Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,020,047	G→A	G296D (GGC→GAC)	ssnA →	putative aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,020,047	0	G	A	100.0%	132.3 / NA	38	G296D (GGC→GAC)	ssnA	putative aminohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/21);  total (17/21)

ATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGC  >  NC_000913/3019916‑3020166                                                                                                                                    |                                                                                                                        aTTNGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTNTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGNGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAAcc                                                                                                                 >  2:5194/1‑140 (MQ=255)  ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCa                                                                                                                <  1:128475/140‑1 (MQ=255)   tCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCAt                                                                                                               <  1:120551/140‑1 (MQ=255)   tCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCAt                                                                                                               >  2:33242/1‑140 (MQ=255)                   cAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATcc                                                                                               >  2:851225/1‑140 (MQ=255)                    aaaaCGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCg                                                                                              <  2:568154/140‑1 (MQ=255)                        cGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAAt                                                                                          <  1:252374/140‑1 (MQ=255)                           tGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTg                                                                                       <  2:650587/140‑1 (MQ=255)                              tCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCg                                                                                    <  1:851225/140‑1 (MQ=255)                                 cTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTg                                                                                 >  1:597786/1‑140 (MQ=255)                                  tCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTgg                                                                                >  2:591963/1‑140 (MQ=255)                                     tGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAATCATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGaa                                                                             <  1:231314/140‑1 (MQ=255)                                         cTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAacgga                                                                         <  2:23960/140‑1 (MQ=255)                                            tACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAacggacgg                                                                      >  1:800978/1‑140 (MQ=255)                                              cTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCa                                                                    <  2:597786/140‑1 (MQ=255)                                                    gAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGtt                                                              <  1:33242/140‑1 (MQ=255)                                                        gATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGa                                                          <  1:164662/140‑1 (MQ=255)                                                        gATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGa                                                          <  2:811755/140‑1 (MQ=255)                                                          tGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACa                                                        <  2:57799/140‑1 (MQ=255)                                                                        tCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATg                                          >  1:843127/1‑140 (MQ=255)                                                                         cAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGa                                         >  2:382957/1‑140 (MQ=255)                                                                           aTCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGaaa                                       >  1:364828/1‑140 (MQ=255)                                                                            tCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAgaga                                            <  2:368398/134‑1 (MQ=255)                                                                            tCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAgaga                                            <  2:558994/134‑1 (MQ=255)                                                                            tCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAgaga                                            >  1:558994/1‑134 (MQ=255)                                                                            tCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAgaga                                            >  1:368398/1‑134 (MQ=255)                                                                                cgcgATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGc                                  <  1:633826/140‑1 (MQ=255)                                                                                cgcgATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGc                                  <  1:591963/140‑1 (MQ=255)                                                                                cgcgATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGc                                  >  1:658601/1‑140 (MQ=255)                                                                                cgcgATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGc                                  <  1:382957/140‑1 (MQ=255)                                                                                    aTGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCcttc                              >  2:167147/1‑140 (MQ=255)                                                                                          tCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTCGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTaaa                        >  2:801610/1‑140 (MQ=255)                                                                                                  cATAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGa                <  1:5194/140‑1 (MQ=255)                                                                                                    tAACGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATg              >  1:449908/1‑140 (MQ=255)                                                                                                     aaCGCCCGTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATGc             <  1:167147/140‑1 (MQ=255)                                                                                                            gTTCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATGCGggtggt      >  1:423082/1‑140 (MQ=255)                                                                                                             ttCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATGCGGGTGGTc     >  1:53121/1‑140 (MQ=255)                                                                                                              tCAAACATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATGCGGGTGGTcc    >  1:668075/1‑140 (MQ=255)                                                                                                                aaaCATGAACAACCATGTCGACTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGc  <  2:658601/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                        ATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTCGGACATGTTTGAAGAGATGAAATTTGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGC  >  NC_000913/3019916‑3020166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑