Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	188,389	C→T	A88T (GCA→ACA)	glnD ←	PII uridylyltransferase/uridylyl removing enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	188,389	0	C	T	100.0%	167.5 / NA	48	A88T (GCA→ACA)	glnD	PII uridylyltransferase/uridylyl removing enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/23);  total (25/23)

CAGCGTTAACAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGCCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACG  >  NC_000913/188261‑188518                                                                                                                                 |                                                                                                                                  cagcGTTAACAGCTCGCCCACTTTTTGCCCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCaa                                                                                                                        >  1:388435/1‑140 (MQ=255)    cGTTAACAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATc                                                                                                                      >  2:490511/1‑139 (MQ=255)    cGTTAACAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATc                                                                                                                      <  1:490511/139‑1 (MQ=255)    cGTTAACAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCt                                                                                                                     >  1:755937/1‑140 (MQ=255)    cGTTAACAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCt                                                                                                                     >  1:249595/1‑140 (MQ=255)      ttAACAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTgg                                                                                                                   >  1:318973/1‑140 (MQ=255)         aCAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTg                                                                                                                >  1:238954/1‑140 (MQ=255)         aCAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTg                                                                                                                >  2:452435/1‑140 (MQ=255)             cTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATc                                                                                                            <  2:5517/140‑1 (MQ=255)              tCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATcc                                                                                                           <  2:750792/140‑1 (MQ=255)                gCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCg                                                                                                         >  1:366846/1‑140 (MQ=255)                gCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCg                                                                                                         >  1:703437/1‑140 (MQ=255)                 cccACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGc                                                                                                        >  2:705893/1‑140 (MQ=255)                   cACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCtt                                                                                                      <  2:703437/140‑1 (MQ=255)                   cACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCtt                                                                                                      <  2:318973/140‑1 (MQ=255)                   cACTTTTTGCCCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCtt                                                                                                      <  2:388435/140‑1 (MQ=255)                                tGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCg                                                                                         >  2:41719/1‑140 (MQ=255)                                tGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCg                                                                                         >  1:156827/1‑140 (MQ=255)                                tGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGAGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCg                                                                                         >  1:393073/1‑140 (MQ=255)                                  aTCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCAt                                                                                              >  1:543185/1‑133 (MQ=255)                                  aTCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCAt                                                                                              <  2:543185/133‑1 (MQ=255)                                         gggAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAg                                                                                <  1:685213/140‑1 (MQ=255)                                            aGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTg                                                                             <  2:156827/140‑1 (MQ=255)                                                       gCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGTCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGTTAAACt                                                                  <  2:399950/140‑1 (MQ=255)                                                        cTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACta                                                                 >  2:677352/1‑139 (MQ=255)                                                              aTCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTgcg                                                           <  2:238954/140‑1 (MQ=255)                                                                cAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTgcgcg                                                         <  2:393073/140‑1 (MQ=255)                                                                  gTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGcc                                                       >  2:823862/1‑140 (MQ=255)                                                                  gTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGcc                                                       >  2:188042/1‑140 (MQ=255)                                                                  gTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGcc                                                       >  1:744190/1‑140 (MQ=255)                                                                             tCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTg                                            >  2:440491/1‑140 (MQ=255)                                                                                  aaGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTg                                       <  1:452435/140‑1 (MQ=255)                                                                                    gTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCa                                     <  1:823862/140‑1 (MQ=255)                                                                                    gTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCa                                     <  1:440491/140‑1 (MQ=255)                                                                                       gATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCagag                                  >  2:642048/1‑140 (MQ=255)                                                                                        aTGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAgaga                                 <  2:744190/140‑1 (MQ=255)                                                                                             gCTCGCCACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCa                            <  1:642048/140‑1 (MQ=255)                                                                                                   cACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCa                      <  2:403942/140‑1 (MQ=255)                                                                                                   cACGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCa                      >  2:581275/1‑140 (MQ=255)                                                                                                    aCGACCGTAGCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCaa                     <  1:705893/140‑1 (MQ=255)                                                                                                             gCCACCGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCAc            <  2:304728/140‑1 (MQ=255)                                                                                                                  cGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGc                 >  2:433896/1‑130 (MQ=255)                                                                                                                  cGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGc                 <  1:433896/130‑1 (MQ=255)                                                                                                                  cGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGc       <  1:581275/140‑1 (MQ=255)                                                                                                                  cGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGc       >  2:608533/1‑140 (MQ=255)                                                                                                                  cGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGc       <  1:41719/140‑1 (MQ=255)                                                                                                                  cGACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGc       >  2:145600/1‑140 (MQ=255)                                                                                                                   gACGGCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGcc      >  1:738660/1‑140 (MQ=255)                                                                                                                       gCGACCAATGTCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACg  <  1:57661/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  CAGCGTTAACAGCTCGCCCACTTTTTGCGCCTGATCGTCCGGGAGCTTTTTACGGCTTAAAATCAGTAAATCGACGTCTGAAAGTGGATGCAGCTCGCCACGACCGTAGCCACCGACGGCGACCAATGCCAGGTCGGCAATCTGGCTGAATCCCGCTTCAATCCATAATCGTTGCAGGAGCTGGTCGATAAACTCGGTGCGCGCCTCAATCAACTGTTCTGCAGAGATCCCATTGTCAAAGGCATCACCCAGCCAACG  >  NC_000913/188261‑188518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑