Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,956	C→T	G74D (GGC→GAC)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,956	0	C	T	100.0%	194.6 / NA	55	G74D (GGC→GAC)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/30);  total (25/30)

TAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACAT  >  NC_000913/2481823‑2482086                                                                                                                                      |                                                                                                                                   tAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTaa                                                                                                                              <  1:414774/140‑1 (MQ=255)  aaCCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAAc                                                                                                                             <  2:26944/140‑1 (MQ=255)               ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAGAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATg                                                                                                                >  2:588573/1‑140 (MQ=255)               ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATg                                                                                                                >  2:111551/1‑140 (MQ=255)               ctctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATg                                                                                                                >  2:274672/1‑140 (MQ=255)                 ctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGc                                                                                                              <  1:862872/140‑1 (MQ=255)                 ctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGc                                                                                                              <  2:663760/140‑1 (MQ=255)                 ctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGc                                                                                                              <  2:321957/140‑1 (MQ=255)                 ctAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGc                                                                                                              <  1:274672/140‑1 (MQ=255)                  tAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCa                                                                                                             <  2:111424/140‑1 (MQ=255)                         tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTa                                                                                                      >  2:35095/1‑140 (MQ=255)                         tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCCTTGTGCCAACCTGTCAGTAACAGGGATCGCa                                                                                                                   >  2:570535/1‑127 (MQ=255)                         tatCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCCTTGTGCCAACCTGTCAGTAACAGGGATCGCa                                                                                                                   <  1:570535/127‑1 (MQ=255)                                  aTCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACg                                                                                             <  1:212267/140‑1 (MQ=255)                                   tCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGa                                                                                            <  1:308177/140‑1 (MQ=255)                                    cAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAg                                                                                           >  2:682193/1‑140 (MQ=255)                                         ttGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGAt                                                                                      <  1:35095/140‑1 (MQ=255)                                         ttGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGAt                                                                                      >  2:637196/1‑140 (MQ=255)                                          tGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATa                                                                                     <  1:202858/140‑1 (MQ=255)                                            gTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGGGATAAc                                                                                   >  2:3989/1‑140 (MQ=255)                                                  aGGCTACACATTAATGAAGGCAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTCAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGt                                                                             >  1:786384/1‑140 (MQ=255)                                                  aGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGt                                                                             <  2:642153/140‑1 (MQ=255)                                                      tACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCt                                                                         >  2:273378/1‑140 (MQ=255)                                                             tAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTg                                                                  >  2:640844/1‑140 (MQ=255)                                                                    gACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGc                                                           >  2:576089/1‑140 (MQ=255)                                                                     gcAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTCAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCt                                                          <  2:786384/139‑1 (MQ=255)                                                                     aCAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCt                                                          >  2:746610/1‑140 (MQ=255)                                                                     aCAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCt                                                          >  2:175806/1‑140 (MQ=255)                                                                       aGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTcc                                                        >  2:174247/1‑140 (MQ=255)                                                                       aGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTcc                                                        <  1:637196/140‑1 (MQ=255)                                                                         cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTc                                                                    >  2:294663/1‑126 (MQ=255)                                                                         cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTc                                                                    <  1:294663/126‑1 (MQ=255)                                                                         cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAACGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCt                                                          >  1:45025/1‑136 (MQ=255)                                                                         cGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAACGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCt                                                          <  2:45025/136‑1 (MQ=255)                                                                           aaaaaaaaGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGa                                                    <  1:588573/140‑1 (MQ=255)                                                                              aaaaaGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAg                                                 <  1:576089/140‑1 (MQ=255)                                                                               aaaaGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGc                                                <  1:174247/140‑1 (MQ=255)                                                                                     gACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTG‑TGCTCCCAGAAAGCCAg                                             >  2:210225/1‑136 (MQ=255)                                                                                     gACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTG‑TGCTCCCAGAAAGCCAg                                             <  1:210225/136‑1 (MQ=255)                                                                                      aCTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGatat                                         <  2:466452/140‑1 (MQ=255)                                                                                                   ataatCTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCg                            <  1:397768/140‑1 (MQ=255)                                                                                                        cTTAATTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGtt                       >  2:247275/1‑140 (MQ=255)                                                                                                            aTTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGtt                       <  1:762022/136‑1 (MQ=255)                                                                                                            aTTCGCCTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGtt                       >  2:762022/1‑136 (MQ=255)                                                                                                                 ccTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGt              >  2:635291/1‑140 (MQ=255)                                                                                                                 ccTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGC‑ATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTg             <  2:440478/140‑1 (MQ=255)                                                                                                                  cTATTCTTTGTGCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTg             <  2:753732/140‑1 (MQ=255)                                                                                                                          tgtgCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGt              >  1:863870/1‑131 (MQ=255)                                                                                                                          tgtgCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGt              <  2:863870/131‑1 (MQ=255)                                                                                                                          tgtgCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCaa     >  1:521829/1‑140 (MQ=255)                                                                                                                          tgtgCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCaa     >  2:810615/1‑140 (MQ=255)                                                                                                                             gCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACAt  <  2:608941/140‑1 (MQ=255)                                                                                                                             gCCAACCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACAt  <  1:640844/140‑1 (MQ=255)                                                                                                                                 aCCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGt              <  1:863565/124‑1 (MQ=255)                                                                                                                                 aCCTGTCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGt              >  2:863565/1‑124 (MQ=255)                                                                                                                                      |                                                                                                                                   TAACCCCTGAACGACTCTAAAAAATATCAGCACATCAAGATTGGTCGATAGGCTACACATTAATGAAGACAGCGAAAAAAAAGTGACTGAAAGTAAAAATAATCTTAATTCGCCTATTCTTTGTGCCAACCTGCCAGTAACAGGGATCGCAATGGCATTTGCTACACCAAACGAGGTGATAACCCAGGTGCCTTCGTCTGTTGATGCTCCCAGAAAGCCAGATATTGTCGGTATTGCGACGTTAGAAATAGTGGAATCCAACAT  >  NC_000913/2481823‑2482086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑