Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,555,795	G→A	N202N (AAC→AAT)	eutL ←	putative structural protein, ethanolamine utilization microcompartment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,555,795	0	G	A	100.0%	92.6 / NA	27	N202N (AAC→AAT)	eutL	putative structural protein, ethanolamine utilization microcompartment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

GCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCT  >  NC_000913/2555659‑2555930                                                                                                                                         |                                                                                                                                        gCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTa                                                                                                                                      >  2:573996/1‑140 (MQ=255)     ggCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAgg                                                                                                                                  >  1:844063/1‑140 (MQ=255)     ggCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAgg                                                                                                                                  <  2:272923/140‑1 (MQ=255)     ggCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCAAGCACTGCATCGGTAAAGGCATTACAgg                                                                                                                                  <  2:644335/140‑1 (MQ=255)                     aTCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGc                                                                                                                  >  2:552688/1‑140 (MQ=255)                            cgACCATGCGGTCCACTTCCAGAAATCCCAGGGCATTGTTCATCGGCAACCTCCGTTACGCACCCTGGATTGGTTTAGGGCCGATTTCCACCACGCCATGGGAAAGGCCATTACAGGCGGCTTTAAAGGCGGCCTGGcaa                                                                                                           >  2:813442/1‑138 (MQ=255)                            cgACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTa                                                                                                           <  1:790893/140‑1 (MQ=255)                             gACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTAc                                                                                                          <  2:71085/140‑1 (MQ=255)                             gACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTAc                                                                                                          <  1:98597/140‑1 (MQ=255)                               ccATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCg                                                                                                        <  1:552688/140‑1 (MQ=255)                                           cTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAtgct                                                                                            <  2:179737/140‑1 (MQ=255)                                            ttCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAtgctg                                                                                           >  2:656337/1‑140 (MQ=255)                                            ttCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAtg                                                                                              <  1:132036/137‑1 (MQ=255)                                            ttCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAAtg                                                                                              >  2:132036/1‑137 (MQ=255)                                                    aTCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGagtag                                                                                   >  1:160187/1‑140 (MQ=255)                                                     tCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGagtagt                                                                                  <  1:813442/140‑1 (MQ=255)                                                     tCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGagtagt                                                                                  >  1:784008/1‑140 (MQ=255)                                                      cccAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGtt                                                                                 >  2:88189/1‑140 (MQ=255)                                                                            aCCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACa                                                           >  1:545230/1‑140 (MQ=255)                                                                             ccTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACAt                                                          <  2:519793/140‑1 (MQ=255)                                                                             ccTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACAt                                                          <  1:400707/140‑1 (MQ=255)                                                                                   ttACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCg                                                    <  1:88189/140‑1 (MQ=255)                                                                                                    gattACGCGCGATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCa                                   <  2:610381/140‑1 (MQ=255)                                                                                                         cgcgcgATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACtt                              >  2:580679/1‑140 (MQ=255)                                                                                                           cgcgATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCa                            >  2:462673/1‑140 (MQ=255)                                                                                                              gATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCgg                                     <  2:536055/128‑1 (MQ=255)                                                                                                              gATTTCCAGCACTGCATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCgg                                     >  1:536055/1‑128 (MQ=255)                                                                                                                            cATCGGTAAAGGCATTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCa           >  1:25257/1‑140 (MQ=255)                                                                                                                                     aGGCGTTACAGGCTGCTTTACACGCTGCCTGGATAGCGGTTAAAAATGATGCCGAGTAGTTGGTTTTAGAAGGTGGAGGGACATAGGTCACCAGCTGTTCGTCGGCAGACTTCAGGGCTGCATCAATGCCATTGGtacct  <  2:584010/140‑5 (MQ=255)                                                                                                                                         |                                                                                                                                        GCATGGCATCCGCTGCATCTATCGCGGCGACCATGCCGTCCACTTCCAGCAATCCCAGTGCATTGATCATCGGCAACCTCCGTTACGCACGCTGGATTGGATTACGCGCGATTTCCAGCACTGCATCGGTAAAGGCGTTACAGGCTGCTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCT  >  NC_000913/2555659‑2555930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑