Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,673,499 C→T P52S (CCA→TCA)  yphA → putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,4990CT100.0% 165.2 / NA 48P52S (CCA→TCA) yphAputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

TGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGCCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTG  >  NC_000913/2673366‑2673631
                                                                                                                                     |                                                                                                                                    
tgattttgGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGc                                                                                                                                >  1:153479/1‑140 (MQ=255)
       ggAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCt                                                                                                                         >  2:297466/1‑140 (MQ=255)
             gcccgcccCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGc                                                                                                                   <  2:153479/140‑1 (MQ=255)
                      gTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGc                                                                                                          <  2:735887/140‑1 (MQ=255)
                       ttttGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTTGCAGCGATTATTGCg                                                                                                         <  1:619471/140‑1 (MQ=255)
                       ttttGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCg                                                                                                         <  1:533658/140‑1 (MQ=255)
                         ttGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGt                                                                                                       <  2:82513/140‑1 (MQ=255)
                                      cccGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTg                                                                                          >  2:214679/1‑140 (MQ=255)
                                        cGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGcc                                                                                        >  2:124198/1‑140 (MQ=255)
                                        cGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGcc                                                                                        <  1:214679/140‑1 (MQ=255)
                                           aTCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGcc                                                                                        <  1:399847/137‑1 (MQ=255)
                                           aTCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGcc                                                                                        >  2:399847/1‑137 (MQ=255)
                                           aTCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCccgc                                                                                     >  1:489235/1‑140 (MQ=255)
                                            tCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCccgcc                                                                                    <  2:489235/140‑1 (MQ=255)
                                             cGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCccgccg                                                                                   <  2:545947/140‑1 (MQ=255)
                                              gCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCcgccgc                                                                                  <  2:698683/140‑1 (MQ=255)
                                              gCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCcgccgc                                                                                  <  2:676150/140‑1 (MQ=255)
                                                               cATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTg                                                                 >  2:49245/1‑140 (MQ=255)
                                                               cATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTg                                                                 >  1:85439/1‑140 (MQ=255)
                                                                            tttCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCg                                                    <  1:297466/140‑1 (MQ=255)
                                                                              tCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTc                                                  >  2:604181/1‑140 (MQ=255)
                                                                               cccAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTcc                                                 >  1:204597/1‑140 (MQ=255)
                                                                                    aaTGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTgg                                            <  2:85439/140‑1 (MQ=255)
                                                                                        atgGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGt                                        <  2:477962/140‑1 (MQ=255)
                                                                                         tgGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTg                                       <  1:743340/140‑1 (MQ=255)
                                                                                                tGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGc                                               <  1:459315/125‑1 (MQ=255)
                                                                                                tGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGc                                               >  2:459315/1‑125 (MQ=255)
                                                                                                      tacggtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCt                          >  1:222753/1‑140 (MQ=255)
                                                                                                             cAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCt                   <  2:222753/140‑1 (MQ=255)
                                                                                                                tatatGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTTTACACGCTggg                >  1:37747/1‑140 (MQ=255)
                                                                                                                tatatGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTa                         >  1:495139/1‑131 (MQ=255)
                                                                                                                tatatGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTa                         <  2:495139/131‑1 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTac                        >  1:57868/1‑127 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTac                        <  2:57868/127‑1 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGt               <  1:549313/136‑1 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGt               >  2:549313/1‑136 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcgg           >  2:57063/1‑140 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcgg           >  1:364150/1‑140 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcgg           >  1:66273/1‑140 (MQ=255)
                                                                                                                     ggCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcgg           >  1:727439/1‑140 (MQ=255)
                                                                                                                       ccTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGt               >  1:387503/1‑134 (MQ=255)
                                                                                                                       ccTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGt               <  2:387503/134‑1 (MQ=255)
                                                                                                                       ccTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcggcg         >  1:773883/1‑140 (MQ=255)
                                                                                                                           gTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcggc          <  2:291049/135‑1 (MQ=255)
                                                                                                                           gTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcggc          >  1:291049/1‑135 (MQ=255)
                                                                                                                           gTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGa     <  1:604181/140‑1 (MQ=255)
                                                                                                                            ttGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGAt    <  1:124198/140‑1 (MQ=255)
                                                                                                                              gggCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTg  <  2:37747/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
TGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGCCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTG  >  NC_000913/2673366‑2673631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑