Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,467	A→G	G237G (GGT→GGC)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,467	0	A	G	100.0%	180.9 / NA	50	G237G (GGT→GGC)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/29);  total (21/29)

CTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGAT  >  NC_000913/698331‑698603                                                                                                                                         |                                                                                                                                         ctTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGgcca                                                                                                                                       >  2:110115/1‑140 (MQ=255)    tCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGccacca                                                                                                                                    <  1:762571/140‑1 (MQ=255)    tCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGccacca                                                                                                                                    <  1:540586/140‑1 (MQ=255)         tCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAag                                                                                                                               <  1:704073/140‑1 (MQ=255)            ggTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAagcag                                                                                                                            >  2:650177/1‑140 (MQ=255)              tGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAgcagca                                                                                                                          <  1:154411/140‑1 (MQ=255)              tGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAgcagca                                                                                                                          <  1:757241/140‑1 (MQ=255)              tGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAgcagca                                                                                                                          <  2:732169/140‑1 (MQ=255)                  cccGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACAcc                                                                                                                      >  1:767743/1‑140 (MQ=255)                        aGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAgg                                                                                                                <  2:223889/140‑1 (MQ=255)                          aCCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGGAGCACACCGTAAGGca                                                                                                              >  2:772227/1‑140 (MQ=255)                          aCCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGca                                                                                                              <  2:286652/140‑1 (MQ=255)                            cTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGAcaca                                                                                                            <  2:305408/140‑1 (MQ=255)                                 gCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCacatcaga                                                                                                       <  2:148373/140‑1 (MQ=255)                                  cAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCacatcagac                                                                                                      <  1:795195/140‑1 (MQ=255)                                   aaaGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCacatcagaca                                                                                                     <  1:37831/140‑1 (MQ=255)                                        aGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCAcatcagacatcaga                                                                                                <  1:772227/140‑1 (MQ=255)                                            tAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGc                                                                                            >  2:264215/1‑140 (MQ=255)                                             aaCTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCt                                                                                           <  2:69037/140‑1 (MQ=255)                                                 gCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAACGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTa                                                                                       >  1:751780/1‑140 (MQ=255)                                                  cGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTaa                                                                                      >  2:114138/1‑140 (MQ=255)                                                  cGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTaa                                                                                      >  1:252468/1‑140 (MQ=255)                                                  cGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTaa                                                                                      >  1:644400/1‑140 (MQ=255)                                                    gCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACt                                                                                    >  1:326807/1‑140 (MQ=255)                                                    gCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACt                                                                                    >  2:676721/1‑140 (MQ=255)                                                       accaGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGaa                                                                                 <  1:114138/140‑1 (MQ=255)                                                              ttCAGAGCGTTCCTGATCTTCCACGCGGCGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAGGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCa                                                                          <  2:77378/140‑1 (MQ=255)                                                                      gTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGa                                                                  >  2:185121/1‑140 (MQ=255)                                                                             aTCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCt                                                           >  1:698276/1‑140 (MQ=255)                                                                                  ccACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGttt                                                      >  1:257897/1‑140 (MQ=255)                                                                                   cACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGtttt                                                     <  1:277914/140‑1 (MQ=255)                                                                                      gcgACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGt                                                  <  2:257897/140‑1 (MQ=255)                                                                                         aCGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCgg                                               >  2:55673/1‑140 (MQ=255)                                                                                          cGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGt                                              >  2:102015/1‑140 (MQ=255)                                                                                              cTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACg                                          <  1:650177/140‑1 (MQ=255)                                                                                              cTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACg                                          <  2:751780/140‑1 (MQ=255)                                                                                                gCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGtt                                        <  2:644400/140‑1 (MQ=255)                                                                                                   tATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATc                                     <  2:326807/140‑1 (MQ=255)                                                                                                      ttCTTGGTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCttt                                  <  1:647746/140‑1 (MQ=255)                                                                                                           ggTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAc                               <  1:592981/138‑1 (MQ=255)                                                                                                           ggTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAc                               >  2:592981/1‑138 (MQ=255)                                                                                                           ggTGATAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCg                             >  2:24095/1‑140 (MQ=255)                                                                                                               aTAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATc                         <  2:252468/140‑1 (MQ=255)                                                                                                               aTAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATc                         <  2:698276/140‑1 (MQ=255)                                                                                                                tAGCGGAAATAATTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCg                        >  2:236410/1‑140 (MQ=255)                                                                                                                           atTGAGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCa             >  2:854512/1‑140 (MQ=255)                                                                                                                               aGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcg         <  1:676721/140‑1 (MQ=255)                                                                                                                               aGGAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcg         >  1:604002/1‑140 (MQ=255)                                                                                                                                 gAATCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgcg       <  1:24095/140‑1 (MQ=255)                                                                                                                                    tCCAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatg    >  1:392035/1‑140 (MQ=255)                                                                                                                                      cAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatgat  <  2:604002/140‑1 (MQ=255)                                                                                                                                      cAGGCCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatgat  <  2:292528/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTTTCAGATCCGGTGAACCCGGCAGACCTACAGCAAAGGAGTGTAACTGCGGCCACCAGGCTTCAGAGCGTTCCTGATCTTCCACGCGACGGGCTGCGTATTTCTTGGTGATAGCGGAAATAATTGAGGAATCCAGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGAT  >  NC_000913/698331‑698603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑