Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,693	T→C	D494G (GAC→GGC)	clpB ←	ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,693	0	T	C	100.0%	136.1 / NA	38	D494G (GAC→GGC)	clpB	ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/17);  total (21/17)

GCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCT  >  NC_000913/2732556‑2732829                                                                                                                                          |                                                                                                                                         gCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGccc                                                                                                                                        <  1:573779/140‑1 (MQ=255)  cACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGcccc                                                                                                                                       >  2:91471/1‑140 (MQ=255)             tttCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACgg                                                                                                                               <  1:284219/137‑1 (MQ=255)             tttCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACgg                                                                                                                               >  2:284219/1‑137 (MQ=255)                          cACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAg                                                                                                               <  2:423882/140‑1 (MQ=255)                          cACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCCTTTCAATAg                                                                                                               <  2:359110/140‑1 (MQ=255)                                  taCGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTc                                                                                                       <  2:540728/140‑1 (MQ=255)                                     gCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGcc                                                                                                    <  2:339463/140‑1 (MQ=255)                                       aaCAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTg                                                                                                  >  1:61679/1‑140 (MQ=255)                                         cAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAg                                                                                                               >  1:459023/1‑125 (MQ=255)                                         cAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAg                                                                                                               <  2:459023/125‑1 (MQ=255)                                            aCGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCa                                                                                             >  1:511690/1‑140 (MQ=255)                                                aTAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTc                                                                                         <  2:123706/140‑1 (MQ=255)                                                  aGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCg                                                                                       <  2:511690/140‑1 (MQ=255)                                                       tGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTa                                                                                  >  2:837690/1‑140 (MQ=255)                                                       tGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTa                                                                                  <  1:238619/140‑1 (MQ=255)                                                       tGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGA‑TTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTaa                                                                                 >  2:63862/1‑140 (MQ=255)                                                         ccTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAAt                                                                                >  2:559293/1‑140 (MQ=255)                                                            tCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGt                                                                             <  2:681622/140‑1 (MQ=255)                                                                    cGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTAc                                                                     >  1:669741/1‑140 (MQ=255)                                                                         cGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGaa                                                                >  2:817823/1‑140 (MQ=255)                                                                         cGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGaa                                                                >  2:463689/1‑140 (MQ=255)                                                                               ccAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGAt                                                          <  1:837690/140‑1 (MQ=255)                                                                               ccAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGAt                                                          <  1:91471/140‑1 (MQ=255)                                                                                        tttCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTctct                                                 <  2:362992/140‑1 (MQ=255)                                                                                        tttCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCATTctct                                                 >  1:586529/1‑140 (MQ=255)                                                                                              gTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTc                                           <  2:77986/140‑1 (MQ=255)                                                                                                  cGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCAct                                       <  1:464462/140‑1 (MQ=255)                                                                                                    ggATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTtct                                                   <  1:512154/126‑1 (MQ=255)                                                                                                    ggATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTtct                                                   >  2:512154/1‑126 (MQ=255)                                                                                                         ttGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACtcttcttc                                >  1:145750/1‑140 (MQ=255)                                                                                                         ttGCCGTATTGCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGAGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACtcttcttc                                >  2:592980/1‑140 (MQ=255)                                                                                                                   gCAGTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAg                      <  1:559293/140‑1 (MQ=255)                                                                                                                      gTTCAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTAc                   >  2:670414/1‑140 (MQ=255)                                                                                                                         cAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGa                >  1:59851/1‑140 (MQ=255)                                                                                                                         cAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGa                >  1:485641/1‑140 (MQ=255)                                                                                                                         cAGACATCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGa                >  2:409296/1‑140 (MQ=255)                                                                                                                              aTCCGCGCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTc           >  1:737886/1‑140 (MQ=255)                                                                                                                                 cgcgCCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCGTCTAACTCGGAGTACTGACGTTCttt        >  1:601624/1‑140 (MQ=255)                                                                                                                                   cgcCAGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGt      >  1:162474/1‑140 (MQ=255)                                                                                                                                       aGGCCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCt  <  1:817823/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCACTTCAGCAATTTCGGCGTCGGTCACTTTATTACGCAACAGACGCATAGTTTTGCCTTCGAGCTGCGTTGCGGCTTCCAGTTGCTTTTCCAGTTCCGGGATTTTGCCGTATTGCAGTTCAGACATCCGCGCCAGGTCCCCCACACGGCGAGCCTGTTCAATAGCGATTTTCGCCTGTTCCAGTTCCGCTTTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCT  >  NC_000913/2732556‑2732829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑