Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,134,271 T→C E461G (GAA→GGA)  wzc ← protein‑tyrosine kinase Wzc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,134,2710TC100.0% 138.4 / NA 39E461G (GAA→GGA) wzcprotein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/21);  total (18/21)

CCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCT  >  NC_000913/2134137‑2134406
                                                                                                                                      |                                                                                                                                       
ccAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTcc                                                                                                                                    >  2:777912/1‑140 (MQ=255)
ccAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTcc                                                                                                                                    >  1:754873/1‑140 (MQ=255)
      cGGTTGGATCCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACt                                                                                                                              <  2:498606/140‑1 (MQ=255)
         ttGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGc                                                                                                                           >  2:8750/1‑140 (MQ=255)
          tGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCg                                                                                                                          <  1:8750/140‑1 (MQ=255)
           ggATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCgg                                                                                                                         <  1:517141/140‑1 (MQ=255)
             aTTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGc                                                                                                                       <  2:562661/140‑1 (MQ=255)
              ttCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCt                                                                                                                      >  1:384402/1‑140 (MQ=255)
                ccccACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCgg                                                                                                                         <  2:253711/135‑1 (MQ=255)
                ccccACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCgg                                                                                                                         >  1:253711/1‑135 (MQ=255)
                   cACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCaa                                                                                                                 >  2:627229/1‑140 (MQ=255)
                        ccAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGc                                                                                                                       <  2:681177/129‑1 (MQ=255)
                        ccAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGc                                                                                                                       >  1:681177/1‑129 (MQ=255)
                          aGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCcgcg                                                                                                          <  1:611409/140‑1 (MQ=255)
                          aGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCcgcg                                                                                                          <  1:191564/140‑1 (MQ=255)
                           gTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGa                                                                                                         >  1:724973/1‑140 (MQ=255)
                                      tGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGa                                                                                              >  1:596900/1‑140 (MQ=255)
                                      tGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGa                                                                                              >  1:400702/1‑140 (MQ=255)
                                        tttATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAgc                                                                                            >  1:662905/1‑140 (MQ=255)
                                                  ttAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGcac                                                                                  >  1:90059/1‑140 (MQ=255)
                                                     aTCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACAcc                                                                               <  2:724973/140‑1 (MQ=255)
                                                     aTCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACAcc                                                                               <  2:366/140‑1 (MQ=255)
                                                               tGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGaa                                                                     <  2:662905/140‑1 (MQ=255)
                                                                 gTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAg                                                                   >  1:416602/1‑140 (MQ=255)
                                                                   tttGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCa                                                                 >  1:507487/1‑140 (MQ=255)
                                                                    ttGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCAt                                                                <  1:627229/140‑1 (MQ=255)
                                                                    ttGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCAt                                                                <  1:82760/140‑1 (MQ=255)
                                                                     tGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATc                                                               <  2:90059/140‑1 (MQ=255)
                                                                          cTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGcc                                                          <  1:174546/140‑1 (MQ=255)
                                                                                   gcTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAAt                                                 <  1:777912/140‑1 (MQ=255)
                                                                                       tCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATcgcg                                             >  1:139010/1‑140 (MQ=255)
                                                                                             aTTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAgg                                       <  1:419242/140‑1 (MQ=255)
                                                                                                         ggATGCTGGCATAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCt                           >  2:517650/1‑140 (MQ=255)
                                                                                                                    tAGACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGttt                >  2:554173/1‑140 (MQ=255)
                                                                                                                      gACGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCa              <  1:39583/140‑1 (MQ=255)
                                                                                                                       aCGCTAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAg             <  1:554173/140‑1 (MQ=255)
                                                                                                                           tAATACCGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACg         >  2:799474/1‑140 (MQ=255)
                                                                                                                                 cGTGTCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGc   <  2:507487/140‑1 (MQ=255)
                                                                                                                                  gtgtCCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCCCGCCAGGCt  <  2:139010/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CCAGATCGGTTGGATTCCCCACCGCCAGTAGCTGGCTCTGTTTATAGCGTTTAATCCCTTTGATGGTTTTGACGCTATCACGCGCTTTCTGCCATTCCGACAGCGGGATGCTGGCATAGACGCTAATACCGTGTTCTTCCAGTACTTGCGGGCTTTCAATGCCGCGATTAAACAACGAGCGCAGCAGCACACCCACGATAGAAAGCATCAGGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCT  >  NC_000913/2134137‑2134406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑