Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,528,483	G→A	A565V (GCC→GTC)	ligA ←	DNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,528,483	0	G	A	100.0%	92.9 / NA	27	A565V (GCC→GTC)	ligA	DNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/13);  total (14/13)

GGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCG  >  NC_000913/2528346‑2528617                                                                                                                                          |                                                                                                                                       ggCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACa                                                                                                                                      <  1:78961/140‑1 (MQ=255)       aGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAaagaag                                                                                                                                >  2:259212/1‑140 (MQ=255)            cccGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTtgtg                                                                                                                           <  1:8409/140‑1 (MQ=255)            cccGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTtgtg                                                                                                                           >  2:826872/1‑140 (MQ=255)            cccGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTtgtg                                                                                                                           >  1:64594/1‑140 (MQ=255)             ccGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGa                                                                                                                          <  2:282382/140‑1 (MQ=255)                tAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACg                                                                                                                       <  1:259212/140‑1 (MQ=255)                    caccacGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTggg                                                                                                                   <  1:323951/140‑1 (MQ=255)                     accacGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGa                                                                                                                  <  2:378974/140‑1 (MQ=255)                      ccacGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATCGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGAt                                                                                                                 >  2:407102/1‑140 (MQ=255)                          ggTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCaa                                                                                                             >  2:457498/1‑140 (MQ=255)                              ttACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGAc                                                                                                         <  1:864945/140‑1 (MQ=255)                                            gCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAg                                                                                           <  2:479710/140‑1 (MQ=255)                                                       ctTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGc                                                                                <  2:64594/140‑1 (MQ=255)                                                          ccGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGc                                                                             >  1:151835/1‑140 (MQ=255)                                                                       gATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAg                                                                <  1:267090/140‑1 (MQ=255)                                                                            gcgcAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgct                                                           >  1:443096/1‑140 (MQ=255)                                                                               cAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATCGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttcc                                                        <  1:407102/140‑1 (MQ=255)                                                                                 ggCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccag                                                      <  1:142095/140‑1 (MQ=255)                                                                                 ggCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGgcttccag                                                      <  1:826872/140‑1 (MQ=255)                                                                                                tCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGcc                                       >  2:207771/1‑140 (MQ=255)                                                                                                tCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGcc                                       >  2:527087/1‑140 (MQ=255)                                                                                                      aaCAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGCCGAAata                                 >  1:264952/1‑140 (MQ=255)                                                                                                             cGCTGATGACATTGCGGTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCC‑ACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCa                         >  1:807572/1‑140 (MQ=255)                                                                                                                            ggTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTg           >  2:286825/1‑140 (MQ=255)                                                                                                                             gTTGCTTTCTTCGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTgg          >  2:453106/1‑140 (MQ=255)                                                                                                                                     cttcGACAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCg  >  1:287496/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATCGGCGCAGGCCAGTGAACACCTTCCGCCAACAGCTCGCTGATGACATTGCGGTTGCTTTCTTCGGCAAAGAAGTTGTGAACGTGGGATGCAACGACAATGCCAACATCAGGCACCTTTTGCAGCTCTTCAATCGAAGCGGCTTCCAGCGCTTCCAGCGTGCCGAAATATGCCGCCAGACCTGCTGCGGTGGCCTCGCCG  >  NC_000913/2528346‑2528617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑