Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,301,060	A→G	F565S (TTC→TCC)	napA ←	periplasmic nitrate reductase subunit NapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,301,060	0	A	G	100.0%	56.0 / NA	17	F565S (TTC→TCC)	napA	periplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/12);  total (5/12)

AGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCA  >  NC_000913/2300928‑2301192                                                                                                                                     |                                                                                                                                     aGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGAc                                                                                                                               >  2:418855/1‑140 (MQ=255)  gTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCTACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAggactggact                                                                                                                              >  1:299135/1‑140 (MQ=255)  gTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAggactggact                                                                                                                              <  2:553028/140‑1 (MQ=255)     cGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAAc                                                                                                                           <  1:361461/140‑1 (MQ=255)             gggAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCagag                                                                                                                   >  2:752764/1‑140 (MQ=255)              ggAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCACCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAgaga                                                                                                                  >  1:67768/1‑140 (MQ=255)                          cTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATttcgct                                                                                                      >  2:794464/1‑140 (MQ=255)                                gtgtGGCATACAGCACTTCGTACAGCGTTTTGCTACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGccc                                                                                                <  2:299135/140‑1 (MQ=255)                                gtgtGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGccc                                                                                                >  1:672899/1‑140 (MQ=255)                                gtgtGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGccc                                                                                                <  2:528876/140‑1 (MQ=255)                                 tgtgGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCg                                                                                               <  2:672899/140‑1 (MQ=255)                                                          gTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGaa                                                                      <  1:752764/140‑1 (MQ=255)                                                                 cACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTa                                                               <  1:794464/140‑1 (MQ=255)                                                                        ttCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTAcggcgtt                                                        >  1:490748/1‑140 (MQ=255)                                                                        ttCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTAcggcgtt                                                        >  1:794798/1‑140 (MQ=255)                                                                                  ttcGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGGGGGCTGGACTAACTCCCCGAGATCCGATTTCGCTTCGCCCCGGGCCTGTACCTGTTGACGCCAGGACTGAGTACGGCGTTCGGCGTTAcc                                              >  2:298024/1‑140 (MQ=255)                                                                                         gcagATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAgcg                                       <  1:454720/140‑1 (MQ=255)                                                                                         gcagATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAgcg                                       <  1:418855/140‑1 (MQ=255)                                                                                         gcagATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAgcg                                       <  2:794798/140‑1 (MQ=255)                                                                                            gATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGccc                                    <  1:106348/140‑1 (MQ=255)                                                                                             aTCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCct                                   <  2:542108/140‑1 (MQ=255)                                                                                              tCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCctc                                  <  2:67768/140‑1 (MQ=255)                                                                                                                    ttttGAAGCGGCGGGAGGGCCGGGCCAACTGCCAGAGATCCGATTTCGCTTCGCCCGGGGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTc            >  2:520936/1‑140 (MQ=255)                                                                                                                              gcggGAGGACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCa  >  1:864368/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     AGTTCGGATACCGGGAATTTGCTCACTTCCGGTGTGGCATACAGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCA  >  NC_000913/2300928‑2301192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑