Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	426,178	T→C	R14R (CGT→CGC)	tgt →	tRNA‑guanine transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	426,178	0	T	C	100.0%	175.2 / NA	49	R14R (CGT→CGC)	tgt	tRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/35);  total (14/35)

ACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTT  >  NC_000913/426044‑426312                                                                                                                                       |                                                                                                                                       aCAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccg                                                                                                                                   >  2:112004/1‑140 (MQ=255) aCAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccg                                                                                                                                   >  1:384043/1‑140 (MQ=255)  cAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgc                                                                                                                                  <  2:448277/140‑1 (MQ=255)   aaTCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgcc                                                                                                                                 <  1:112004/140‑1 (MQ=255)   aaTCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgcc                                                                                                                                 <  2:143586/140‑1 (MQ=255)   aaTCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgcc                                                                                                                                 <  1:695729/140‑1 (MQ=255)   aaTCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgcc                                                                                                                                 <  1:551149/140‑1 (MQ=255)   aaTCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgcc                                                                                                                                 <  2:773084/140‑1 (MQ=255)   aaTCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgcc                                                                                                                                 <  1:353176/140‑1 (MQ=255)   aaTCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGgccgcc                                                                                                                                 <  1:217862/140‑1 (MQ=255)     tCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTg                                                                                                                               >  2:623356/1‑140 (MQ=255)     tCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTCAACTGGACACCACCGACGGTCGCGCACTCCGCTgccgcccg                                                                                                                               <  2:857870/140‑3 (MQ=255)             cAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGa                                                                                                                       <  1:399801/140‑1 (MQ=255)               aTTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATc                                                                                                                     <  1:275162/140‑1 (MQ=255)               aTTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATc                                                                                                                     >  1:237414/1‑140 (MQ=255)               aTTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATc                                                                                                                     <  1:786419/140‑1 (MQ=255)                  aaTGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAACAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTg                                                                                                                  <  2:757170/140‑1 (MQ=255)                     gAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTgg                                                                                                                              >  2:843935/1‑125 (MQ=255)                     gAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTgg                                                                                                                              <  1:843935/125‑1 (MQ=255)                      aGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGt                                                                                                              <  2:661301/140‑1 (MQ=255)                      aGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGt                                                                                                              <  2:237414/140‑1 (MQ=255)                      aGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTG‑TTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCttt                                                                                                                         <  2:630568/128‑1 (MQ=255)                      aGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTG‑TTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCttt                                                                                                                         >  1:630568/1‑128 (MQ=255)                        cgcgTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAg                                                                                                            >  2:700399/1‑140 (MQ=255)                            tCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGa                                                                                                        <  2:865466/140‑1 (MQ=255)                                  aGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGcc                                                                                                  <  2:57646/140‑1 (MQ=255)                                   gTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCt                                                                                                 <  2:296469/140‑1 (MQ=255)                                          cgcgGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTa                                                                                          <  2:371799/140‑1 (MQ=255)                                           gcggcgCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTAt                                                                                         <  2:205609/140‑1 (MQ=255)                                            cggcgCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATg                                                                                        <  1:12056/140‑1 (MQ=255)                                            cggcgCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATg                                                                                        <  1:318750/140‑1 (MQ=255)                                               cgcTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCt                                                                                     >  2:659333/1‑140 (MQ=255)                                                   ggTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTg                                                                                 <  1:623356/140‑1 (MQ=255)                                                          aaCGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTa                                                                          <  1:437125/140‑1 (MQ=255)                                                               tGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCa                                                                     <  1:835132/140‑1 (MQ=255)                                                                ggACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCAc                                                                    <  1:735984/140‑1 (MQ=255)                                                                                     ggAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGga                                               <  2:384043/140‑1 (MQ=255)                                                                                       agaAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGgaag                                             <  2:584632/140‑1 (MQ=255)                                                                                        gaAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGgaaga                                            >  1:413463/1‑140 (MQ=255)                                                                                           aaaaTGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGt                                         >  2:643499/1‑140 (MQ=255)                                                                                              aTGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGa                                      >  1:138434/1‑140 (MQ=255)                                                                                                   aTTTGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGACGGAAGAAGTTGAAGCCa                                 >  2:512323/1‑140 (MQ=255)                                                                                                      tGAACTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTg                              >  2:704550/1‑140 (MQ=255)                                                                                                          cTGGACACCACCGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGgcgc                          <  2:413463/140‑1 (MQ=255)                                                                                                                    ccGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATc                <  1:525154/140‑1 (MQ=255)                                                                                                                    ccGACGGTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATc                <  2:461684/140‑1 (MQ=255)                                                                                                                          gTCGCGCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGc          <  1:704550/140‑1 (MQ=255)                                                                                                                            cgcgCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCaa        >  2:430975/1‑140 (MQ=255)                                                                                                                            cgcgCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCaa        <  2:42319/140‑1 (MQ=255)                                                                                                                            cgcgCACGCCGCGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCaa        <  2:601964/140‑1 (MQ=255)                                                                                                                                  cgccgcGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCtt  <  1:643499/140‑1 (MQ=255)                                                                                                                                  cgccgcGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAACCCACTGGCGCGCAAATTATCCTCGGCAACATCtt  <  2:426147/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTT  >  NC_000913/426044‑426312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑