Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,058,775	T→C	N184S (AAC→AGC)	rpiA ←	ribose‑5‑phosphate isomerase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,058,775	0	T	C	100.0%	153.3 / NA	43	N184S (AAC→AGC)	rpiA	ribose‑5‑phosphate isomerase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/19);  total (24/19)

TTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACT  >  NC_000913/3058638‑3058900                                                                                                                                          |                                                                                                                              ttttAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGctt                                                                                                                             >  1:563784/1‑140 (MQ=255)            gAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTAt                                                                                                                  <  1:262007/140‑1 (MQ=255)                     ccGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCggg                                                                                                              <  2:539388/135‑1 (MQ=255)                     ccGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCggg                                                                                                              >  1:539388/1‑135 (MQ=255)                     ccGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAg                                                                                                         <  2:779963/140‑1 (MQ=255)                      cGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAgg                                                                                                        >  2:10781/1‑140 (MQ=255)                        tCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGAt                                                                                                      >  2:208283/1‑140 (MQ=255)                          aGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGAttt                                                                                                    >  1:533273/1‑140 (MQ=255)                               aTTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATg                                                                                               >  1:338818/1‑140 (MQ=255)                                 ttCACAATGGTTTTGACGCCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGcc                                                                                             >  1:53394/1‑140 (MQ=255)                                   cacaATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGt                                                                                           >  1:8453/1‑140 (MQ=255)                                    acaATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTg                                                                                          <  2:8453/140‑1 (MQ=255)                                      aaTGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGa                                                                                        >  2:65549/1‑140 (MQ=255)                                              tGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAgg                                                                                <  2:338818/140‑1 (MQ=255)                                                acacCGTCAGGTATGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGAt                                                                              >  1:779418/1‑140 (MQ=255)                                                    cGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACg                                                                          <  2:533273/140‑1 (MQ=255)                                                    cGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACg                                                                          >  2:851139/1‑140 (MQ=255)                                                    cGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACg                                                                          <  2:563784/140‑1 (MQ=255)                                                    cGTCAGGTATGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACg                                                                          <  2:779418/140‑1 (MQ=255)                                                          gtgtGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCa                                                                    <  1:208283/140‑1 (MQ=255)                                                               ccAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATc                                                               >  2:419986/1‑140 (MQ=255)                                                                   tCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTc                                                           >  2:332522/1‑140 (MQ=255)                                                                       cgcAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTcacca                                                       <  2:53394/140‑1 (MQ=255)                                                                         cAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACg                                                     <  1:86904/140‑1 (MQ=255)                                                                            cGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGccc                                                  <  1:332522/140‑1 (MQ=255)                                                                            cGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGAGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGccc                                                  >  2:612145/1‑140 (MQ=255)                                                                               ccGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGa                                               <  1:84190/140‑1 (MQ=255)                                                                                       cGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGAGGGTATTc                                       >  1:635323/1‑140 (MQ=255)                                                                                            aGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGgac                                  >  2:143229/1‑140 (MQ=255)                                                                                                  cAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCg                                     <  1:8224/131‑1 (MQ=255)                                                                                                  cAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCg                                     >  2:8224/1‑131 (MQ=255)                                                                                                    aGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGccc                          >  1:865282/1‑140 (MQ=255)                                                                                                    aGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGccc                          >  1:433426/1‑140 (MQ=255)                                                                                                    aGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGccc                          >  2:704075/1‑140 (MQ=255)                                                                                                               accacGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAg               >  2:48893/1‑140 (MQ=255)                                                                                                               accacGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAg               >  1:47751/1‑140 (MQ=255)                                                                                                                ccacGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGc              >  2:545682/1‑140 (MQ=255)                                                                                                                  acGCCAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTg            <  1:65549/140‑1 (MQ=255)                                                                                                                     ccAGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGAcg         >  1:628845/1‑140 (MQ=255)                                                                                                                       aGGAATCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGAcgcg       <  1:419986/140‑1 (MQ=255)                                                                                                                       aGGAATCGCATTTATGGCGCTTTCCATCGCTATCGAGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGAcgcg       <  1:612145/140‑1 (MQ=255)                                                                                                                        ggAATGGCATTTATGGCCCTTCCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGCCGTCGAGGATCACGTGGCCATTATCGGTCCCCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCCCCAGCTGACgcgc      <  2:865282/140‑1 (MQ=255)                                                                                                                            tCGCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACt  <  1:851139/140‑1 (MQ=255)                                                                                                                              gCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGAcgcg       <  1:424882/133‑1 (MQ=255)                                                                                                                              gCATTTATGGCGCTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGAcgcg       >  2:424882/1‑133 (MQ=255)                                                                                                                                          |                                                                                                                              TTTTAACGGGGGAGGTTCCCCCGTCAGATCATTTCACAATGGTTTTGACACCGTCAGGTGTGCCAATCAGCGCAACGTCCGCGCCACGGTTAGCAAACAAGCCAACAGTCACCACGCCAGGAATCGCATTTATGGCGTTTTCCATCGCTATCGGGTCAAGGATTTCCATGCCGTGGACGTCGAGGATCACGTTGCCATTATCGGTCACCACGCCCTGACGGTATTCCGGACGACCGCCCAGTTTCACCAGCTGACGCGCCACT  >  NC_000913/3058638‑3058900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑