Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	549,150	T→A	I265N (ATT→AAT)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	549,150	0	T	A	100.0%	150.9 / NA	43	I265N (ATT→AAT)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/20);  total (23/20)

GATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATC  >  NC_000913/549014‑549286                                                                                                                                         |                                                                                                                                         gATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCg                                                                                                                                       <  1:77705/140‑1 (MQ=255)            cGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGc                                                                                                                            >  2:354763/1‑140 (MQ=255)                aCCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTggg                                                                                                                        >  2:386456/1‑140 (MQ=255)                    aTCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGaa                                                                                                                    <  2:267996/140‑1 (MQ=255)                    aTCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGaa                                                                                                                    <  2:298140/140‑1 (MQ=255)                    aTCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGaa                                                                                                                    <  2:543127/140‑1 (MQ=255)                      cAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACg                                                                                                                  <  2:824926/140‑1 (MQ=255)                      cAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACg                                                                                                                  <  1:374421/140‑1 (MQ=255)                           cttcCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGc                                                                                                                       <  2:562250/130‑1 (MQ=255)                           cttcCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGc                                                                                                                       >  1:562250/1‑130 (MQ=255)                                 cAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTg                                                                                                       >  2:406803/1‑140 (MQ=255)                                   aCCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTgcg                                                                                                     >  1:39067/1‑140 (MQ=255)                                       cgcgATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTg                                                                                                       >  1:565664/1‑134 (MQ=255)                                       cgcgATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTg                                                                                                       <  2:565664/134‑1 (MQ=255)                                       cgcgATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTg                                                                                                 >  2:697757/1‑140 (MQ=255)                                        gcgATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGt                                                                                                >  2:665464/1‑140 (MQ=255)                                         cgATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTa                                                                                               <  1:665464/140‑1 (MQ=255)                                              gCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGcc                                                                                                   <  2:119717/131‑1 (MQ=255)                                              gCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGcc                                                                                                   >  1:119717/1‑131 (MQ=255)                                                  aCTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCg                                                                                      >  2:674103/1‑140 (MQ=255)                                                   cTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGc                                                                                     <  2:360721/140‑1 (MQ=255)                                                    tGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGACAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACTCTCCGCa                                                                                    <  2:8928/140‑1 (MQ=255)                                                        aGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAaca                                                                                           <  1:495205/129‑1 (MQ=255)                                                        aGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAaca                                                                                           >  2:495205/1‑129 (MQ=255)                                                        aGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGt                                                                                >  2:21249/1‑140 (MQ=255)                                                                    atgCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCAcc                                                                    >  2:216343/1‑140 (MQ=255)                                                                                       cgcAGGCAGCATCGTCACGGCAATGACCCGCAGCGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAgg                                                 <  1:216343/140‑1 (MQ=255)                                                                                       cgcAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAgg                                                 <  1:119982/140‑1 (MQ=255)                                                                                       cgcAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAgg                                                 <  1:674103/140‑1 (MQ=255)                                                                                            gcagcaTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAAc                                            >  1:246780/1‑140 (MQ=255)                                                                                              agcaTCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTTAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAAccc                                          >  2:750842/1‑140 (MQ=255)                                                                                                  tCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGat                                      <  1:648722/140‑1 (MQ=255)                                                                                                          gCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGa                              >  2:581586/1‑140 (MQ=255)                                                                                                          gCAATGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGa                              >  2:226350/1‑140 (MQ=255)                                                                                                              tGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAgc                          >  1:82085/1‑140 (MQ=255)                                                                                                              tGACCCGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAgc                          >  1:495129/1‑140 (MQ=255)                                                                                                                  ccGCAACGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGa                      <  2:133102/140‑1 (MQ=255)                                                                                                                      aaCGGCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTAc                  <  2:82085/140‑1 (MQ=255)                                                                                                                          gCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGaa              >  1:858601/1‑140 (MQ=255)                                                                                                                          gCAATATGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGaa              >  1:278376/1‑140 (MQ=255)                                                                                                                              tatGTTCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCt          <  1:750842/140‑1 (MQ=255)                                                                                                                                   tCGGGAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGt     >  1:653953/1‑140 (MQ=255)                                                                                                                                      ggAATCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATc  >  2:792203/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GATTTCCTCAGCGTGACCGATCAGTTCTTCCTCAACCTCGCGATGGCTTACTGCAAGGCGGCGATGGATGCTGGCGCGATGATCCGCGCAGGCAGCATCGTCACGGCAATGACCCGCAACGGCAATATGTTCGGGATTCGGGTAAGCGGGCTGGGCGAACGCTGGTTTACTGCGCCTGTAAACACTCCGCAAGGTCTGTTTTTCACCGGCTTCTCGCAGGAGCAGGCGAACCCGGATATGGGCGATAGCGCGATTACCGAAACCTTTGGTATC  >  NC_000913/549014‑549286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑