Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,122,619	T→C	T121A (ACC→GCC)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,122,619	0	T	C	100.0%	204.8 / NA	57	T121A (ACC→GCC)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

CCGAACAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGTATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCG  >  NC_000913/2122498‑2122755                                                                                                                          |                                                                                                                                         ccGAACAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCg                                                                                                                        <  1:806607/140‑1 (MQ=255)    aaCAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATCGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGGCGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGc                                                                                                                     <  2:799111/140‑1 (MQ=255)    aaCAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGc                                                                                                                     >  1:392520/1‑140 (MQ=255)     aCAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGcc                                                                                                                    >  1:15922/1‑140 (MQ=255)      cAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGccc                                                                                                                   >  2:98299/1‑140 (MQ=255)      cAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCAACCGCGTTTGGTTTCATCGACGGTAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGccc                                                                                                                   >  2:861212/1‑140 (MQ=255)         gTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCt                                                                                                                >  1:597370/1‑140 (MQ=255)                aCGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAAc                                                                                                         <  1:168144/140‑1 (MQ=255)                aCGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAAc                                                                                                         <  1:420395/140‑1 (MQ=255)                aCGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCACCTCGCGAAc                                                                                                         >  2:358668/1‑140 (MQ=255)                 cGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAg                                                                                                                      <  2:489113/126‑1 (MQ=255)                 cGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAg                                                                                                                      >  1:489113/1‑126 (MQ=255)                      gCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCtt                                                                                                   <  1:741804/140‑1 (MQ=255)                       cGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTc                                                                                                  <  1:366819/140‑1 (MQ=255)                       cGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTc                                                                                                  >  1:804825/1‑140 (MQ=255)                       cGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTc                                                                                                  >  1:686108/1‑140 (MQ=255)                         tCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCAt                                                                                                <  1:829688/140‑1 (MQ=255)                          cACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATg                                                                                               <  2:241869/140‑1 (MQ=255)                           aCGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTcg                                                                                                              <  2:195069/124‑1 (MQ=255)                           aCGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTcg                                                                                                              >  1:195069/1‑124 (MQ=255)                           aCGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGc                                                                                              >  1:620719/1‑140 (MQ=255)                           aCGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGc                                                                                              >  1:681756/1‑140 (MQ=255)                           aCGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGc                                                                                              >  1:791452/1‑140 (MQ=255)                                        tGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCAt                                                                                 <  1:420012/140‑1 (MQ=255)                                        tGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCAt                                                                                 <  2:392520/140‑1 (MQ=255)                                        tGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCAt                                                                                 <  1:60473/140‑1 (MQ=255)                                               aGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTa                                                                          >  2:465591/1‑140 (MQ=255)                                               aGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTa                                                                          >  1:265359/1‑140 (MQ=255)                                               aGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTa                                                                          >  2:775706/1‑140 (MQ=255)                                                  gACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCACCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtgg                                                                       <  1:358668/140‑1 (MQ=255)                                                   aCCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtggc                                                                      >  2:775933/1‑140 (MQ=255)                                                   aCCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtggc                                                                      >  2:281237/1‑140 (MQ=255)                                                   aCCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtggc                                                                      >  1:672533/1‑140 (MQ=255)                                                    ccGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtggct                                                                     >  2:404689/1‑140 (MQ=255)                                                    ccGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtggct                                                                     >  2:267271/1‑140 (MQ=255)                                                     cgcgTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtggctg                                                                    <  2:571768/140‑1 (MQ=255)                                                     cgcgTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTAtggctg                                                                    <  2:597370/140‑1 (MQ=255)                                                        gTTTGGTTTCATCGACGGTAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCg                                                                 <  1:861212/140‑1 (MQ=255)                                                          ttGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGt                                                               <  1:404689/140‑1 (MQ=255)                                                                  aTCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCaa                                                       >  1:815601/1‑140 (MQ=255)                                                                         ggaggaAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATgccgcc                                                <  1:775706/140‑1 (MQ=255)                                                                                 gTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGc                                        <  2:189237/140‑1 (MQ=255)                                                                                  tCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGcc                                       <  1:465591/140‑1 (MQ=255)                                                                                    gTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCga                                     >  2:183850/1‑140 (MQ=255)                                                                                          ttCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGc                                        >  2:452415/1‑131 (MQ=255)                                                                                          ttCAGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGc                                        <  1:452415/131‑1 (MQ=255)                                                                                             aGCCAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACg                            >  2:144251/1‑140 (MQ=255)                                                                                                cAGACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTgg                         <  1:267271/140‑1 (MQ=255)                                                                                                  gACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCg                       >  1:234433/1‑140 (MQ=255)                                                                                                  gACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCg                       <  2:804825/140‑1 (MQ=255)                                                                                                  gACGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCg                       <  2:686108/140‑1 (MQ=255)                                                                                                   aCGCTGGACGTCGCGCAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGa                      >  1:490964/1‑140 (MQ=255)                                                                                                               gcgcAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCtt          <  1:775933/140‑1 (MQ=255)                                                                                                               gcgcAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCtt          <  2:791452/140‑1 (MQ=255)                                                                                                               gcgcAGTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCtt          <  1:98299/140‑1 (MQ=255)                                                                                                                    gTCCGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTg     <  1:281237/140‑1 (MQ=255)                                                                                                                       cGGCATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCg  <  2:490964/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                         CCGAACAGGTGATCAACGTAAGCGTCACGCAGGTTGATTTGCTGATAGCGACCGCGTTTGGTTTCATCGACGGGAGGAAAGTCGTTGGCTTCAGCCAGACGCTGGACGTCGCGCAGTCCGGTATCTCCGCTGATCGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCG  >  NC_000913/2122498‑2122755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑