Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,880,582	G→A	P156L (CCG→CTG)	casD ←	type I‑E CRISPR system Cascade subunit CasD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,880,582	0	G	A	100.0%	155.8 / NA	44	P156L (CCG→CTG)	casD	type I‑E CRISPR system Cascade subunit CasD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

GTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCA  >  NC_000913/2880451‑2880710                                                                                                                                    |                                                                                                                                 gctcgtcaCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCCCCAACGTGCTCATAATTTAATAACGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGtt                                                                                                                          >  2:843313/3‑140 (MQ=255)     gtcgCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTg                                                                                                                      >  2:378231/1‑140 (MQ=255)                          atgatgCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGt                                                                                                 <  2:855127/140‑1 (MQ=255)                          atgatgCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGt                                                                                                 <  1:540965/140‑1 (MQ=255)                              tgCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAgg                                                                                             >  1:808518/1‑140 (MQ=255)                               gCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGgt                                                                                            >  1:571662/1‑140 (MQ=255)                                      aaCTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATAcc                                                                                     <  1:378231/140‑1 (MQ=255)                                        cTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGa                                                                                   <  2:294009/140‑1 (MQ=255)                                           aTTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGc                                                                                >  2:131516/1‑140 (MQ=255)                                               cTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTa                                                                            >  1:730187/1‑140 (MQ=255)                                               cTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTa                                                                            >  2:644874/1‑140 (MQ=255)                                                 cACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAAt                                                                          >  2:124878/1‑140 (MQ=255)                                                           atCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCttttt                                                                >  2:49494/1‑140 (MQ=255)                                                              gccgccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCaa                                                             >  2:726117/1‑140 (MQ=255)                                                              gccgccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCaa                                                             >  2:700744/1‑140 (MQ=255)                                                              gccgccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCaa                                                             >  2:472009/1‑140 (MQ=255)                                                                 gccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGtt                                                          <  2:230729/140‑1 (MQ=255)                                                                  ccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGTTGCCTGACATGTCCCC‑AAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTg                                                                      <  1:59516/126‑1 (MQ=255)                                                                  ccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGTTGCCTGACATGTCCCC‑AAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTg                                                                      >  2:59516/1‑126 (MQ=255)                                                                  ccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATAACGAGGCTTTAATACTGCTTTTTCAAGTTc                                                         >  2:270796/1‑140 (MQ=255)                                                                           tCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAAc                                                <  1:644874/140‑1 (MQ=255)                                                                             aTAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCa                                              <  1:49494/140‑1 (MQ=255)                                                                              tAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCAt                                             <  1:472009/140‑1 (MQ=255)                                                                                  ttAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGtt                                         <  1:726117/140‑1 (MQ=255)                                                                                       aGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGtt                                         >  1:213034/1‑135 (MQ=255)                                                                                       aGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGtt                                         <  2:213034/135‑1 (MQ=255)                                                                                       aGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATg                                    <  2:273681/140‑1 (MQ=255)                                                                                       aGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATg                                    >  1:631446/1‑140 (MQ=255)                                                                                         cgcCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATggg                                  <  1:432301/140‑1 (MQ=255)                                                                                         cgcCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATggg                                  >  1:250148/1‑140 (MQ=255)                                                                                         cgcCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATggg                                  >  2:629201/1‑140 (MQ=255)                                                                                         cgcCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATggg                                  <  1:35690/140‑1 (MQ=255)                                                                                         cgcCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATggg                                  >  1:670264/1‑140 (MQ=255)                                                                                         cgcCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATg                                    <  1:569484/138‑1 (MQ=255)                                                                                         cgcCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATg                                    >  2:569484/1‑138 (MQ=255)                                                                                           ccTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGgt                                <  1:124878/140‑1 (MQ=255)                                                                                           ccTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGgt                                >  1:752658/1‑140 (MQ=255)                                                                                           ccTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGgt                                >  1:457977/1‑140 (MQ=255)                                                                                           ccTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATAACGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGgt                                <  1:270796/140‑1 (MQ=255)                                                                                                          aTGCCTGACATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCg                 <  1:629201/140‑1 (MQ=255)                                                                                                                 aCATGTCCCCAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTaa          >  1:716317/1‑140 (MQ=255)                                                                                                                       ccccAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAaggagg    >  1:214874/1‑140 (MQ=255)                                                                                                                        cccAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGc   >  1:589528/1‑140 (MQ=255)                                                                                                                         ccAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCa  <  2:466347/140‑1 (MQ=255)                                                                                                                         ccAAAAAAAGCAGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCa  <  2:631446/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 GTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCA  >  NC_000913/2880451‑2880710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑