Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,583,930	A→G	L532L (CTT→CTC)	aegA ←	putative oxidoreductase Fe‑S subunit AegA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,583,930	0	A	G	100.0%	105.4 / NA	30	L532L (CTT→CTC)	aegA	putative oxidoreductase Fe‑S subunit AegA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

CAAATTCACTGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGACGTTACTCGCGCCGTGGCGC  >  NC_000913/2583818‑2584062                                                                                                                 |                                                                                                                                     cAAATTCACTGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCg                                                                                                           >  2:830053/1‑140 (MQ=255)  aaaTTCACTGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGa                                                                                                          <  2:539985/140‑1 (MQ=255)    aTTCACTGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAg                                                                                                        <  1:779925/140‑1 (MQ=255)    aTTCACTGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAg                                                                                                        <  2:468644/140‑1 (MQ=255)     ttCACTGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGt                                                                                                       >  1:852869/1‑140 (MQ=255)          tGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGcc                                                                                                  >  1:741455/1‑140 (MQ=255)          tGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGcc                                                                                                  >  1:87948/1‑140 (MQ=255)                      gCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCgg                                                                                      >  2:658735/1‑140 (MQ=255)                       cACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCggg                                                                                     <  1:106838/140‑1 (MQ=255)                         cTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCg                                                                                   >  1:473127/1‑140 (MQ=255)                         cTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCg                                                                                   <  1:398764/140‑1 (MQ=255)                            gacgccgacgccCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGttc                                                                                >  2:621798/1‑140 (MQ=255)                                          tGGGCATCCGGCTCTCTAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCGGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTtctttctt                                                                  <  2:771119/140‑1 (MQ=255)                                          tGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTtctttctt                                                                  <  1:830053/140‑1 (MQ=255)                                              cATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAg                                                              <  2:741455/140‑1 (MQ=255)                                              cATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAg                                                              <  2:87948/140‑1 (MQ=255)                                                 ccGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCt                                                           <  1:621798/140‑1 (MQ=255)                                                 ccGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCt                                                           <  1:658735/140‑1 (MQ=255)                                                      tctcCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAg                                                              >  1:98386/1‑132 (MQ=255)                                                      tctcCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAg                                                              <  2:98386/132‑1 (MQ=255)                                                          cAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTa                                                  >  2:824337/1‑140 (MQ=255)                                                            aGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGc                                                <  1:536624/140‑1 (MQ=255)                                                                                 aTCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCa                           <  2:186750/140‑1 (MQ=255)                                                                                  tCCCGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAg                          <  2:835443/140‑1 (MQ=255)                                                                                    ccGCAGACGTGACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGt                        >  2:622321/1‑140 (MQ=255)                                                                                              gACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGACGTTACTc              >  2:376170/1‑140 (MQ=255)                                                                                              gACCTTGTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGACGTTACTc              >  1:32430/1‑140 (MQ=255)                                                                                                    gTTCATTCAGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGACGTTACTCGCGCCg        >  2:825439/1‑140 (MQ=255)                                                                                                         ttcaGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGACGTTACTCGCGCCGTGgcg   <  2:852869/140‑1 (MQ=255)                                                                                                          tcaGCTCGAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGACGTTACTCGCGCCGTGgcgc  >  1:246101/1‑140 (MQ=255)                                                                                                                 |                                                                                                                                     CAAATTCACTGCCTTCCACCGGCACTGGACGCCGACGCCCCTGGGCATCCGGCTCTCCAAGACGCGTGCGCAGGAAACGAATCCCGCAGACGTGACCTTGTTCATTCAGCTCAAGCGCCACCGGCTGGACGTTAAATTCGAAGTTGGCCCCCTCTTCGCGGGCGTTCTTCACTTCTTTCTTCGAGCCTGGCATGTTAGCTTCATCACGACGATAAGCGCAGGTGACGTTACTCGCGCCGTGGCGC  >  NC_000913/2583818‑2584062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑