Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,105,587	A→G	intergenic (+75/‑127)	mltC → / → nupG	membrane‑bound lytic murein transglycosylase C/nucleoside:H(+) symporter NupG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,105,587	0	A	G	100.0%	180.6 / NA	50	intergenic (+75/‑127)	mltC/nupG	membrane‑bound lytic murein transglycosylase C/nucleoside:H(+) symporter NupG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/23);  total (27/23)

TCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGA  >  NC_000913/3105456‑3105717                                                                                                                                    |                                                                                                                                   tctcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTTCAAATTGGGGATGTTTGc                                                                                                                            >  1:489839/1‑140 (MQ=255) tccccccGTTATTTTTATAAAGTAAATACCGCGAAAAAACCCTCCCCCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGc                                                                                                                            <  1:127754/135‑1 (MQ=255)            tttttAAAAGGTAAATCCCGCGAAAAAATCCTCCCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGcc                                                                                                                 <  2:786517/138‑1 (MQ=255)             ttttATAAAGTGAATCCCGCGAAAAAATCCTCCCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCca                                                                                                                <  1:588429/139‑1 (MQ=255)             ttttACAAATTCAATCCCGCACAAAAATCCTCCCGCCCCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCCCAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCca                                                                                                                <  2:741587/139‑1 (MQ=255)                     aGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAAc                                                                                                        >  1:262583/1‑140 (MQ=255)                       tGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAA‑TTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACaaa                                                                                                     >  1:622745/1‑140 (MQ=255)                          aTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACaaaaa                                                                                                   >  2:651730/1‑140 (MQ=255)                          aTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACaaaaa                                                                                                   >  1:607464/1‑140 (MQ=255)                                cgcAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGt                                                                                             >  2:554881/1‑140 (MQ=255)                                cgcAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGt                                                                                             >  2:264840/1‑140 (MQ=255)                                     aaaCCCTCCCCCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCcgcg                                                                                        <  2:411150/140‑1 (MQ=255)                                        tCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTTCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAg                                                                                     <  2:489839/140‑1 (MQ=255)                                                 cgccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGaa                                                                            <  1:651730/140‑1 (MQ=255)                                                 ccccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGaa                                                                            <  1:236137/138‑1 (MQ=255)                                                  gccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAAt                                                                           >  1:627634/1‑140 (MQ=255)                                                     gATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATccc                                                                        <  2:262583/140‑1 (MQ=255)                                                     gATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATccc                                                                        <  2:627634/140‑1 (MQ=255)                                                      aTAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCca                                                                       <  1:364256/140‑1 (MQ=255)                                                      aTAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCca                                                                       <  2:113309/140‑1 (MQ=255)                                                               ttAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAA‑TTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCa                                                             <  2:622745/140‑1 (MQ=255)                                                                tAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCa                                                             >  1:618384/1‑140 (MQ=255)                                                                     gCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTc                                                        >  1:816558/1‑140 (MQ=255)                                                                       cccTGACGATGCTCAGGAGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCaa                                                      >  1:115546/1‑140 (MQ=255)                                                                            aCGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgtg                                                 <  2:816558/140‑1 (MQ=255)                                                                            aCGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgtg                                                 >  1:467790/1‑140 (MQ=255)                                                                              gATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCt                                               <  1:264840/140‑1 (MQ=255)                                                                              gATGCTCAGGGGCAAAAATGTTATCCACATCACAAGTACGTTTTGCAAAGTGGGGATGTTTGCAAATATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCt                                               <  2:115546/140‑1 (MQ=255)                                                                                 gCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCtttt                                            >  1:268838/1‑140 (MQ=255)                                                                                   tCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTc                                          >  1:304557/1‑140 (MQ=255)                                                                                     aGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCaa                                        >  1:852051/1‑140 (MQ=255)                                                                                      ggggCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGt                                                         >  2:263498/1‑122 (MQ=255)                                                                                      ggggCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGt                                                         <  1:263498/122‑1 (MQ=255)                                                                                      ggggCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCaaa                                       >  1:153569/1‑140 (MQ=255)                                                                                             aaaTGTTATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCAt                                <  2:607464/140‑1 (MQ=255)                                                                                                  ttATCCACATCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCa                           >  1:602024/1‑140 (MQ=255)                                                                                                        acaTCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGa                     >  1:159034/1‑140 (MQ=255)                                                                                                          aTCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGAtg                   >  2:376308/1‑140 (MQ=255)                                                                                                          aTCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGAtg                   >  1:372781/1‑140 (MQ=255)                                                                                                          aTCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGAtg                   >  2:511076/1‑140 (MQ=255)                                                                                                          aTCACAATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGAtg                   >  2:817660/1‑140 (MQ=255)                                                                                                            cacaATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGAtgtg                 >  2:480568/1‑140 (MQ=255)                                                                                                            cacaATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGAtgtg                 >  2:496005/1‑140 (MQ=255)                                                                                                              caATTTCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAg               <  2:467790/140‑1 (MQ=255)                                                                                                                  ttCGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGaaa           <  2:372781/140‑1 (MQ=255)                                                                                                                    cGTTTTGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAAtt         <  1:511076/140‑1 (MQ=255)                                                                                                                      ttttGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTaa       >  1:587418/1‑140 (MQ=255)                                                                                                                         tGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAATTGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACAt    <  1:718506/140‑1 (MQ=255)                                                                                                                         tGCAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACAt    <  2:602024/140‑1 (MQ=255)                                                                                                                           cAAATTGGGGATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGa  >  1:530526/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                   TCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAACACTCATCCGCATCACGATGTGAGGAAATTAACATGA  >  NC_000913/3105456‑3105717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑