Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	311,430	A→G	intergenic (‑94/‑682)	ecpR ← / → ykgL	DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	311,430	0	A	G	100.0%	182.9 / NA	51	intergenic (‑94/‑682)	ecpR/ykgL	DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/18);  total (33/18)

TTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAACGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGCTTTTTTGA  >  NC_000913/311295‑311564                                                                                                                                        |                                                                                                                                       tttAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCgag                                                                                                                                    >  1:43817/1‑140 (MQ=255) tttAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCgag                                                                                                                                    >  2:723337/1‑140 (MQ=255)  ttAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCgaga                                                                                                                                   >  1:201431/1‑140 (MQ=255)            aGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTaa                                                                                                                         <  1:723337/140‑1 (MQ=255)                         aTCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTg                                                                                                            >  2:746887/1‑140 (MQ=255)                              tttGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTAGATTGGGCtt                                                                                                       >  1:589930/1‑140 (MQ=255)                              tttGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGGGCATTCAGTTTCCTTAGTAAACCGGTAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCtt                                                                                                       >  1:814806/1‑140 (MQ=255)                                  ccATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAAt                                                                                                   <  2:43817/140‑1 (MQ=255)                                      gTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTAt                                                                                               >  1:36386/1‑140 (MQ=255)                                      gTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTAt                                                                                               >  2:609367/1‑140 (MQ=255)                                          ctactTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGttt                                                                                           >  2:775772/1‑140 (MQ=255)                                           tactTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGtttt                                                                                          >  1:232356/1‑140 (MQ=255)                                                tCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTc                                                                                     <  2:232356/140‑1 (MQ=255)                                                tCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACATAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTc                                                                                     >  1:573639/1‑140 (MQ=255)                                                 ccAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTcc                                                                                    >  1:259822/1‑140 (MQ=255)                                                     aCCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGAt                                                                                <  1:775772/140‑1 (MQ=255)                                                     aCCTGTAATTTTTACAGGTGCACTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGAt                                                                                <  1:609367/140‑1 (MQ=255)                                                       cTGTAATTTTTACAGGTGCATTCAGTTACCTTAGTAAACAGGAAGTAAATAAGATACGTAGTACATATAAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGa                                                                              <  2:814806/140‑1 (MQ=255)                                                         gTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGtttttt                                                                                        >  1:141384/1‑128 (MQ=255)                                                         gTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGtttttt                                                                                        <  2:141384/128‑1 (MQ=255)                                                          tAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAgtg                                                                           >  2:219381/1‑140 (MQ=255)                                                          tAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAgtg                                                                           >  2:95545/1‑140 (MQ=255)                                                                ttACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTc                                                                         <  1:676421/136‑1 (MQ=255)                                                                ttACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTc                                                                         >  2:676421/1‑136 (MQ=255)                                                                    aGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATAtgtg                                                                 >  1:516750/1‑140 (MQ=255)                                                                    aGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATAtgtg                                                                 >  2:15485/1‑140 (MQ=255)                                                                      gTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGaa                                                               <  1:219381/140‑1 (MQ=255)                                                                      gTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGaa                                                               <  1:95545/140‑1 (MQ=255)                                                                       tGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACATAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAAc                                                              <  2:573639/140‑1 (MQ=255)                                                                        gCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACg                                                             >  1:441561/1‑140 (MQ=255)                                                                           ttCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGaa                                                               <  1:502484/135‑1 (MQ=255)                                                                           ttCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGaa                                                               >  2:502484/1‑135 (MQ=255)                                                                           ttCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGaa                                                          <  1:15485/140‑1 (MQ=255)                                                                            tCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGaaa                                                         <  1:746887/140‑1 (MQ=255)                                                                              aGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAg                                                       <  2:201431/140‑1 (MQ=255)                                                                                 ttCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGaa                                                    >  1:452094/1‑140 (MQ=255)                                                                                     ttAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCa                                                <  2:380417/140‑1 (MQ=255)                                                                                        gTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATg                                             >  1:133175/1‑140 (MQ=255)                                                                                          aaaCCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGt                                           <  2:441561/140‑1 (MQ=255)                                                                                                           gATACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGaa                          >  1:423816/1‑140 (MQ=255)                                                                                                            aTACGTAGTACATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGaaa                         >  2:738591/1‑140 (MQ=255)                                                                                                                    taCATATCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGaaa                 >  2:582185/1‑140 (MQ=255)                                                                                                                        tatCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAatat             >  1:153381/1‑140 (MQ=255)                                                                                                                         atCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATAtt            >  1:793997/1‑140 (MQ=255)                                                                                                                          tCAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTg           <  2:805589/140‑1 (MQ=255)                                                                                                                           cAATAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGc          >  2:578202/1‑140 (MQ=255)                                                                                                                              tAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGCttt       >  2:233719/1‑140 (MQ=255)                                                                                                                              tAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGCttt       >  1:167467/1‑140 (MQ=255)                                                                                                                              tAAAGGTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGCttt       >  2:388447/1‑140 (MQ=255)                                                                                                                                   gTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGCTTTTTTGa  >  1:578359/1‑140 (MQ=255)                                                                                                                                   gTTAAGCGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGCTTTTTTGa  >  1:732769/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAACGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAAAAGGAATTCAATGGTTAAGTCAATATTTAGAAAAACTGAAAATATTGCTTTTTTGA  >  NC_000913/311295‑311564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑