Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	685,477	G→A	F151F (TTC→TTT)	gltK ←	glutamate/aspartate ABC transporter membrane subunit GltK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	685,477	0	G	A	100.0%	280.9 / NA	78	F151F (TTC→TTT)	gltK	glutamate/aspartate ABC transporter membrane subunit GltK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/38);  total (40/38)

CAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAGGCCGCTTCA  >  NC_000913/685344‑685609                                                                                                                                      |                                                                                                                                     cAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGcc                                                                                                                                <  1:167921/140‑1 (MQ=255)      tGCGTACCATCACGCTCACCAATGGTTGAGGCGGAACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCAGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCgg                                                                                                                           <  1:782758/140‑1 (MQ=255)        cGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCa                                                                                                                         <  1:752746/140‑1 (MQ=255)        cGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCa                                                                                                                         >  2:813235/1‑140 (MQ=255)        cGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCa                                                                                                                         <  2:199863/140‑1 (MQ=255)                 aCGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCa                                                                                                                <  2:640343/140‑1 (MQ=255)                  cGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAg                                                                                                               >  1:121536/1‑140 (MQ=255)                       aCCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCa                                                                                                          >  1:59126/1‑140 (MQ=255)                       aCCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCa                                                                                                          >  2:506868/1‑140 (MQ=255)                           aTGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGa                                                                                                      <  1:178617/140‑1 (MQ=255)                             ggTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACt                                                                                                    <  2:121536/140‑1 (MQ=255)                              gTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTg                                                                                                   <  2:59126/140‑1 (MQ=255)                                 gAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCa                                                                                                >  1:208496/1‑140 (MQ=255)                                 gAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCa                                                                                                >  1:334414/1‑140 (MQ=255)                                  aGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                                                                                               <  2:334414/140‑1 (MQ=255)                                   ggCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGa                                                                                                      <  1:371681/132‑1 (MQ=255)                                   ggCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGa                                                                                                      >  2:371681/1‑132 (MQ=255)                                    gCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTg                                                                                             <  1:541089/140‑1 (MQ=255)                                      ggTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCa                                                                                                          >  2:498821/1‑125 (MQ=255)                                      ggTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCa                                                                                                          <  1:498821/125‑1 (MQ=255)                                      ggTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAg                                                                                           <  1:288752/140‑1 (MQ=255)                                      ggTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAg                                                                                           <  1:813235/140‑1 (MQ=255)                                        tACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTc                                                                                         >  1:396453/1‑140 (MQ=255)                                        tACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTc                                                                                         >  1:728779/1‑140 (MQ=255)                                         aCGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCa                                                                                        <  1:181253/140‑1 (MQ=255)                                                           ggATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCgcgg                                                                      >  2:349069/1‑140 (MQ=255)                                                             aTAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCgcggcg                                                                    <  2:396453/140‑1 (MQ=255)                                                               aaCACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCt                                                                  <  2:335196/140‑1 (MQ=255)                                                                acacATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTc                                                                 >  2:556972/1‑140 (MQ=255)                                                                     tACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTg                                                            >  1:630521/1‑140 (MQ=255)                                                                     tACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTg                                                            >  2:274799/1‑140 (MQ=255)                                                                     tACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTg                                                            >  1:472500/1‑140 (MQ=255)                                                                      acacCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGa                                                           >  2:295611/1‑140 (MQ=255)                                                                      acacCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGa                                                           >  1:161795/1‑140 (MQ=255)                                                                       cacCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGAc                                                          >  1:461320/1‑140 (MQ=255)                                                                       cacCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGAc                                                          >  2:551506/1‑140 (MQ=255)                                                                        acCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGAcc                                                         <  1:551506/140‑1 (MQ=255)                                                                        acCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGAcc                                                         <  1:16730/140‑1 (MQ=255)                                                                          cAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCAc                                                       <  2:472500/140‑1 (MQ=255)                                                                          cAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCAc                                                       <  1:125800/140‑1 (MQ=255)                                                                            gTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGAcc                                                         >  2:614272/1‑136 (MQ=255)                                                                            gTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGAcc                                                         <  1:614272/136‑1 (MQ=255)                                                                              gAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACgaga                                                   <  2:461320/140‑1 (MQ=255)                                                                                    tCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACt                                             >  1:609447/1‑140 (MQ=255)                                                                                       tGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTg                                          >  2:524140/1‑140 (MQ=255)                                                                                         gAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGaa                                        >  2:185144/1‑140 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTg                                          >  2:862106/1‑132 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAg                                  >  1:774190/1‑140 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAg                                  >  1:745621/1‑140 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAg                                  >  1:683183/1‑140 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAg                                  >  2:809654/1‑140 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAg                                  >  1:367586/1‑140 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAg                                  <  1:185144/140‑1 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATGCCCAACGCCAGCGCGGCGCTCGACTGACCACGAGAAATACTTTg                                          <  1:862106/132‑1 (MQ=255)                                                                                               tACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCATGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAAGACTTTGAATACCAg                                  >  2:527165/1‑140 (MQ=255)                                                                                                    tGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACgg                             >  2:90773/1‑140 (MQ=255)                                                                                                    tGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACgg                             >  1:725826/1‑140 (MQ=255)                                                                                                    tGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACgg                             >  1:355370/1‑140 (MQ=255)                                                                                                    tGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGCGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACgg                             >  2:483908/1‑140 (MQ=255)                                                                                                      cccTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGAt                           <  2:355370/140‑1 (MQ=255)                                                                                                      cccTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGAt                           <  2:774190/140‑1 (MQ=255)                                                                                                      cccTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGAt                           <  1:506868/140‑1 (MQ=255)                                                                                                      cccTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCATGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGAt                           <  1:527165/140‑1 (MQ=255)                                                                                                      cccTGAGTGACCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGAt                           <  2:745621/140‑1 (MQ=255)                                                                                                       ccTGAGTGAGCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATa                          >  2:589995/1‑140 (MQ=255)                                                                                                         tgagtgagCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAAt                        >  2:842610/1‑140 (MQ=255)                                                                                                         tgagtgagCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAAt                        <  2:609447/140‑1 (MQ=255)                                                                                                         tgagtgagCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAAt                        <  2:208496/140‑1 (MQ=255)                                                                                                         tgagtgagCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAAt                        <  2:630521/140‑1 (MQ=255)                                                                                                             tgagCAGCAGCGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATctct                    <  2:327986/140‑1 (MQ=255)                                                                                                                gcagcagcGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGaa                 >  2:173223/1‑140 (MQ=255)                                                                                                                gcagcagcGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGaa                 >  2:50884/1‑140 (MQ=255)                                                                                                                       cGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAgg          <  2:367586/140‑1 (MQ=255)                                                                                                                       cGGCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAgg          <  2:161795/140‑1 (MQ=255)                                                                                                                         gCACCATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAGGcc        <  1:274799/140‑1 (MQ=255)                                                                                                                             cATCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCAAGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAGGCCGCtt    >  2:822759/1‑140 (MQ=255)                                                                                                                              aTCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAGGCCGCTTc   >  1:816238/1‑140 (MQ=255)                                                                                                                               tCGCGCGAAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAGGCCGCTTCa  <  1:589995/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     CAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCCAGCGCGGCGCTCGATTGACCACGAGAAATACTTTGAATACCAGCACGGATAATCTCTGAATAGTAGGCCGCTTCA  >  NC_000913/685344‑685609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑