Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,833,404	T→C	V310A (GTC→GCC)	norV →	anaerobic nitric oxide reductase flavorubredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,833,404	0	T	C	100.0%	115.0 / NA	33	V310A (GTC→GCC)	norV	anaerobic nitric oxide reductase flavorubredoxin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

CACCCGCATGATGGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGTCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAGCGGCGGTGCGGTGGAT  >  NC_000913/2833267‑2833537                                                                                                                                          |                                                                                                                                      cacCCGCATGATGGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCg                                                                                                                                     >  2:295492/1‑140 (MQ=255)       cATGATGGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACtt                                                                                                                               <  1:242179/140‑1 (MQ=255)       cATGATGGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACtt                                                                                                                               <  2:617689/140‑1 (MQ=255)       cATGATGGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACtt                                                                                                                               <  1:854227/140‑1 (MQ=255)           atgGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTcgac                                                                                                                           <  2:839590/140‑1 (MQ=255)            tgGCTGACGCTATCGCCCAGGGGATTGCGGAAACCTACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTcgacg                                                                                                                          <  2:593535/140‑1 (MQ=255)            tgGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTcgacg                                                                                                                          <  1:800151/140‑1 (MQ=255)              gCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGAt                                                                                                                        <  1:676785/140‑1 (MQ=255)              gCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGAt                                                                                                                        <  1:75340/140‑1 (MQ=255)                 gACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGaa                                                                                                                     <  1:581511/140‑1 (MQ=255)                                     tGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGaaaa                                                                                                 >  1:714436/1‑140 (MQ=255)                                     tGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGaaaa                                                                                                 >  1:577957/1‑140 (MQ=255)                                         gAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGc                                                                                             <  2:714436/140‑1 (MQ=255)                                            aCCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCgg                                                                                          <  2:156863/140‑1 (MQ=255)                                              cGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGc                                                                                        <  2:187210/140‑1 (MQ=255)                                              cGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGc                                                                                        <  1:670899/140‑1 (MQ=255)                                                         tGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGgaggag                                                                             <  1:816123/140‑1 (MQ=255)                                                                gAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTg                                                                      >  1:419976/1‑140 (MQ=255)                                                                gAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTg                                                                      >  1:715784/1‑140 (MQ=255)                                                                        tCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACg                                                               >  1:400311/1‑139 (MQ=255)                                                                        tCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACg                                                               <  2:400311/139‑1 (MQ=255)                                                                         cAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGAc                                                                        <  2:359405/129‑1 (MQ=255)                                                                         cAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGAGGAGGAGATGAc                                                                        >  1:359405/1‑129 (MQ=255)                                                                                                       ccTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGct                               <  2:577957/140‑1 (MQ=255)                                                                                                         tGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGctct                             >  1:226126/1‑140 (MQ=255)                                                                                                           aCTAATGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCa                           <  2:715784/140‑1 (MQ=255)                                                                                                                tGTCTTCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCt                      >  2:719079/1‑140 (MQ=255)                                                                                                                    ttCCGCTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAg                  <  2:419976/140‑1 (MQ=255)                                                                                                                         cTCAAAAGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAgcggcg             >  2:813099/1‑140 (MQ=255)                                                                                                                            aaaaGGCGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAGCGgcggtg          <  1:719079/140‑1 (MQ=255)                                                                                                                                  cGTGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAGCGGCGGTGCGGTgg    >  1:851429/1‑140 (MQ=255)                                                                                                                                    tGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAGCGGCGGTGCGgggggg  >  2:630919/1‑138 (MQ=255)                                                                                                                                    tGCTGGCCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAGCGGCGGTGCGGTGGAt  >  1:585015/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      CACCCGCATGATGGCTGACGCTATCGCCCAGGGGATTGCGGAAACCGACCCACGCGTGGCGGTGAAAATTTTCAACGTCGCCCGAAGCGATAAAAACGAAATCCTGACTAATGTCTTCCGCTCAAAAGGCGTGCTGGTCGGCACTTCGACGATGAATAACGTGATGATGCCGAAAATCGCCGGGCTGGTGGAGGAGATGACTGGTTTACGCTTCCGTAACAAACGCGCCAGTGCTTTCGGCTCTCACGGCTGGAGCGGCGGTGCGGTGGAT  >  NC_000913/2833267‑2833537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑