Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	169,672	G→A	G730D (GGC→GAC)	fhuA →	ferrichrome outer membrane transporter/phage receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	169,672	0	G	A	100.0%	161.3 / NA	46	G730D (GGC→GAC)	fhuA	ferrichrome outer membrane transporter/phage receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/23);  total (23/23)

TGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCA  >  NC_000913/169537‑169808                                                                                                                                        |                                                                                                                                         tGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTAGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGactgc                                                                                                                                      >  1:257484/1‑140 (MQ=255)   ggAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCtt                                                                                                                                    >  1:679451/1‑140 (MQ=255)               gTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGa                                                                                                                        <  1:558644/140‑1 (MQ=255)                tCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGaa                                                                                                                       >  1:96864/1‑140 (MQ=255)                  gTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACg                                                                                                                     >  2:650767/1‑140 (MQ=255)                  gTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACg                                                                                                                     <  1:411711/140‑1 (MQ=255)                  gTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGGGGCGCTGCATGTTAACAACCTGGTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGTTGCGCAGGACg                                                                                                                     >  2:567944/1‑140 (MQ=255)                        gCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGt                                                                                                               >  2:841355/1‑140 (MQ=255)                             aGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTg                                                                                                          <  2:96864/140‑1 (MQ=255)                                aCGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaa                                                                                                       >  2:851912/1‑140 (MQ=255)                                    tATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaaccgc                                                                                                   >  2:212218/1‑140 (MQ=255)                                      tGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaaccgcaa                                                                                                 >  1:136461/1‑140 (MQ=255)                                             gcgcGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaaccg                                                                                                    <  1:299759/130‑1 (MQ=255)                                             gcgcGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTgcaaccg                                                                                                    >  2:299759/1‑130 (MQ=255)                                              cgcgAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGt                                                                                         >  2:545646/1‑140 (MQ=255)                                              cgcgAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGt                                                                                         >  1:409124/1‑140 (MQ=255)                                                cgAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGttt                                                                                       <  2:679451/140‑1 (MQ=255)                                                  aGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCt                                                                                     >  2:344781/1‑140 (MQ=255)                                                  aGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCt                                                                                     <  1:841355/140‑1 (MQ=255)                                                                  tCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTgggg                                                                     >  1:242489/1‑140 (MQ=255)                                                                  tCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTgggg                                                                     <  2:409124/140‑1 (MQ=255)                                                                   ccAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGc                                                                    <  1:212218/140‑1 (MQ=255)                                                                        gTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGa                                                               <  1:121994/140‑1 (MQ=255)                                                                            cgcTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTc                                                           >  2:661296/1‑140 (MQ=255)                                                                                gCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTg                                                       <  1:650767/140‑1 (MQ=255)                                                                                  aTGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGcc                                                     <  1:661296/140‑1 (MQ=255)                                                                                    gTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCa                                                   <  1:851912/140‑1 (MQ=255)                                                                                         caacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTca                                              <  2:136461/140‑1 (MQ=255)                                                                                          aacCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcac                                             <  2:786953/140‑1 (MQ=255)                                                                                           acCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTcaca                                            >  1:779721/1‑140 (MQ=255)                                                                                            ccTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACaa                                           >  1:595320/1‑140 (MQ=255)                                                                                               gTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGtt                                        >  1:597358/1‑140 (MQ=255)                                                                                                    aTCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGc                                     >  1:154796/1‑138 (MQ=255)                                                                                                    aTCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGc                                     <  2:154796/138‑1 (MQ=255)                                                                                                    aTCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTg                                   <  2:257484/140‑1 (MQ=255)                                                                                                    aTCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTg                                   <  2:242489/140‑1 (MQ=255)                                                                                                    aTCGTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTg                                   <  2:779721/140‑1 (MQ=255)                                                                                                       gTGAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGATCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTa                                <  2:110555/140‑1 (MQ=255)                                                                                                         gAATACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATg                              <  2:595320/140‑1 (MQ=255)                                                                                                            tACGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTT‑CAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAgg                          >  1:487187/1‑140 (MQ=255)                                                                                                              cGTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGa                         <  1:545646/140‑1 (MQ=255)                                                                                                               gTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGaa                        >  2:507951/1‑140 (MQ=255)                                                                                                               gTCGCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGaa                        >  2:797872/1‑140 (MQ=255)                                                                                                                  gCCAGCTGCTTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATac                     <  2:351541/140‑1 (MQ=255)                                                                                                                       ctgctTTAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGaa                >  2:514864/1‑140 (MQ=255)                                                                                                                           tttAACACTTATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCAt            <  1:344781/140‑1 (MQ=255)                                                                                                                                    tATGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATAcc   >  2:372740/1‑140 (MQ=255)                                                                                                                                     aTGACTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCa  >  1:566548/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TGGGAAGTTATACGGTCGTGGATGCGTTAGTACGTTATGATCTGGCGCGAGTCGGCATGGCTGGCTCCAACGTGGCGCTGCATGTTAACAACCTGTTCGATCGTGAATACGTCGCCAGCTGCTTTAACACTTATGGCTGCTTCTGGGGCGCAGAACGTCAGGTCGTTGCAACCGCAACCTTCCGTTTCTAATTTCTCTTTTGGGGCACGGATTTCCGTGCCCATTTCACAAGTTGGCTGTTATGCAGGAATACACGAATCATTCCGATACCA  >  NC_000913/169537‑169808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑