Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,840,022	G→A	A167T (GCA→ACA)	ascF →	beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,840,022	0	G	A	100.0%	172.0 / NA	49	A167T (GCA→ACA)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/24);  total (25/24)

GGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCG  >  NC_000913/2839890‑2840156                                                                                                                                     |                                                                                                                                       ggcggATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTgc                                                                                                                                 >  1:472821/1‑140 (MQ=255) ggcggATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTgc                                                                                                                                 <  1:439741/140‑1 (MQ=255)      aTCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCa                                                                                                                            >  1:345070/1‑140 (MQ=255)        cGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATc                                                                                                                          <  1:334165/140‑1 (MQ=255)                aaaCTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTaa                                                                                                                  <  2:175996/140‑1 (MQ=255)                   ctgctgGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAAc                                                                                                               <  1:82544/140‑1 (MQ=255)                              gATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGc                                                                                                    >  1:179953/1‑140 (MQ=255)                                   tCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCg                                                                                               >  2:166337/1‑140 (MQ=255)                                           aGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTAGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCAt                                                                                       >  2:489680/1‑140 (MQ=255)                                              ggcgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATcgc                                                                                    <  2:179953/140‑1 (MQ=255)                                                cgTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCgg                                                                                  <  2:472821/140‑1 (MQ=255)                                                 gTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCggg                                                                                 <  2:510125/140‑1 (MQ=255)                                                    cTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGgtgt                                                                              <  2:513856/140‑1 (MQ=255)                                                     tGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGtgtg                                                                             <  2:35299/140‑1 (MQ=255)                                                        cAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTg                                                                          <  1:166337/140‑1 (MQ=255)                                                                tCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTAGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATcc                                                                  <  1:489680/140‑1 (MQ=255)                                                                 cGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTACCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCgg                                                                                  <  2:375647/123‑1 (MQ=255)                                                                 cGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTACCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCgg                                                                                  >  1:375647/1‑123 (MQ=255)                                                                   ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg                                                               <  1:460502/140‑1 (MQ=255)                                                                   ccGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAg                                                               <  2:452807/140‑1 (MQ=255)                                                                         ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt                                                             >  1:71255/1‑136 (MQ=255)                                                                         ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt                                                             <  2:71255/136‑1 (MQ=255)                                                                         ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt                                                             <  2:204861/136‑1 (MQ=255)                                                                         ttAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGtt                                                             >  1:204861/1‑136 (MQ=255)                                                                                 tCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCTCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGa                                                 >  2:353322/1‑140 (MQ=255)                                                                                  cTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGAt                                                >  2:59130/1‑140 (MQ=255)                                                                                     aaTGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGc                                             <  2:460840/140‑1 (MQ=255)                                                                                         tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa                                         <  1:59130/140‑1 (MQ=255)                                                                                         tgATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGaaa                                         <  1:827966/140‑1 (MQ=255)                                                                                          gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGaa                                                     <  1:43776/127‑1 (MQ=255)                                                                                          gATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGaa                                                     >  2:43776/1‑127 (MQ=255)                                                                                           aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGc                                       >  1:173000/1‑140 (MQ=255)                                                                                           aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGc                                       >  1:654366/1‑140 (MQ=255)                                                                                           aTTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAAGATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGc                                       >  2:598838/1‑140 (MQ=255)                                                                                               gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGcc                                   >  2:798079/1‑140 (MQ=255)                                                                                               gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGcc                                   <  2:470278/140‑1 (MQ=255)                                                                                               gTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGcc                                   >  1:470278/1‑140 (MQ=255)                                                                                                   cGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAgg                               >  2:109888/1‑140 (MQ=255)                                                                                                     gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGcc                                   <  2:853011/134‑1 (MQ=255)                                                                                                     gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGcc                                   <  2:731576/134‑1 (MQ=255)                                                                                                     gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGcc                                   >  1:731576/1‑134 (MQ=255)                                                                                                     gTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGcc                                   >  1:853011/1‑134 (MQ=255)                                                                                                        cTTTCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGaa                          >  2:425955/1‑140 (MQ=255)                                                                                                         tttCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAAc                         >  1:227629/1‑140 (MQ=255)                                                                                                         tttCTTCTTCCTGCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAAc                         <  2:399/140‑1 (MQ=255)                                                                                                                     gCCGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTg             >  2:336406/1‑140 (MQ=255)                                                                                                                       cGCTGATGGTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGcc           >  2:483792/1‑140 (MQ=255)                                                                                                                              ggTCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGACATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCGGGGTGAACATGTCGAATTTGCCCTGATTc    >  1:552268/1‑140 (MQ=255)                                                                                                                                tCGCGACATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCg  >  1:316465/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       GGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTCCTGCCGCTGATGGTCGCGGCATCTGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCTGGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCG  >  NC_000913/2839890‑2840156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑