Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	301,344	C→T	E182K (GAG→AAG)	paoB ←	aldehyde dehydrogenase, FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	301,344	0	C	T	100.0%	88.7 / NA	26	E182K (GAG→AAG)	paoB	aldehyde dehydrogenase, FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/4);  total (22/4)

ATATGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGCGCCG  >  NC_000913/301208‑301472                                                                                                                                          |                                                                                                                                 atatGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCgg                                                                                                                               >  1:204046/1‑140 (MQ=255) atatGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCgg                                                                                                                               >  1:812838/1‑140 (MQ=255) atatGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCgg                                                                                                                               >  2:776446/1‑140 (MQ=255) atatGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCgg                                                                                                                               >  2:622208/1‑140 (MQ=255) atatGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCgg                                                                                                                               >  1:574782/1‑140 (MQ=255)   atGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCg                                                                                                                             <  1:622208/140‑1 (MQ=255)          ccgccgAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGt                                                                                                                      >  1:381437/1‑140 (MQ=255)           cgccgAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGtt                                                                                                                     >  1:853753/1‑140 (MQ=255)                  cGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTccacca                                                                                                              >  1:355682/1‑140 (MQ=255)                        aGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCAt                                                                                                        <  2:796973/140‑1 (MQ=255)                                tCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAAc                                                                                                <  2:574782/140‑1 (MQ=255)                                 cACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAAcc                                                                                               >  1:849314/1‑140 (MQ=255)                                   ccGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGc                                                                                             >  1:289945/1‑140 (MQ=255)                                     acAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGc                                                                                             >  2:151586/2‑138 (MQ=255)                                     acAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGc                                                                                             <  1:151586/137‑1 (MQ=255)                                                 cccccGGGAAGCGGGGGGGTTTCAATG‑GGGGGGTTTTTCCCGGGAGGGTGAAAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTGGGCGTGATGTTTCCCCCCCCCGCACCCAGCAACCGCATTGCGACCGCCat                                                                               <  2:289945/137‑1 (MQ=255)                                                   aCCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCatat                                                                             >  1:775779/1‑140 (MQ=255)                                                       ggAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCt                                                                         >  1:157855/1‑140 (MQ=255)                                                                   gTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGc                                                             >  1:404803/1‑140 (MQ=255)                                                                            tgCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGc                                                    >  1:823107/1‑140 (MQ=255)                                                                            tgCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGc                                                    >  1:518710/1‑140 (MQ=255)                                                                            ggcggcgTTTTCCCC‑GGAGGGTGAAAAAAACCACCCGGTGTGATACTGCGAGTCTTCCCCTTGGGGGTGATGGTTCCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGc                                                    <  2:512766/139‑1 (MQ=255)                                                                              cggcgTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGcct                                                  >  1:427804/1‑138 (MQ=255)                                                                              cggcgTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCtt                                                  >  1:168660/1‑140 (MQ=255)                                                                                   ttttcCCG‑GGGGGGTAAAAAAAATCAGCCAGTGTGATACTGCGAGTCTTCCCCTTGGGGGTGATGTTTTCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCtt                                             <  2:381437/140‑1 (MQ=255)                                                                                   ttttcCCG‑GGAGGGTAAAAAAAATCAGCCAGGGTGATCCTGCGAGTCTTCCCCTTGGGCGTGATGTTTCCCCCCCCCGCATCCAGCAACCGTTTTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCtt                                             <  2:274077/140‑1 (MQ=255)                                                                                   ttttcCCC‑GGGGGGTAAAAAAAATCAGCCAGTGTGATCCTGGGTGTTTTCCCCTGCGGGGTGATGGTTCCCCCCCCCGCATCCACCACCCGCTTTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCtt                                             <  2:404803/140‑1 (MQ=255)                                                                                   ttttcCCC‑GGAGGGTAAAAAAAACCAGCGAGGGTGACGGTGCGGTTCTTCCCCTGCGGGGGGATTTTCTCCCCCCCCGCAGCAAGCACCCGCTGGGGGTCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCtt                                             <  2:812838/140‑1 (MQ=255)                                                                                      ttCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTTCGGCGTGATGG‑TTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGc                                         >  1:628460/1‑140 (MQ=255)                                                                                           gggggggTAAAAAAAACCGGCCGGTGTGATACTGCGATTTTTCCCCTGGGGCGTGTTGTTTCCCCCCCCCCCACCCACCACCCGCTTTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTa                                      <  2:849314/136‑1 (MQ=255)                                                                                                       aaaaTCAGCCAGTGTGATCCTGCGATTCTTCCCCTTGGGCGTGA‑GGTTCCCCCCCCCGCACCCAGCACCCGCATTGCGCCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTg                         <  2:628460/140‑1 (MQ=255)                                                                                                                             cGAGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGAGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAgcgc    >  1:89999/1‑140 (MQ=255)                                                                                                                               aGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGcgccg  >  1:337255/1‑140 (MQ=255)                                                                                                                               aGTCTTTCCCTTCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGAATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGcgccg  >  1:703826/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 ATATGTTTTCCGCCGAGCGGTGGAGGTAACGTCACCGCAACGATAAGCTCACCGGGAAGCAGGGCGGTTTCAATGTGCGGCGTTTTTCCC‑GGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTGACGGCTAAAGCCTTCAAGCGCCG  >  NC_000913/301208‑301472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑