Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,063,580 T→C V198V (GTT→GTC)  argK → methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,063,5800TC100.0% 192.0 / NA 53V198V (GTT→GTC) argKmethylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/30);  total (23/30)

GAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTTATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCACTGGA  >  NC_000913/3063443‑3063711
                                                                                                                                         |                                                                                                                                   
gAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCAt                                                                                                                                   >  2:403230/1‑140 (MQ=255)
      gTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATcaacaa                                                                                                                             >  2:216577/1‑140 (MQ=255)
        tGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAg                                                                                                                           >  1:600912/1‑140 (MQ=255)
            gTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGa                                                                                                                       <  1:253103/140‑1 (MQ=255)
             tCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGAt                                                                                                                      <  2:47130/140‑1 (MQ=255)
                ggCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGc                                                                                                                   >  1:320605/1‑140 (MQ=255)
                  cAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGa                                                                                                                 <  1:182327/140‑1 (MQ=255)
                     tCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATaa                                                                                                              <  2:807567/140‑1 (MQ=255)
                     tCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATaa                                                                                                              <  2:446001/140‑1 (MQ=255)
                          aaCAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATa                                                                                                         <  2:124979/140‑1 (MQ=255)
                                   cGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCg                                                                                                >  2:394362/1‑140 (MQ=255)
                                    gCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGc                                                                                               >  2:803351/1‑140 (MQ=255)
                                      ccGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCa                                                                                             >  1:647957/1‑140 (MQ=255)
                                       cGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCAt                                                                                            <  1:701711/140‑1 (MQ=255)
                                         cATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTg                                                                                          <  1:394362/140‑1 (MQ=255)
                                           tggtggACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGcc                                                                                        <  2:647957/140‑1 (MQ=255)
                                           tggtggACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGcc                                                                                        <  2:149621/140‑1 (MQ=255)
                                              tggACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCgg                                                                                     >  2:107919/1‑140 (MQ=255)
                                               ggACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    <  2:320605/140‑1 (MQ=255)
                                               ggACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    <  1:311046/140‑1 (MQ=255)
                                               ggACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    <  2:145093/140‑1 (MQ=255)
                                               ggACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    <  1:107919/140‑1 (MQ=255)
                                               ggACTGTTTTATCTCGTTACAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    <  1:52707/140‑1 (MQ=255)
                                                      tttATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    >  2:446548/1‑133 (MQ=255)
                                                      tttATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    <  1:446548/133‑1 (MQ=255)
                                                      tttATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTa                                                                             <  2:482475/140‑1 (MQ=255)
                                                        tATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCat                                                                                  >  1:610612/1‑133 (MQ=255)
                                                        tATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCat                                                                                  <  2:610612/133‑1 (MQ=255)
                                                          tctcGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    <  1:248202/129‑1 (MQ=255)
                                                          tctcGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGc                                                                                    >  2:248202/1‑129 (MQ=255)
                                                            tcGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGagag                                                                       <  1:370312/140‑1 (MQ=255)
                                                               ttGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACga                                                                          <  2:545410/134‑1 (MQ=255)
                                                               ttGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACga                                                                          >  1:545410/1‑134 (MQ=255)
                                                                 gCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGccc                                                                  <  1:82423/140‑1 (MQ=255)
                                                                            gTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTg                                                       <  2:666960/140‑1 (MQ=255)
                                                                                       gatCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATa                                            <  1:403230/140‑1 (MQ=255)
                                                                                       gatCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATa                                            <  2:444267/140‑1 (MQ=255)
                                                                                             cAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATAcgacga                                      >  1:636968/1‑140 (MQ=255)
                                                                                                ggCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATg                                   >  2:150849/1‑140 (MQ=255)
                                                                                                  cATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGc                                 >  2:381637/1‑140 (MQ=255)
                                                                                                  cATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGc                                 >  1:847989/1‑140 (MQ=255)
                                                                                                   aTTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCa                                >  1:816271/1‑140 (MQ=255)
                                                                                                    ttAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAg                               >  2:421826/1‑140 (MQ=255)
                                                                                                     tAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATacgacg                                       >  1:151838/1‑131 (MQ=255)
                                                                                                     tAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATacgacg                                       <  2:151838/131‑1 (MQ=255)
                                                                                                     tAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGc                              >  2:200167/1‑140 (MQ=255)
                                                                                                          aaGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACgg                         >  2:18041/1‑140 (MQ=255)
                                                                                                           aGGGCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACggg                        <  1:18041/140‑1 (MQ=255)
                                                                                                            gggCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGt                       >  2:266076/1‑140 (MQ=255)
                                                                                                             ggCTGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGtt                      <  2:600912/140‑1 (MQ=255)
                                                                                                                tGATGGAAGTGGCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTg                   <  1:421826/140‑1 (MQ=255)
                                                                                                                           gCTGATCTGATCGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAgcgc        >  1:764032/1‑140 (MQ=255)
                                                                                                                                 ctgatcGTCATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCACTGGa  <  1:200167/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
GAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCATTAAAAAAGGGCTGATGGAAGTGGCTGATCTGATCGTTATCAACAAAGACGATGGCGATAACCATACCAATGTCGCCATTGCCCGGCATATGTACGAGAGTGCCCTGCATATTCTGCGACGTAAATACGACGAATGGCAGCCACGGGTTCTGACTTGTAGCGCACTGGA  >  NC_000913/3063443‑3063711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑