Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,296,446	T→C	Y559C (TAC→TGC)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,296,446	0	T	C	100.0%	171.9 / NA	48	Y559C (TAC→TGC)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

TTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGG  >  NC_000913/1296310‑1296565                                                                                                                                         |                                                                                                                        ttCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGcaca                                                                                                                      <  1:622795/140‑1 (MQ=255) ttCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGcaca                                                                                                                      <  2:280496/140‑1 (MQ=255)  tCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACAt                                                                                                                     <  2:774899/140‑1 (MQ=255)    aGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATaa                                                                                                                   <  1:243136/140‑1 (MQ=255)       aCCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAAccc                                                                                                                >  1:234479/1‑140 (MQ=255)           gTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACAt                                                                                                            >  2:399490/1‑140 (MQ=255)             aCCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGa                                                                                                          >  2:167958/1‑140 (MQ=255)                     tggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTcgcg                                                                                                  <  1:350986/140‑1 (MQ=255)                      ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcgg                                                                                                 >  1:108020/1‑140 (MQ=255)                      ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcgg                                                                                                 >  2:838091/1‑140 (MQ=255)                        tggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcggcg                                                                                               <  2:108020/140‑1 (MQ=255)                        tggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcggcg                                                                                               <  2:234479/140‑1 (MQ=255)                         ggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGt                                                                                              >  1:190317/1‑140 (MQ=255)                            gACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCa                                                                                           >  2:757789/1‑140 (MQ=255)                             aCAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTcgcg                                                                                                  <  2:263007/132‑1 (MQ=255)                             aCAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTcgcg                                                                                                  >  1:263007/1‑132 (MQ=255)                             aCAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCAt                                                                                          <  1:838091/140‑1 (MQ=255)                                 cGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCggg                                                                                      >  2:803797/1‑140 (MQ=255)                                  gATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCgggg                                                                                     <  1:399490/140‑1 (MQ=255)                                                tCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAgc                                                                       >  1:664272/1‑140 (MQ=255)                                                 cACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCgggg                                                                                     >  2:713842/1‑125 (MQ=255)                                                 cACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCgggg                                                                                     <  1:713842/125‑1 (MQ=255)                                                              gggAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTc                                                         <  2:664272/140‑1 (MQ=255)                                                                      gTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGa                                                 <  1:757789/140‑1 (MQ=255)                                                                        aGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAg                                               <  1:803797/140‑1 (MQ=255)                                                                           tACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAg                                            <  2:190317/140‑1 (MQ=255)                                                                           tACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAg                                            <  1:391411/140‑1 (MQ=255)                                                                                ttACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGc                                       >  2:316066/1‑140 (MQ=255)                                                                                        atCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTg                               >  1:721237/1‑140 (MQ=255)                                                                                        atCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTg                               >  1:541301/1‑140 (MQ=255)                                                                                        atCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTg                               <  1:167958/140‑1 (MQ=255)                                                                                         tCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGc                              >  1:235300/1‑140 (MQ=255)                                                                                          ccATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCa                             <  1:316066/140‑1 (MQ=255)                                                                                             tAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCt                          <  1:755374/140‑1 (MQ=255)                                                                                               aaGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCttt                        >  2:497834/1‑140 (MQ=255)                                                                                                agcgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTa                       >  1:762750/1‑140 (MQ=255)                                                                                                  cgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacg                     >  1:119143/1‑140 (MQ=255)                                                                                                  cgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacg                     >  2:57776/1‑140 (MQ=255)                                                                                                  cgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacg                     >  1:482371/1‑140 (MQ=255)                                                                                                    cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   >  2:41862/1‑140 (MQ=255)                                                                                                    cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   <  2:721237/140‑1 (MQ=255)                                                                                                    cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   <  2:762750/140‑1 (MQ=255)                                                                                                    cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   >  2:774688/1‑140 (MQ=255)                                                                                                    cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   >  2:838492/1‑140 (MQ=255)                                                                                                     agcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaac                  >  1:522469/1‑140 (MQ=255)                                                                                                      gcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaacg                 <  2:746033/140‑1 (MQ=255)                                                                                                           aGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCt            <  2:482371/140‑1 (MQ=255)                                                                                                                ttCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAggg       <  1:57776/140‑1 (MQ=255)                                                                                                                  cGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAgggtc     <  2:235300/140‑1 (MQ=255)                                                                                                                     aGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAgggtcggg  <  1:632174/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                        TTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGG  >  NC_000913/1296310‑1296565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑