Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,935,978	T→C	T113A (ACC→GCC)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,935,978	0	T	C	100.0%	147.0 / NA	42	T113A (ACC→GCC)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/19);  total (23/19)

TCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAA  >  NC_000913/1935842‑1936110                                                                                                                                         |                                                                                                                                     tCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGAt                                                                                                                                   >  2:638266/1‑140 (MQ=255)    tGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACg                                                                                                                                >  2:595708/1‑140 (MQ=255)       gAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGAt                                                                                                                              <  2:832668/139‑1 (MQ=255)       gAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGAt                                                                                                                              >  1:832668/1‑139 (MQ=255)             gCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTg                                                                                                                       >  1:787402/1‑140 (MQ=255)                  cGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCa                                                                                                                  >  1:855409/1‑140 (MQ=255)                         cccAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATcgc                                                                                                           >  2:786237/1‑140 (MQ=255)                              gggTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCga                                                                                                      <  1:633689/139‑1 (MQ=255)                                gTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCgaga                                                                                                    <  2:855409/140‑1 (MQ=255)                                gTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCgaga                                                                                                    <  2:376514/140‑1 (MQ=255)                                        aTGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGaa                                                                                            >  1:247319/1‑140 (MQ=255)                                        aTGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGaa                                                                                            <  2:121296/140‑1 (MQ=255)                                             tACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATgcag                                                                                       >  1:6917/1‑140 (MQ=255)                                               cgcgTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGTTCCAGCATCGCGCCGAGACGGCTGAATgcagca                                                                                     >  1:12490/1‑140 (MQ=255)                                               cgcgTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATgcagca                                                                                     <  1:251404/140‑1 (MQ=255)                                               cgcgTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATgcagca                                                                                     >  2:251404/1‑140 (MQ=255)                                               cgcgTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATgcagca                                                                                     >  2:33035/1‑140 (MQ=255)                                                     ccGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCa                                                                               >  2:116438/1‑140 (MQ=255)                                                       ggTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCAtt                                                                             <  2:247319/140‑1 (MQ=255)                                                             gCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATc                                                                       <  2:6917/140‑1 (MQ=255)                                                                   aGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGAtt                                                                 >  1:251921/1‑140 (MQ=255)                                                                    gTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTa                                                                >  2:239602/1‑140 (MQ=255)                                                                             cGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGAt                                                                  <  1:184751/129‑1 (MQ=255)                                                                             cGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGAt                                                                  >  2:184751/1‑129 (MQ=255)                                                                             cGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAAccc                                                       >  2:812673/1‑140 (MQ=255)                                                                                  aGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACg                                                  <  2:794010/140‑1 (MQ=255)                                                                                        ttGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACg                                                  >  1:375220/1‑134 (MQ=255)                                                                                        ttGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACg                                                  <  2:375220/134‑1 (MQ=255)                                                                                         tGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCa                                                      <  1:9443/129‑1 (MQ=255)                                                                                         tGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCa                                                      >  2:9443/1‑129 (MQ=255)                                                                                         tGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTc                                           >  2:349416/1‑140 (MQ=255)                                                                                         tGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTc                                           >  2:479016/1‑140 (MQ=255)                                                                                         tGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTc                                           >  1:777610/1‑140 (MQ=255)                                                                                         tGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTc                                           >  1:630617/1‑140 (MQ=255)                                                                                          gCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCa                                          <  1:116438/140‑1 (MQ=255)                                                                                               ttGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGgtg                                     <  1:162496/140‑1 (MQ=255)                                                                                               ttGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGgtg                                     <  1:786237/140‑1 (MQ=255)                                                                                                     cAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGTTCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCAt                               <  2:12490/140‑1 (MQ=255)                                                                                                     cAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCAt                               <  1:638266/140‑1 (MQ=255)                                                                                                                      aTGGCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGt              <  2:777610/140‑1 (MQ=255)                                                                                                                        ggCAAAGTAGTTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGttt            >  1:644445/1‑140 (MQ=255)                                                                                                                                agtTAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACTTTCATCAATGGTTTCTTTCATg    <  1:595708/140‑1 (MQ=255)                                                                                                                                  ttAATGGCGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGaa  >  2:480452/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATCGAGATTACAAAAATCCAGACGTGCACTCAGGGTGTCCCATAAACCTTCATCAATGGTTTCTTTCATGAA  >  NC_000913/1935842‑1936110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑