Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	474,678	G→A	P162S (CCG→TCG)	tesB ←	acyl‑CoA thioesterase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	474,678	0	G	A	100.0%	160.9 / NA	45	P162S (CCG→TCG)	tesB	acyl‑CoA thioesterase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/18);  total (27/18)

ATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTG  >  NC_000913/474550‑474810                                                                                                                                 |                                                                                                                                     aTCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCa                                                                                                                           <  2:477313/140‑1 (MQ=255)      aaGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGa                                                                                                                      >  2:823304/1‑140 (MQ=255)       aGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGAc                                                                                                                     <  1:680460/140‑1 (MQ=255)                  gCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATg                                                                                                          >  1:510923/1‑140 (MQ=255)                   cAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGa                                                                                                         >  1:818248/1‑140 (MQ=255)                        aCTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTa                                                                                                    <  2:818248/140‑1 (MQ=255)                        aCTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCACAg                                                                                                             <  2:382572/131‑1 (MQ=255)                        aCTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCACAg                                                                                                             >  1:382572/1‑131 (MQ=255)                           gatgaACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCt                                                                                                 <  2:397031/140‑1 (MQ=255)                            atgaACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCtt                                                                                                >  1:383486/1‑140 (MQ=255)                               aaCGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAAt                                                                                                       <  2:812687/130‑1 (MQ=255)                               aaCGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAAt                                                                                                       >  1:812687/1‑130 (MQ=255)                                aCGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCtt                                                                                                >  1:557405/1‑136 (MQ=255)                                aCGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCtt                                                                                                <  2:557405/136‑1 (MQ=255)                                 cgcgCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGc                                                                                           >  1:462335/1‑140 (MQ=255)                                 cgcgCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGc                                                                                           >  1:672432/1‑140 (MQ=255)                                       ggTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTggc                                                                                     <  2:510923/140‑1 (MQ=255)                                             ccGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGgcagc                                                                               <  2:383486/140‑1 (MQ=255)                                             ccGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGgcagc                                                                               <  1:279164/140‑1 (MQ=255)                                                gCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAgg                                                                            >  2:344051/1‑140 (MQ=255)                                                  aCGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTg                                                                          >  2:509435/1‑140 (MQ=255)                                                       aCCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCa                                                                     >  2:243737/1‑140 (MQ=255)                                                              gcgcGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGa                                                                 <  2:717458/137‑1 (MQ=255)                                                              gcgcGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGa                                                                 >  1:717458/1‑137 (MQ=255)                                                                         cacCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCg                                                   <  1:823304/140‑1 (MQ=255)                                                                         cacCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCg                                                   <  2:635663/140‑1 (MQ=255)                                                                         cacCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCg                                                   >  1:635663/1‑140 (MQ=255)                                                                              gACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTcc                                              <  1:243737/140‑1 (MQ=255)                                                                              gACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTcc                                              <  1:642085/140‑1 (MQ=255)                                                                                cGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGa                                            >  1:429430/1‑140 (MQ=255)                                                                                   tgtgGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAgg                                         >  2:831072/1‑140 (MQ=255)                                                                                   tgtgGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAgg                                         >  1:359791/1‑140 (MQ=255)                                                                                       gTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAgg                                     <  2:477735/140‑1 (MQ=255)                                                                                         tCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCAAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGcc                                   >  2:593576/1‑140 (MQ=255)                                                                                                 gTGACCTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGgcg                           >  1:628367/1‑140 (MQ=255)                                                                                                      cTTTCAGTGGGTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGgc                      <  1:593576/140‑1 (MQ=255)                                                                                                               ggTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGc             >  1:199044/1‑140 (MQ=255)                                                                                                               ggTTATGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGc             >  1:604621/1‑140 (MQ=255)                                                                                                                   aTGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTg         >  2:606111/1‑140 (MQ=255)                                                                                                                   aTGAAACTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGACGCTGGCGCGGACGGCATTg         >  1:365229/1‑140 (MQ=255)                                                                                                                       aaCTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGtttt     >  1:697874/1‑140 (MQ=255)                                                                                                                         cTCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGtttttt   >  2:8804/1‑140 (MQ=255)                                                                                                                          tCCACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTg  >  1:320189/1‑140 (MQ=255)                                                                                                                           ccACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCa            >  2:15043/1‑129 (MQ=255)                                                                                                                           ccACCGAACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCa            <  1:15043/129‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     ATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGCATTGTTTTTTG  >  NC_000913/474550‑474810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑