 (MQ=255)   tCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACg                                                                                                                              <  2:529214/140‑1 (MQ=255)      gCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACggtg                                                                                                                           <  2:719357/140‑1 (MQ=255)      gCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACggtg                                                                                                                           <  1:581053/140‑1 (MQ=255)           aCGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACggtgtgggt                                                                                                                      <  1:793823/140‑1 (MQ=255)                                            cTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGc                                                                                               >  2:486368/1‑130 (MQ=255)                                            cTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGc                                                                                               <  1:486368/130‑1 (MQ=255)                                             tGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTc                                                                                    >  1:531576/1‑140 (MQ=255)                                                    gaCTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGcc                                                                             >  1:707330/1‑140 (MQ=255)                                                         ccGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTg                                                                        >  2:776470/1‑140 (MQ=255)                                                         ccGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTg                                                                        >  2:680911/1‑140 (MQ=255)                                                          cgcgCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTgc                                                                       >  1:495661/1‑140 (MQ=255)                                                            cgcTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTgccg                                                                     <  2:379463/140‑1 (MQ=255)                                                             gctgctGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTgccgc                                                                    <  2:640358/140‑1 (MQ=255)                                                             gctgctGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTgccgc                                                                    <  1:336241/140‑1 (MQ=255)                                                              ctgctgTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTgccg                                                                     <  1:307949/138‑1 (MQ=255)                                                              ctgctgTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTgccg                                                                     >  2:307949/1‑138 (MQ=255)                                                                 ctgTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCTTTCCGTCGCCGTTTGCCGCCaga                                                                <  1:292057/140‑1 (MQ=255)                                                                 ctgTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCaga                                                                <  2:707330/140‑1 (MQ=255)                                                                  tgTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCagaa                                                               <  2:531576/140‑1 (MQ=255)                                                                      tGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGaagaag                                                           >  1:790434/1‑140 (MQ=255)                                                                           tgtATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAg                                                      >  1:4847/1‑140 (MQ=255)                                                                                       aCATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAAttt                                          >  1:854454/1‑140 (MQ=255)                                                                                       aCATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAAttt                                          >  2:394056/1‑140 (MQ=255)                                                                                             tGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTg                                    <  1:679914/140‑1 (MQ=255)                                                                                             tGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTg                                    >  1:307730/1‑140 (MQ=255)                                                                                             tGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTg                                    >  1:403712/1‑140 (MQ=255)                                                                                             tGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTg                                    >  1:433041/1‑140 (MQ=255)                                                                                             tGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTg                                    >  2:675908/1‑140 (MQ=255)                                                                                             tGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTg                                    <  2:495661/140‑1 (MQ=255)                                                                                                  gAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCggg                               >  2:456996/1‑140 (MQ=255)                                                                                                  gAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCggg                               <  2:433041/140‑1 (MQ=255)                                                                                                  gAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCggg                               <  1:394056/140‑1 (MQ=255)                                                                                                  gAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCggg                               <  1:802093/140‑1 (MQ=255)                                                                                                   aaaaCGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGt                              >  2:441217/1‑140 (MQ=255)                                                                                                    aaaCGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGTg                             <  2:4847/140‑1 (MQ=255)                                                                                                      aCGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGAggg                           <  2:464532/140‑1 (MQ=255)                                                                                                          cTTCCTGGTGATCGCTTCGCTGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGTGGGGTAg                       >  2:100596/1‑140 (MQ=255)                                                                                                                     tcgcttcgctGGCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGTGGGGTAGCAACGACCATc            <  1:675908/140‑1 (MQ=255)                                                                                                                               ggCAACCTTCCCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGTGGGGTAGCAACGACCATCGGCGGGCTgg  <  1:100596/140‑2 (MQ=255)                                                                                                                                         |                                                                                                                                  TTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCGTTTGCCGCCAGAAGAAGTTAAGGCGCTGAAATTTAAAGTGCCGGGTGGGGTAGCAACGACCATCGGCGGGCTGA  >  NC_000913/421948‑422213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑