Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,941	G→A	G97G (GGC→GGT)	glcG ←	putative heme‑binding protein GlcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,941	0	G	A	100.0%	105.9 / NA	32	G97G (GGC→GGT)	glcG	putative heme‑binding protein GlcG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/14);  total (18/14)

TTGTTTATCTCCTCGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCG  >  NC_000913/3123805‑3124071                                                                                                                                         |                                                                                                                                   ttGTTTATCTCCTCGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAGCAACCGGTACGCCACCt                                                                                                                                 >  2:32020/1‑140 (MQ=255)        tctcCTCGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCg                                                                                                                          <  1:810076/140‑1 (MQ=255)            ctcGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGt                                                                                                                      >  2:226214/1‑140 (MQ=255)            ctcGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGt                                                                                                                      >  1:255371/1‑140 (MQ=255)            ctcGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGt                                                                                                                      >  1:257007/1‑140 (MQ=255)            ctcGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGt                                                                                                                      >  1:501157/1‑140 (MQ=255)                       ttATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAGCAACCGGTACGCCACCTTCCAGCGACGTTAATAACGgcg                                                                                                           <  1:32020/140‑1 (MQ=255)                       ttATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGgcg                                                                                                           <  2:257007/140‑1 (MQ=255)                             cGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCa                                                                                                     <  2:468167/140‑1 (MQ=255)                               ccAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACg                                                                                                   >  2:607385/1‑140 (MQ=255)                                  acacCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAAc                                                                                                <  2:255371/140‑1 (MQ=255)                                    acCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAAcgc                                                                                              <  1:226214/140‑1 (MQ=255)                                              ccGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCg                                                                                    <  1:544962/140‑1 (MQ=255)                                                    tCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTc                                                                              <  1:790634/140‑1 (MQ=255)                                                          ccTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCAtc                                                                        <  2:501157/140‑1 (MQ=255)                                                                cATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCa                                                                  <  1:770658/140‑1 (MQ=255)                                                                cATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCa                                                                  <  2:19431/140‑1 (MQ=255)                                                                                 aaaCCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCacg                                                 >  2:706119/1‑140 (MQ=255)                                                                                            gCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAgcg                                      <  1:121271/140‑1 (MQ=255)                                                                                               cACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCgcgg                                   >  1:152177/1‑140 (MQ=255)                                                                                                  ggCACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGcggcgg                                >  1:714335/1‑140 (MQ=255)                                                                                                    cACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTa                              >  1:570982/1‑140 (MQ=255)                                                                                                    cACCAATAATTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTa                              >  1:272346/1‑140 (MQ=255)                                                                                                          taatTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCt                        <  2:152177/140‑1 (MQ=255)                                                                                                            atTTGCCCATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCttt                      >  2:510818/1‑140 (MQ=255)                                                                                                                 cccATCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCt                 >  1:375333/1‑140 (MQ=255)                                                                                                                     tCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTggg              <  1:630567/139‑1 (MQ=255)                                                                                                                     tCCACAACAACCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTggg              >  2:630567/1‑139 (MQ=255)                                                                                                                           acaacCGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGAtata       >  2:467170/1‑140 (MQ=255)                                                                                                                               ccGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGcc   >  2:204905/1‑140 (MQ=255)                                                                                                                               ccGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGcc   >  2:526422/1‑140 (MQ=255)                                                                                                                                cGGTACGCCACCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCg  >  1:338666/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TTGTTTATCTCCTCGTTTTCGCTTATTTCGCCAACACCGCTGCTGCCGCTTTCGCGACCTGCGCATCCTGTGCTCCGGTTAAACCAGAAACGCCCACGGCACCAATAATTTGCCCATCCACAACAACCGGTACGCCGCCTTCCAGCGACGTTAATAACGGCGCAGTCACGAACGCGGTACGTCCGTTGTTCACCATCTCTTCATAGCCCTTAGTTTCACGACGCCCCAGCGCGGCGGTACGCGCTTTCTCCTGGGAGATATAAGCCG  >  NC_000913/3123805‑3124071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑