Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,705	A→G	T288A (ACC→GCC)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,705	0	A	G	100.0%	134.9 / NA	38	T288A (ACC→GCC)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

GTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCG  >  NC_000913/1738570‑1738834                                                                                                                                        |                                                                                                                                  gTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCAc                                                                                                                               >  2:770602/1‑140 (MQ=255)            tggCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGc                                                                                                                    >  2:128290/1‑140 (MQ=255)                       tGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCg                                                                                                         <  1:6934/140‑1 (MQ=255)                        ggCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGc                                                                                                        >  1:159194/1‑140 (MQ=255)                              gggCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTg                                                                                                  >  2:609606/1‑140 (MQ=255)                                       ttGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGt                                                                                         <  2:159194/140‑1 (MQ=255)                                          tgctgcTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCa                                                                                      <  2:828719/140‑1 (MQ=255)                                             tgctgATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACa                                                                                   <  1:770602/140‑1 (MQ=255)                                             tgctgATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACa                                                                                   <  2:460890/140‑1 (MQ=255)                                             tgctgATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACa                                                                                   <  1:800270/140‑1 (MQ=255)                                               ctgATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATg                                                                                 >  2:182284/1‑140 (MQ=255)                                                  atgaAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACa                                                                                   >  1:312655/1‑135 (MQ=255)                                                  atgaAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACa                                                                                   <  2:312655/135‑1 (MQ=255)                                                            ccTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACa                                                                                   <  2:502128/125‑1 (MQ=255)                                                            ccTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACa                                                                                   >  1:502128/1‑125 (MQ=255)                                                               gcgcGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGatcgtatcg                                                                 >  1:185519/1‑140 (MQ=255)                                                                    tCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTcaac                                                            >  1:531309/1‑140 (MQ=255)                                                                      ccGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTAGATCGTATCGTcaacaa                                                          <  1:182284/140‑1 (MQ=255)                                                                               gTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTgaaga                                                 >  1:565321/1‑140 (MQ=255)                                                                                tttCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTgaagaa                                                <  1:609606/140‑1 (MQ=255)                                                                                   cGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCg                                             <  1:592147/140‑1 (MQ=255)                                                                                   cGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCg                                             <  2:257851/140‑1 (MQ=255)                                                                                     cTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCa                                           <  1:792015/140‑1 (MQ=255)                                                                                     cTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGCCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCa                                           <  2:475652/140‑1 (MQ=255)                                                                                      tGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAg                                          >  2:797131/1‑140 (MQ=255)                                                                                       gATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGt                                         <  2:369297/140‑1 (MQ=255)                                                                                        aTCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTc                                        >  2:422492/1‑140 (MQ=255)                                                                                          cGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCg                                             >  2:624826/1‑133 (MQ=255)                                                                                          cGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCg                                             <  1:624826/133‑1 (MQ=255)                                                                                             ttATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTg                                   <  1:128290/140‑1 (MQ=255)                                                                                             ttATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTg                                   <  1:797131/140‑1 (MQ=255)                                                                                                     aCGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCAt                           >  2:315892/1‑140 (MQ=255)                                                                                                       gTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATcc                         <  2:613872/140‑1 (MQ=255)                                                                                                       gTGCGCAACGCGCGCTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATcc                         >  1:613872/1‑140 (MQ=255)                                                                                                                 cgcgcTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCttgatt               <  2:531309/140‑1 (MQ=255)                                                                                                                   cgcTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCttgattga             <  2:565321/140‑1 (MQ=255)                                                                                                                    gcTATTTTACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGaa            >  2:422108/1‑140 (MQ=255)                                                                                                                           tACGCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCt     <  2:185519/140‑1 (MQ=255)                                                                                                                              gCCGGCGCTGGCCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCg  >  2:307197/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  GTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACGTGCGCAACGCGCGCTATTTTACGCCGGCGCTGACCACGATCCATCAGCCAAAAGATTCGCTGGGTGAAACAGCGTTCAACATGCTGTTGGATCGTATCGTCAACAAACGTGAAGAACCGCAGTCTATTGAAGTGCATCCGCGCTTGATTGAACGCCGCTCCG  >  NC_000913/1738570‑1738834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑